5 research outputs found
2s Hyperfine Structure in Hydrogen Atom and Helium-3 Ion
The usefulness of study of hyperfine splitting in the hydrogen atom is
limited on a level of 10 ppm by our knowledge of the proton structure. One way
to go beyond 10 ppm is to study a specific difference of the hyperfine
structure intervals 8 Delta nu_2 - Delta nu_1. Nuclear effects for are not
important this difference and it is of use to study higher-order QED
corrections.Comment: 10 pages, presented at Hydrogen Atom II meeting (2000
One-loop self-energy correction to the 1s and 2s hyperfine splitting in H-like systems
The one-loop self-energy correction to the hyperfine splitting of the 1s and
2s levels in H-like low-Z atoms is evaluated to all orders in Z\alpha. The
results are compared to perturbative calculations. The residual higher-order
contribution is evaluated. Implications to the specific difference of the
hyperfine structure intervals 8\Delta \nu_2 - \Delta \nu_1 in He^+ are
investigated.Comment: 17 pages, RevTeX, 3 figure
Intracellular Mass Density Increase Is Accompanying but Not Sufficient for Stiffening and Growth Arrest of Yeast Cells
Many organisms, including yeast cells, bacteria, nematodes, and tardigrades, endure harsh environmental conditions, such as nutrient scarcity, or lack of water and energy for a remarkably long time. The rescue programs that these organisms launch upon encountering these adverse conditions include reprogramming their metabolism in order to enter a quiescent or dormant state in a controlled fashion. Reprogramming coincides with changes in the macromolecular architecture and changes in the physical and mechanical properties of the cells. However, the cellular mechanisms underlying the physical-mechanical changes remain enigmatic. Here, we induce metabolic arrest of yeast cells by lowering their intracellular pH. We then determine the differences in the intracellular mass density and stiffness of active and metabolically arrested cells using optical diffraction tomography (ODT) and atomic force microscopy (AFM). We show that an increased intracellular mass density is associated with an increase in stiffness when the growth of yeast is arrested. However, increasing the intracellular mass density alone is not sufficient for maintenance of the growth-arrested state in yeast cells. Our data suggest that the cytoplasm of metabolically arrested yeast displays characteristics of a solid. Our findings constitute a bridge between the mechanical behavior of the cytoplasm and the physical and chemical mechanisms of metabolically arrested cells with the ultimate aim of understanding dormant organisms