Porównanie zawartości pierwiastków w roślinach fasoli szparagowej uprawianych w systemie organicznym i konwencjonalnym w celach spożywczych i leczniczych

Today, sustainable agriculture and food content is a very important issue in the world. Organic farming practices are very important in this respect.This study was made to observe the impact of organic agricultural regime on the products in case of haricot beans (Phaseolus vulgaris L.). A comparative study on elemental composition of various haricot beans (Phaseolus vulgaris L.) samples was conducted by using a sensitive method, wavelength dispersive X-ray fluorescence (WDXRF). 26 elements such as Al, Ca, Cu, Fe, As, Hg, Pb, Cd, Bi, Mn, Ni, P, S, Sr, Zn, Zr, La, Ti, Sn, Cl, K, Mg, Na, Ba, Rb and Si were determined in haricot beans samples (n=10) grown under organic and conventional farming regimes. The obtained results from each group were analyzed statistically by using SPSS statistic program. It was observed that the concentration and peak intensity values of Ca, Fe, Mn, P, Zn, Cl, K, Na, Mg and Si elements were higher in the haricot beans samples grown under organic farming regime. Likewise, Al and Sr levels were found in higher levels in the samples grown under conventional farming regime. Our findings clearly revealed that organic haricot beans are likely to have higher nutritional mineral content. And the haricot beans samples grown under conventional farming regime could contain harmful metals like Al and Sr that might damage the various systems and/or organs of humans and animals.Obecnie rolnictwo nienaruszające równowagi ekologicznej oraz wartość żywności stanowią sprawę istotną dla całego świata. Praktyki uprawy ekologicznej mają w tej kwestii duże znaczenie. Celem niniejszej pracy jest obserwacja wpływu systemu rolnictwa organicznego na produkty, na przykładzie fasoli szparagowej (Phaseolus vulgaris L.). Przeprowadzono badanie porównawcze składu pierwiastkowego (chemicznego) różnych próbek fasoli szparagowej (Phaseolus vulgaris L.) czułą metodą fluorescencji rozpraszającej długość fali promieniami Roentgena (WDXRF). 26 pierwiastków, takich jak: Al, Ca, Cu, Fe, As, Hg, Pb, Cd, Bi, Mn, Ni, P, S, Sr, Zn, Zr, La, Ti, Sn, Cl, K, Mg, Na, Ba, Rb oraz Si oznaczono w próbkach fasoli szparagowej (n = 10) uprawianej systemem organicznym i tradycyjnym. Otrzymane wyniki z każdej grupy poddano analizie statystycznej z wykorzystaniem programu statystycznego SPSS. Zaobserwowano, że wartości stężenia I szczytowej intensywności pierwiastków Ca, Fe, Mn, P, Zn, Cl, K, Na, Mg oraz Si były wyższe w próbkach fasoli szparagowej uprawianej systemem organicznym. Stwierdzono także wyższy poziom Al i Sr w próbkach uprawianych zgodnie z systemem konwencjonalnym. Nasze wyniki wyraźnie wykazały, że uprawiana organicznie fasola szparagowa prawdopodobnie zawiera więcej mineralnych składników odżywczych, zaś uprawiana zgodnie z systemem konwencjonalnym może zawierać szkodliwe metale, takie jak Al i Sr, mogące uszkadzać różne narządy i/lub układy w organizmach ludzi i zwierząt

An antidote for imazalil-induced genotoxicity in vitro: The lichen, Dermatocarpon intestiniforme (Körber) hasse

Imazalil (IMA), a commonly used fungicide in both agricultural and clinical domains, is suspected to produce serious toxic effects in vertebrates. In recent years, a number of studies have suggested that lichens might be easily accessible sources of natural drugs that could be used as a possible food supplement. Extensive research is being performed to explore the importance of lichen species, which are known to contain a variety of pharmacological active compounds. In this context, the antigenotoxic effect of aqueous Dermatocarpon intestiniforme (Körber) Hasse. extract (DIE) was studied against the genotoxic damage induced by IMA on cultured human lymphocytes (n = 6) using chromosomal aberration (CA) and micronucleus (MN) as cytogenetic endpoints. Human peripheral lymphocytes were treated in vitro with varying concentrations of DIE (0, 25, 50 and 100 μg/ml), tested in combination with IMA (336 μg/ml). DIE alone were not genotoxic and when combined with IMA treatment, it reduced the frequency of CAs and the rate of MNs. A clear dose-dependent decrease in the genotoxic damage of IMA was observed, suggesting a genoprotective role of DIE. The results of the present study suggest that this plant extract per se does not have a genotoxic potential, but can alleviate the genotoxicity of IMA on cultured human lymphocytes. In conclusion our findings may have an important application for the protection of cultured human lymphocyte from the genetic damage and side effects induced by medical and agricultural chemicals hazardous for people

Evaluation of antioxidant and cytotoxic effects of olivetoric and physodic acid in cultured human amnion fibroblasts

WOS:000400102100006PubMed:27206701It is known that lichens are utilized for the treatment of many diseases including ulcer, diabetes, and cancer for many years. Secondary metabolites in the structure of the lichens provide various activity properties for them. In the present study, cytotoxic and oxidative effects of main constituents of Pseudevernia furfuracea (L.) Zopf (Parmeliaceae), olivetoric acid (OA), and physodic acid (PA) were investigated on cultured human amnion fibroblasts (HAFs). OA and PA were isolated from P. furfuracea using column chromatography and their structures were determined by proton nuclear magnetic resonance and carbon-13 nuclear magnetic resonance. HAFs were incubated during 48 h in the presence of OA and PA, at different concentrations from 6.25 mg/L to 200 mg/L. OA showed higher cytotoxicity than PA. In fact, median inhibitory concentration values of OA and PA were 571.27 and 3373.69 mg/L, respectively. The lower concentrations (<50 mg/L) of OA and PA did not cause oxidative stress and genotoxicity; furthermore, they supported anti-oxidative capacity of HAFs. Therefore, all these data suggested that both tested metabolites, especially PA might be developed as natural health medicine to protect human body against oxidative stress and genotoxicity. As far as we know, this is the first report on the cytotoxic and anti-oxidative activities of OA and PA on HAFs

Inhibition of growth of U87MG human glioblastoma cells by Usnea longissima ach

PubMed:31596391Herbal medicines are efficient to reduce side effects in the fight against glioblastoma, which plays a critical role within brain cancer species. The recent studies designated for testing the effects of lichens that have shown numerous anticancer activities on glioblastoma so far. In the present study, different concentrations of water extract obtained from Usnea longissima Ach. were used in order to determine cytotoxic (via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and lactate dehydrogenase tests), antioxidant (via total antioxidant capacity test), pro-oxidant (via total oxidant status test) and genotoxic (via 8-hydroxy-2?-deoxyguanosine test) effects of them on human U87MG-glioblastoma cancer cell lines. Primary mixed glial-neuronal non-cancerous cells from Sprague-Dawley rats were also utilized to measure the effects of treatments on non-cancerous cells. Based on median inhibitory concentration values, the data belonged to non-cancerous cells (2486.71 mg/L) showed distinct towering compared to U87MG (80.93 mg/L) cells. The viability of non-cancerous and U87MG cells exposed to extract is decreased in a dose dependent manner. It was also showed that low concentrations of extract notably increased total antioxidant capacity on non-cancerous cells. In addition, various phenolic compounds in extract were detected through high-performance liquid chromatography. The recent results encourage that extract will be able to have therapeutic potential against glioblastoma

Anticancer effects of novel NSAIDs derivatives on cultured human glioblastoma cells

WOS:000672580800007PubMed ID:32889798Several epidemiologic, clinical and experimental reports indicate that nonsteroidal anti-inflammatory drugs (NSAIDs) could have a potential as anticancer agents. The aim of this study was the evaluation of cytotoxic potential in human glioblastoma cells of novel synthesized NSAID derivatives, obtained by linking, through a spacer, ?-lipoic acid (ALA) to anti-inflammatory drugs, such as naproxen (AL-3, 11 and 17), flurbiprofen (AL-6, 13 and 19) and ibuprofen (AL-9, 15 and 21). The effects on the level of gene expression were also determined using quantitative real-time polymerase chain reaction (qRT-PCR) analysis. According to our results, NSAID derivatives exhibited concentration dependent cytotoxic effects on U87-MG cell line when compared with the control group. Moreover, treatment of the most active compounds (AL-3, AL-6 and AL-9) caused upregulation of tumor suppressor gene PTEN and downregulation of some oncogenes such as AKT1, RAF1 and EGFR. In conclusion, our results revealed that AL-3, AL-6 and AL-9 could be suitable candidates for further investigation to develop new pharmacological strategies for the prevention of cancer. © 2020 Walter de Gruyter GmbH, Berlin/Boston 2020.2015-12, 2015-12, 2015-12, 2015-12 2015-12, 2015-12, 2015-12, 2015-12This study was supported by Erzurum Technical University with the grant 2015-12.Research funding : This study was supported by Erzurum Technical University with the grant 2015-12