Plant materials used for the study of morphological and molecular (SSR) variation of a collection of eggplants from China, Spain, and Sri Lanka.

<p>Plant materials used for the study of morphological and molecular (SSR) variation of a collection of eggplants from China, Spain, and Sri Lanka.</p

PCA SSR-based relationships between accessions.

<p>A total of 52 <i>S. melongena</i> accessions from China (white squares), Spain (grey triangles), and Sri Lanka (black circles) (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0041748#pone-0041748-t001" target="_blank">Table 1</a>) were evaluated using 12 polymorphic SSRs (see text and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0041748#pone-0041748-t003" target="_blank">Tables 3</a> and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0041748#pone-0041748-t005" target="_blank">5</a>) and were represented on the three first components (PC1, PC2 and PC3) of the principal components analysis (22.0%, 19.1%, and 17.9% of the total variation explained by the first, second, and third principal components, respectively). Scatterplots show the projections of the accessions on the first and second principal components (above) and on the first and third principal components (below).</p

PCA relationships between morphological traits.

<p>The two first components (PC1 and PC2) of the principal components analysis account for 22.9% and 16.2% of the total variation, respectively. Results were obtained after the characterization of 52 <i>S. melongena</i> accessions from China, Spain, and Sri Lanka using 28 morphological descriptors (see text and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0041748#pone-0041748-t002" target="_blank">Table 2</a>).</p

Morphological traits measured in a scale with pre-determined values of the descriptor states, and description of the scale used for the study of morphological variation in the eggplant accessions studied [4], [19], [30].

<p>Morphological traits measured in a scale with pre-determined values of the descriptor states, and description of the scale used for the study of morphological variation in the eggplant accessions studied <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0041748#pone.0041748-MuozFalcn1" target="_blank">[4]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0041748#pone.0041748-Prohens1" target="_blank">[19]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0041748#pone.0041748-vanderWeerden1" target="_blank">[30]</a>.</p

Mean and range for the traits measured in the eggplant accessions originating from China, Spain, and Sri Lanka used for the present study, and probability of the <i>F</i>-statistic, obtained from ANOVA analyses, for differences among means.

a<p>Means separated by different letters within a row are significantly different according to the Student-Newman-Keuls multiple range test at P≤0.05.</p

PCA morphology-based relationships between accessions.

<p>A total of 52 <i>S. melongena</i> accessions from China (white squares), Spain (grey triangles), and Sri Lanka (black circles) (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0041748#pone-0041748-t001" target="_blank">Table 1</a>) were morphologically evaluated with 28 morphological traits (see text and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0041748#pone-0041748-t002" target="_blank">Table 2</a>) and were represented on the two first components (PC1 and PC2) of the principal components analysis (22.9% and 16.2% of the total variation explained by the first and second component, respectively).</p

SSR alleles in the different origins.

<p>The figures indicate the number of SSR alleles private to each of the secondary centers of diversity, as well as the number of alleles shared by accessions from two or the three different origins.</p

Primer sequences, expected size, annealing temperature, linkage group, and map position [35], [39] of the twelve SSR markers used for molecular characterization of the materials studied.

a<p>Markers CSM27 and CSM44 are positioned at 10.8 cM and 88.7 cM, respectively, in linkage group 3.</p>b<p>Markers CSM30, CSM36 and CSM54 are positioned at 29.3 cM, 71.2 cM, and 0.0 cM, respectively, in linkage group 9.</p>c<p>Markers CSM31 and CSM43 are positioned at 182.0 cM and 161.8 cM, respectively, in linkage group 1.</p

SSR markers, number of alleles per locus for all the samples and for each origin (China, Spain, and Sri Lanka), number of private alleles (i.e., present in one or more accessions of each origin), and PIC value for each SSR locus.

<p>SSR markers, number of alleles per locus for all the samples and for each origin (China, Spain, and Sri Lanka), number of private alleles (i.e., present in one or more accessions of each origin), and PIC value for each SSR locus.</p

SSR-based estimates of genetic distances (above the diagonal) and genetic identities (below the diagonal) between different eggplant origins.

<p>SSR-based estimates of genetic distances (above the diagonal) and genetic identities (below the diagonal) between different eggplant origins.</p