Eastern Pine Seedworm, \u3ci\u3eCydia Toreuta\u3c/i\u3e (Lepidoptera: Tortricidae) in Red Pine Cones in Wisconsin

Cydia toreuta population densities, prolonged diapause behavior, parasitism and adult emergence patterns were examined over four years at two red pine locations in Wisconsin. Last-instar densities ranged from 0.54 to 3.18 per cone. This was considered a wide range for this species in red pine. Population clumping was evident at last-instar densities below 2.90, however no consistent pattern was evident between years. Clumping disappeared at populations greater than 2.90 last-instars. Prolonged diapause varied from 7.8 to 38.9070. Parasitism rates var­ ied from 10.9 to 46.6 %. Phanerotoma toreuta (Hymenoptera: Braconidae) was the most abundant parasite at both sites and emerged in unison with male C. toreuta. Estimation of percent of last-instars undergoing prolonged diapause prior to spring emergence can be accomplished using forced emergence though cones should be collected after 31 January. Estimation of percent parasitism can be made as early as November. Resident moth populations could be estimated prior to spring flight utilizing this information. Emergence occurred over an approximately 2 week period between mid-May and early June. The majority of male moths emerged prior to females

Carrion Beetles (Coleoptera: Silphidae) of Wisconsin

The first comprehensive faunal survey of the carrion beetles (Coleoptera: Silphidae) of Wisconsin is presented. Six genera and 14 species are recorded from the state, including a new state record, Heterosilpha ramosa (Say). Nicrophorus americanus Olivier was not recovered during this study. An annotated checklist includes species-specific geographical and temporal distributions, remarks on foods and habitat, and counties of specimen collections for each species

Carrion Beetles (Coleoptera: Silphidae) of Wisconsin

The first comprehensive faunal survey of the carrion beetles (Coleoptera: Silphidae) of Wisconsin is presented. Six genera and 14 species are recorded from the state, including a new state record, Heterosilpha ramosa (Say). Nicrophorus americanus Olivier was not recovered during this study. An annotated checklist includes species-specific geographical and temporal distributions, remarks on foods and habitat, and counties of specimen collections for each species

Complementary Embryonic and Adult Cell Populations Enhance Myocardial Repair in Rat Myocardial Injury Model

We compared the functional outcome of Isl-1+ cardiac progenitors, CD90+ bone marrow-derived progenitor cells, and the combination of the two in a rat myocardial infarction (MI) model. Isl-1+ cells were isolated from embryonic day 12.5 (E12.5) rat hearts and expanded in vitro. Thy-1+/CD90+ cells were isolated from the bone marrow of adult Sprague-Dawley rats by immunomagnetic cell sorting. Six-week-old female Sprague-Dawley rats underwent permanent left anterior descending (LAD) coronary artery ligation and received intramyocardial injection of either saline, Isl-1+ cells, CD90+ cells, or a combination of Isl-1+ and CD90+ cells, at the time of infarction. Cells were delivered transepicardially to the peri-infarct zone. Left ventricular function was assessed by transthoracic echocardiography at 1- and 4-week post-MI and by Millar catheterization (-dP/dt and +dP/dt) at 4-week post-MI. Fluorescence in situ hybridization (Isl-1+cells) and monochrystalline iron oxide nanoparticles labeling (MION; CD90+ cells) were performed to assess biodistribution of transplanted cells. Only the combination of cells demonstrated a significant improvement of cardiac function as assessed by anterior wall contractility, dP/dt (max), and dP/dt (min), compared to Isl-1+ or CD90+ cell monotherapies. In the combination cell group, viable cells were detected at week 4 when anterior wall motion was completely restored. In conclusion, the combination of Isl-1+ cardiac progenitors and adult bone marrow-derived CD90+ cells shows prolonged and robust myocardial tissue repair and provides support for the use of complementary cell populations to enhance myocardial repair