Immune response outcome of total 118 exposures for all 49 macaques receiving F(ab΄)₂ radiotracers.

<p>Immune response outcome of total 118 exposures for all 49 macaques receiving F(ab΄)₂ radiotracers.</p

Correlation between radio-HPLC and plasma binding assay.

<p>Strong correlation was observed between plasma binding assay and HPLC assay. Samples were assayed with either F(ab΄)₂-CD4R1 antibody (brown dots) or F(ab΄)₂-huOKT4A antibody (red dots). For samples assayed with F(ab΄)₂-CD4R1, the bootstrap estimate and 95% confidence interval of the Spearman rank correlation between PBA-ratio and HPLC-HM-ratio was -0.88 (-0.91, -0.84), and between PBA-ratio and HPLC-Fab’2-ratio was 0.90 (0.85, 0.93). Similar results were observed for samples assayed with F(ab΄)₂-huOKT4A.</p

Maximum intensity projection SPECT images.

<p>Rhesus macaques that developed antibody response to the radiotracer after baseline exposure were subsequently imaged while anti-tracer antibodies were present in the plasma in (A) healthy macaque imaged at 48 hours post-radiotracer injection with intact-huOKT4A labelled with ¹¹¹In and scanned using Triad88 (Trionix) camera, (B) SIV-TK infected non-progressor imaged at 4 hours post-radiotracer injection with F(ab΄)₂-huOKT4A labelled with ^99mTc and scanned using Triad88 (Trionix) camera, and (C) healthy macaque that underwent total body irradiation prior to 2^nd exposure and imaged at 4 hours post-radiotracer injection with F(ab΄)₂-CD4R1 labelled with ^99mTc and scanned using Symbia T2 (Siemens) camera. Images show increased hepatic uptake and altered biodistribution with minimal to no binding observed in secondary lymphoid organs when imaged in the presence of anti-tracer antibodies. Within each panel, images were adjusted (standardized) for injected dose and body weight. Tissue uptakes were converted to RAINBOW color scale as shown in color bar.</p