Time Course of PR of UV-Induced Chromosomal Aberrations and Lethal Damage in S and G2 Xenopus Cells

Sand G2 phase cells were exposed to 150 ergs mm⁻² UV and their ability to photoreactivate the induced cell killing (loss of colony forming ability) and chromosomal aberrations was determined as a function of time following the UV exposure. In S phase cells, the lesions leading to cell death and those leading to aberrations were both converted to a non-photoreactivable state shortly after the UV exposure. A significant fraction of the lesions induced in G2 cells, that led to cell death, were converted to a non-photoreactivable state before the progeny of the exposed cells reached the next succeeding S phase. Few, if any, lesions were induced in G2 cells that were expressed as aberrations at the first mitosis following exposure. Some of the lesions induced in G2 cells led to aberrations that were observable in the progeny that progressed to the second mitosis following exposure. These lesions were converted to a nonphotoreactivable state as the progeny of the exposed G2 cells progressed through the first S phase following exposure

Time Course of PR of UV-Induced Chromosomal Aberrations and Lethal Damage in G1 Xenopus Cells

Synchronous cultures of early G1 cells were exposed to UV and their ability to photoreactivate lethal and aberrational damage was determined as a function of time following UV exposure. Lesions leading to cell death were converted to a non-photoreactivable state before cells entered S phase, while lesions leading to chromosomal aberrations were converted to a non-photoreactivable state as the cells entered S phase. These results indicate that the intracellular mechanism which expresses photoreactivable UV-induced lesions in G1 cells as cell death is not identical to the mechanism which expresses such lesions as chromosomal aberrations, and the two mechanisms operate primarily in different phases of the cell cycle

HI Observations of the starburst galaxy NGC 2146

NGC 2146 is a peculiar spiral galaxy which is currently undergoing a major burst of star formation and is immersed in a extended HI structure that has morphological and kinematical resemblence to a strong tidal interaction. This paper reports aperture synthesis observations carried out in the 21cm line with the Very Large Array (VLA - The National Radio Astronomy Observatory (NRAO) is operated by Associated Universities, Inc. under cooperative agreement with the National Science Foundation.) of two fields positioned to optimally cover the HI streams to the north and south of the galaxy, along with a 300 ft total power spectral mapping program to recover the low surface brightness extended emission. The observations reveal elongated streams of neutral hydrogen towards both the north and the south of the optical galaxy extending out up to 6 Holmberg radii. The streams are not in the principle plane of rotation of the galaxy, but instead are suggestive of a tidal interaction between NGC 2146 and a LSB companion that was destroyed by the encounter and remains undetected at optical wavelengths. Part of the southern stream is turning back to fall into the main galaxy, where it will create a long-lived warp in the HI disk of NGC 2146. Analysis of the trajectory of the outlying gas suggests that the closest encounter took place about 0.8 billion years ago and that infall of debris will continue for a similar time span.Comment: To be published in A&

Development of lithium diffused radiation resistant solar cells, part 1 Final report, 17 Jun. 1969 - 19 Jun. 1970

Development of lithium diffused radiation resistant solar cell

Empirical Traffic Data and Their Implications for Traffic Modeling

From single vehicle data a number of new empirical results about the temporal evolution, correlation, and density-dependence of macroscopic traffic quantities have been determined. These have relevant implications for traffic modeling and allow to test existing traffic models.Comment: For related work see http://www.theo2.physik.uni-stuttgart.de/helbing.htm

Reproducibility of cognitive endpoints in clinical trials: Lessons from neurofibromatosis type 1

OBJECTIVE: Rapid developments in understanding the molecular mechanisms underlying cognitive deficits in neurodevelopmental disorders have increased expectations for targeted, mechanism-based treatments. However, translation from preclinical models to human clinical trials has proven challenging. Poor reproducibility of cognitive endpoints may provide one explanation for this finding. We examined the suitability of cognitive outcomes for clinical trials in children with neurofibromatosis type 1 (NF1) by examining test-retest reliability of the measures and the application of data reduction techniques to improve reproducibility. METHODS: Data were analyzed from the STARS clinical trial (n = 146), a multi-center double-blind placebo-controlled phase II trial of lovastatin, conducted by the NF Clinical Trials Consortium. Intra-class correlation coefficients were generated between pre- and post-performances (16-week interval) on neuropsychological endpoints in the placebo group to determine test-retest reliabilities. Confirmatory factor analysis was used to reduce data into cognitive domains and account for measurement error. RESULTS: Test-retest reliabilities were highly variable, with most endpoints demonstrating unacceptably low reproducibility. Data reduction confirmed four distinct neuropsychological domains: executive functioning/attention, visuospatial ability, memory, and behavior. Test-retest reliabilities of latent factors improved to acceptable levels for clinical trials. Applicability and utility of our model was demonstrated by homogeneous effect sizes in the reanalyzed efficacy data. INTERPRETATION: These data demonstrate that single observed endpoints are not appropriate to determine efficacy, partly accounting for the poor test-retest reliability of cognitive outcomes in clinical trials in neurodevelopmental disorders. Recommendations to improve reproducibility are outlined to guide future trial design

Breast feeding practices and views among diabetic women: a retrospective cohort study

Objective: to explore the pattern and experiences of breast-feeding practices among diabetic women. Design: retrospective cohort study using maternal records and postal questionnaires in a Baby-Friendly hospital. Participants: diabetic mothers including women with gestational diabetes, and type 1 and 2 diabetes mellitus. Findings: from the total group of respondents, 81.9% intended to breast feed. The actual breast feeding rates were 81.9% at birth, 68.1% at 2 weeks and 28.7% at 6 months postpartum. Major themes that were identified from women's experiences included information and advice, support vs. pressure, classification and labelling, and expectations. Conclusions: more than two-thirds of the diabetic women intended to breast feed and actually did breast feed in this study. For both the total study population and the type 1 and 2 diabetics alone, more than half were still breast feeding at 2 weeks postpartum, and approximately one-third were still breast feeding at 6 months postpartum. Implications for practice: structured support, provided for women through Baby-Friendly initiatives, was appreciated by the diabetic women in this study. The extent to which this support influenced the highly successful breast feeding practices in this group of women needs focused investigation. The need for a delicate balancing act between pressure and advice in order to prevent coercion was noted.</p

Fuel optimum stochastic attitude control

Numerical solution of stochastic Hamilton-Jacobi equation for fuel optimal spacecraft attitude control syste