DEVELOPMENT AND VALIDATION OF UV-VISIBLE SPECTROPHOTOMETRIC METHOD FOR ANALYSIS OF BOSENTAN IN SPIKED HUMAN PLASMA

Objective: The aim of the present study is to develop and validate a simple, efficient, economical and accurate UV-visible spectrophotometric method for estimation of bosentan in spiked human plasma. Methods: The analyte was extracted by Liquid-liquid Extraction (LLE) procedure using acetonitrile and chloroform. Absorbance of the analyte in the extract was measured at 270 nm using ethanol as a diluent. The developed method was validated for linearity, accuracy and robustness. Results: The proposed method was found to be linear in the range of 6 to 18 mg/ml. The correlation coefficient (r2) was found to be 0.99. The results revealed that the linearity, accuracy and robustness of the developed method were within the acceptable range. Conclusion: The analytical technique presented here demonstrates shorter and easier sample preparation method, decreased analysis time and reduces the need for complicated or expensive equipment. The sample preparation method used in this study can also be further extended to higherend analytical techniques and other biological samples for quantification of bosentan

Drug utilization study in post operative patients in surgical ward of tertiary care teaching hospital

Background: This study was aimed to evaluate the drug prescription pattern and to analyze the drug usage in post operative surgical patients with appendicitis, cellulitis, diabetic foot ulcer, hernia and hydrocele in tertiary care teaching hospital.Methods: A prospective observational study was conducted over a period of six months in post-operative surgical patients at Mahatma Gandhi Memorial Hospital, Warangal, Telangana, India, which was approved by Institutional Ethics Committee. The data obtained was analyzed using graph pad prism 7.0 and the conclusions were drawn using descriptive analysis.Results: A total of 429 cases were recruited during the following study period of which, parenteral drugs were pantoprazole 399 (93%), ceftriaxone 319 (74.35%), metronidazole 239 (55.71%). Oral drugs were multivitamin 400 (93.24%), serratiopeptidase 299 (69.69%), voveran 162 (37.76%). Majority of the drugs prescribed were intravenous fluids, antacids, antibiotics, analgesics were accounted. The percentage encounters with generic drugs was 99% in present study.Conclusions: The present study provides valuable insight about the overall pattern of drug use profile in postoperative patients in the surgical unit of a tertiary care hospital. The study is useful in decreasing the irrational prescription, which helps to decrease the morbidity and health care burden in the society. The prescribing pattern was according to the National Essential Drug List is very low and needs improvement

Effect of Soaking and Germination on Antinutritional Factors of Quinoa (Chenopodium quinoa)

Quinoa is a nutritional powerhouse, packed with protein, fiber, vitamins and minerals. However, it also contains certain compounds that impact nutrient availability. To address this, processing methods like soaking and germination have emerged as effective traditional treatments. They not only enhance the nutritional and bioactive potential but also diminish the anti-nutritional components in these grains, elevating their overall quality. This study examines the effect of soaking and germination treatments on anti-nutritional characteristics of quinoa. The result shows that an increase in germination percentages increases with longer germination times. Notably, seeds soaked in lukewarm water containing 2% salt for 24 hours showed the highest germination percentage (82.89%), while the number of non-germinated (16.27%) and abnormally germinated (0.32%) seeds decreased and greatest radical length (10.25 mm) when compared to seeds soaked in normal water. However, as the germination period extended, quinoa exhibited increased levels of vitamin C, notably more pronounced in lukewarm water with a 2% salt concentration. Additionally, germinated quinoa presents reduced levels of phytic acid (68.22 mg/100 g), tannin (0.36 mg/100 g), saponin (75.13 mg/100 g), and oxalates (42.55 mg/100 g) in comparison to raw quinoa. Hence, soaking and germination emerge as effective methods for reducing antinutritional components and enhancing the nutritional and bioactive potential of quinoa