58 research outputs found
Can a genetic signature for metastatic head and neck squamous cell carcinoma be characterised by comparative genomic hybridisation?
Detection of occult carcinomatous diffusion in lymph nodes from head and neck squamous cell carcinoma using real-time RTâPCR detection of cytokeratin 19 mRNA
The aim of the present study was to evaluate the occult lymph node carcinomatous diffusion in head and neck squamous cell carcinoma (HNSCC). A total of 1328 lymph nodes from 31 patients treated between 2004 and 2005 were prospectively evaluated by routine haematoxylinâeosinâsafran (HES) staining, immunohistochemistry (IHC) and real-time Taqman reverseâtranscriptase polymerase chain reaction (real-time RTâPCR) assay. Amplification of cytokeratin 19 (CK19) mRNA transcripts using real-time RTâPCR was used to quantify cervical micrometastatic burden. The cervical lymph node metastatic rates determined by routine HES staining and real-time RTâPCR assay were 16.3 and 36.0%, respectively (P<0.0001). A potential change in the nodal status was observed in 13 (42.0%) of the 31 patients and an atypical pattern of lymphatic spread was identified in four patients (12.9%). Moreover, CK19 mRNA expression values in histologically positive lymph nodes were significantly higher than those observed in histologically negative lymph nodes (P<0.0001). These results indicate that real-time RTâPCR assay for the detection of CK19 mRNA is a sensitive and reliable method for the detection of carcinomatous cells in lymph nodes. This type of method could be used to reassess lymph node status according to occult lymphatic spread in patients with HNSCC
The expression of SOCS1 and TLR4-NFkappaB pathway molecules in neoplastic cells as potential biomarker for the aggressive tumor phenotype in laryngeal carcinoma.
Untersuchungen zur AggressivitÀtsabschÀtzung und zur Angiogenese bei adenomatösen Neoplasien des Mittelohres
Treatment for postchemoradiotherapy hypopharyngeal stenosis: Pharyngoesophageal bypass using an anterolateral thigh flapâA case report
Ergebnisse der Nasalen Provokation in der Diagnostik der allergischen Rhinitis bei Kindern
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