HTS SQUID System for Eddy Current Testing of Airplane Wheels and Rivets

Nondestructive testing (NDT) of new and aging aircraft structures is essential for flight safety. Inspection costs can be reduced by using an inspection technique with high sensitivity for small flaws. Of the many NDT methods being used in aircraft maintenance, eddy-current testing is well established, especially for layered structures. Nevertheless, some test tasks cannot be assured with conventional eddy current systems with sufficient sensitivity and dynamic range. Superconducting Quantum Interference Devices (SQUIDs) are the most sensitive magnetic field sensors known to date. With the discovery of High Temperature Superconductors (HTS) ten years ago and the subsequent development of HTS SQUIDs requiring only cooling down to liquid nitrogen temperature, the greatest application barrier appears solvable. SQUID systems offer a high sensitivity at low excitation frequencies, permitting the detection of deeper flaws, and a high linearity, allowing quantitative evaluation of magnetic field maps from the investigated structure [1–3]. The potential of eddy current testing with HTS SQUIDs has previously been demonstrated for up to 5 cm deep-lying defects in stacks of aluminum sheets using a stationary axial SQUID gradiometer [4]. Kreutzbruck et al. [5] performed a direct comparison between a SQUID magnetometer system and a conventional eddy current testing unit (Elotest Bl of Rohmann GmbH), with a well defined saw cut in a plate of aircraft aluminum alloy hidden under a stack of flawless aluminum plates. They demonstrated an improvement in signal-to-noise ratio of approximately 150, when comparing the SQUID signature of the slot with the conventional system

The dose makes the poison: have “field realistic” rates of exposure of bees to neonicotinoid insecticides been overestimated in laboratory studies?

Recent laboratory based studies have demonstrated adverse sub-lethal effects of neonicotinoid insecticides on honey bees and bumble bees, and these studies have been influential in leading to a European Union moratorium on the use of three neonicotinoids, clothianidin, imidacloprid, and thiamethoxam on “bee attractive” crops. Yet so far, these same effects have not been observed in field studies. Here we review the three key dosage factors (concentration, duration and choice) relevant to field conditions, and conclude that these have probably been over estimated in many laboratory based studies

Nicotine signals through muscle-type and neuronal nicotinic acetylcholine receptors in both human bronchial epithelial cells and airway fibroblasts

BACKGROUND: Non-neuronal cells, including those derived from lung, are reported to express nicotinic acetylcholine receptors (nAChR). We examined nAChR subunit expression in short-term cultures of human airway cells derived from a series of never smokers, ex-smokers, and active smokers. METHODS AND RESULTS: At the mRNA level, human bronchial epithelial (HBE) cells and airway fibroblasts expressed a range of nAChR subunits. In multiple cultures of both cell types, mRNA was detected for subunits that constitute functional muscle-type and neuronal-type pentomeric receptors. Two immortalized cell lines derived from HBE cells also expressed muscle-type and neuronal-type nAChR subunits. Airway fibroblasts expressed mRNA for three muscle-type subunits (α1, δ, and ε) significantly more often than HBE cells. Immunoblotting of HBE cell and airway fibroblast extracts confirmed that mRNA for many nAChR subunits is translated into detectable levels of protein, and evidence of glycosylation of nAChRs was observed. Some minor differences in nAChR expression were found based on smoking status in fibroblasts or HBE cells. Nicotine triggered calcium influx in the immortalized HBE cell line BEAS2B, which was blocked by α-bungarotoxin and to a lesser extent by hexamethonium. Activation of PKC and MAPK p38, but not MAPK p42/44, was observed in BEAS2B cells exposed to nicotine. In contrast, nicotine could activate p42/44 in airway fibroblasts within five minutes of exposure. CONCLUSIONS: These results suggest that muscle-type and neuronal-type nAChRs are functional in airway fibroblasts and HBE cells, that prior tobacco exposure does not appear to be an important variable in nAChR expression, and that distinct signaling pathways are observed in response to nicotine

CAR-T cell. the long and winding road to solid tumors

Adoptive cell therapy of solid tumors with reprogrammed T cells can be considered the "next generation" of cancer hallmarks. CAR-T cells fail to be as effective as in liquid tumors for the inability to reach and survive in the microenvironment surrounding the neoplastic foci. The intricate net of cross-interactions occurring between tumor components, stromal and immune cells leads to an ineffective anergic status favoring the evasion from the host's defenses. Our goal is hereby to trace the road imposed by solid tumors to CAR-T cells, highlighting pitfalls and strategies to be developed and refined to possibly overcome these hurdles