76 research outputs found
Studies on the effect of unequal flowering on the effective population number in Danish seed orchard crops
Nuclear Vav3 is required for polycomb repression complex-1 activity in B-cell lymphoblastic leukemogenesis
Acute B-cell lymphoblastic leukemia (B-ALL) results from oligo-clonal evolution of B-cell progenitors endowed with initiating and propagating leukemia properties. The activation of both the Rac guanine nucleotide exchange factor (Rac GEF) Vav3 and Rac GTPases is required for leukemogenesis mediated by the oncogenic fusion protein BCR-ABL. Vav3 expression becomes predominantly nuclear upon expression of BCR-ABL signature. In the nucleus, Vav3 interacts with BCR-ABL, Rac, and the polycomb repression complex (PRC) proteins Bmi1, Ring1b and Ezh2. The GEF activity of Vav3 is required for the proliferation, Bmi1-dependent B-cell progenitor self-renewal, nuclear Rac activation, protein interaction with Bmi1, mono-ubiquitination of H2A(K119) (H2AK119Ub) and repression of PRC-1 (PRC1) downstream target loci, of leukemic B-cell progenitors. Vav3 deficiency results in de-repression of negative regulators of cell proliferation and repression of oncogenic transcriptional factors. Mechanistically, we show that Vav3 prevents the Phlpp2-sensitive and Akt (S473)-dependent phosphorylation of Bmi1 on the regulatory residue S314 that, in turn, promotes the transcriptional factor reprogramming of leukemic B-cell progenitors. These results highlight the importance of non-canonical nuclear Rho GTPase signaling in leukemogenesis.This project was funded by the National Institutes of Health Grants R01-CA273016 (N.N.N. and J.A.C.) and U54-DK126108 (J.A.C.), the Leukemia & Lymphoma Society of North America (J.A.C and N.N.N.; and N.N.N. and J.A.C.), and William Lawrence & Blanche Hughes Foundation (J.A.C. and N.N.)
A ¤partial linkage map of Picea abies clone V6470 based on recombination of RAPD-markers in haploid megagametophytes
Selektion af sunde rødgran:rapport til Statens Planteavlsstation, Statens Jordbrugs- og Veterinærvidenskabelige Forskningsråd og Det Strategiske Miljøforskningsprogram
Design of QTL-experiments with the aim of implementation of Marker Aided Selection - MAS - in breeding of Picea abies
Application of RAPD for high-density genome mapping and markeraided selection in Norway spruce
Variation in flowering and reproductive success in a Danish Picea abies (KARST.) seed orchard
Application of RAPD for high density genome mapping and search for linkage to QTL in Norway sprice (Picea abies)
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