Allosteric collaboration between elongation factor G and the ribosomal L1 stalk directs tRNA movements during translation

Determining the mechanism by which transfer RNAs (tRNAs) rapidly and precisely transit through the ribosomal A, P and E sites during translation remains a major goal in the study of protein synthesis. Here, we report the real-time dynamics of the L1 stalk, a structural element of the large ribosomal subunit that is implicated in directing tRNA movements during translation. Within pre-translocation ribosomal complexes, the L1 stalk exists in a dynamic equilibrium between open and closed conformations. Binding of elongation factor G (EF-G) shifts this equilibrium towards the closed conformation through one of at least two distinct kinetic mechanisms, where the identity of the P-site tRNA dictates the kinetic route that is taken. Within post-translocation complexes, L1 stalk dynamics are dependent on the presence and identity of the E-site tRNA. Collectively, our data demonstrate that EF-G and the L1 stalk allosterically collaborate to direct tRNA translocation from the P to the E sites, and suggest a model for the release of E-site tRNA

Molecular wires acting as quantum heat ratchets

We explore heat transfer in molecular junctions between two leads in the absence of a finite net thermal bias. The application of an unbiased, time-periodic temperature modulation of the leads entails a dynamical breaking of reflection symmetry, such that a directed heat current may emerge (ratchet effect). In particular, we consider two cases of adiabatically slow driving, namely (i) periodic temperature modulation of only one lead and (ii) temperature modulation of both leads with an ac driving that contains a second harmonic, thus generating harmonic mixing. Both scenarios yield sizeable directed heat currents which should be detectable with present techniques. Adding a static thermal bias, allows one to compute the heat current-thermal load characteristics which includes the ratchet effect of negative thermal bias with positive-valued heat flow against the thermal bias, up to the thermal stop-load. The ratchet heat flow in turn generates also an electric current. An applied electric stop-voltage, yielding effective zero electric current flow, then mimics a solely heat-ratchet-induced thermopower (``ratchet Seebeck effect''), although no net thermal bias is acting. Moreover, we find that the relative phase between the two harmonics in scenario (ii) enables steering the net heat current into a direction of choice.Comment: 9 pages, 8 figure

A framework for evaluating automatic image annotation algorithms

Several Automatic Image Annotation (AIA) algorithms have been introduced recently, which have been found to outperform previous models. However, each one of them has been evaluated using either different descriptors, collections or parts of collections, or "easy" settings. This fact renders their results non-comparable, while we show that collection-specific properties are responsible for the high reported performance measures, and not the actual models. In this paper we introduce a framework for the evaluation of image annotation models, which we use to evaluate two state-of-the-art AIA algorithms. Our findings reveal that a simple Support Vector Machine (SVM) approach using Global MPEG-7 Features outperforms state-of-the-art AIA models across several collection settings. It seems that these models heavily depend on the set of features and the data used, while it is easy to exploit collection-specific properties, such as tag popularity especially in the commonly used Corel 5K dataset and still achieve good performance

Construction of a polarization insensitive lens from a quasi-isotropic metamaterial slab

We propose to employ the quasiisotropic metamaterial (QIMM) slab to construct a polarization insensitive lens, in which both E- and H-polarized waves exhibit the same refocusing effect. For shallow incident angles, the QIMM slab will provide some degree of refocusing in the same manner as an isotropic negative index material. The refocusing effect allows us to introduce the ideas of paraxial beam focusing and phase compensation by the QIMM slab. On the basis of angular spectrum representation, a formalism describing paraxial beams propagating through a QIMM slab is presented. Because of the negative phase velocity in the QIMM slab, the inverse Gouy phase shift and the negative Rayleigh length of paraxial Gaussian beam are proposed. We find that the phase difference caused by the Gouy phase shift in vacuum can be compensated by that caused by the inverse Gouy phase shift in the QIMM slab. If certain matching conditions are satisfied, the intensity and phase distributions at object plane can be completely reconstructed at image plane. Our simulation results show that the superlensing effect with subwavelength image resolution could be achieved in the form of a QIMM slab.Comment: 25 pages, 8 figure

Intermediate Wakimoto modules for Affine sl(n+1)

We construct certain boson type realizations of affine sl(n+1) that depend on a parameter r. When r=0 we get a Fock space realization of Imaginary Verma modules appearing in the work of the first author and when r=n they are the Wakimoto modules described in the work of Feigin and Frenkel

Controlling Excitations Inversion of a Cooper Pair Box Interacting with a Nanomechanical Resonator

We investigate the action of time dependent detunings upon the excitation inversion of a Cooper pair box interacting with a nanomechanical resonator. The method employs the Jaynes-Cummings model with damping, assuming different decay rates of the Cooper pair box and various fixed and t-dependent detunings. It is shown that while the presence of damping plus constant detunings destroy the collapse/revival effects, convenient choices of time dependent detunings allow one to reconstruct such events in a perfect way. It is also shown that the mean excitation of the nanomechanical resonator is more robust against damping of the Cooper pair box for convenient values of t-dependent detunings.Comment: 11 pages, 5 figure

Influence of traditional ecological knowledge on conservation of the skywalker hoolock gibbon (Hoolock tianxing) outside nature reserves

Although many species are threatened by hunting or resource extraction from indigenous human communities, traditional ecological knowledge (TEK) of local communities has the potential to support management and conservation of natural resources and wildlife. The newly described skywalker hoolock gibbon (Hoolock tianxing) is found on the border of China and Myanmar, and a large proportion of the remaining population in China occurs outside nature reserves. We surveyed this species across its range in China, and interviewed 622 people in 99 villages to evaluate the relationship between gibbon status and TEK of local communities. The total confirmed population was estimated to be less than 150 individuals. Gibbon subpopulations appear to have remained stable from 2009 to 2017 both within and outside nature reserves. Sociological and environmental correlates of gibbon survival outside the reserve were: (1) more Lisu than Han people present in villages; (2) greater forest cover; (3) greater distance from county towns; (4) existence of traditional taboos on hunting gibbons; and (5) higher dependency on forest resources by villagers. Interviewees living closer to surviving gibbon populations were more knowledgeable about gibbons, although interviewees living more than 25 km away also knew more about gibbons. Formal education level was also correlated with better knowledge of gibbons, and men were better informed about gibbons than women. TEK appears to limit poaching of gibbons, thus contributing to their survival. The persistence of gibbons outside nature reserves may depend on incorporating TEK within community-based conservation strategies

An Exactly Solvable Model of Generalized Spin Ladder

A detailed study of an $S={1\over2}$ spin ladder model is given. The ladder consists of plaquettes formed by nearest neighbor rungs with all possible SU(2)-invariant interactions. For properly chosen coupling constants, the model is shown to be integrable in the sense that the quantum Yang-Baxter equation holds and one has an infinite number of conserved quantities. The R-matrix and L-operator associated with the model Hamiltonian are given in a limiting case. It is shown that after a simple transformation, the model can be solved via a Bethe ansatz. The phase diagram of the ground state is exactly derived using the Bethe ansatz equation