14 research outputs found
Ratio of invaded to removed lymph nodes as a prognostic factor in adenocarcinoma of the distal esophagus and esophagogastric junction
A state observation and torque compensation–based acceleration slip regulation control approach for a four-wheel independent drive electric vehicle under slope driving
Catalytic properties of immobilized tannase produced from Aspergillus aculeatus compared with the free enzyme
Aspergillus aculeatus tannase was immobilized on several carriers by entrapment and covalent binding with cross - linking. Tannase immobilized on gelatin with cross - linking agent showed the highest activity and immobilization yield. The optimum pH of the immobilized enzyme was shifted to a more acidic range compared with the free enzyme (from pH 5.5 to pH 5.0). The optimum temperature of the reaction was determined to be 50ºC for the free enzyme and 60ºC for the immobilized form. The thermal stability, as well as stability over a wide range of pH, was significantly improved by the immobilization process. The calculated Km of the immobilized tannase (11.8 mg ml-1) is higher than that of the free tannase (6.5 mg ml-1), while Vmax of the immobilized enzyme (0.32 U (µg protein)-1) is lower than that of the free tannase (2.7 U (µg protein)-1). The immobilized enzyme was able to retain 84 % of the initial catalytic activity after 5.0 cycles