Recovery of in-situ methanotrophic activity following acetylene inhibition

Abstract Methane (CH 4 ) is the second most important greenhouse gas after carbon dioxide (CO 2 ). To understand CH 4 cycling, quantitative information about microbial CH 4 oxidation in soils is essential. Field methods such as the gas push-pull test (GPPT) to quantify CH 4 oxidation are often used in combination with specific inhibitors, such as acetylene (C 2 H 2 ). Acetylene irreversibly binds to the enzyme methane monooxygenase, but little is known about recovery of CH 4 oxidation activity after C 2 H 2 inhibition in situ, which is important when performing several experiments at the same location. To assess recovery of CH 4 oxidation activity following C 2 H 2 inhibition, we performed a series of GPPTs over 8 weeks at two different locations in the vadose zone above a petroleum hydrocarbon-contaminated aquifer in Studen, Switzerland. After 4 weeks a maximum recovery of 30% and 50% of the respective initial activity was reached, with a subsequent slight drop in activity at both locations. Likely, CH 4 oxidation activity and CH 4 concentrations were too low to allow for rapid recovery following C 2 H 2 inhibition at the studied locations. Therefore, alternative competitive inhibitors have to be evaluated for application in conjunction with GPPTs, especially for sites with low activity

Biophysical interactions in tropical agroforestry systems

sequential systems, simultaneous systems Abstract. The rate and extent to which biophysical resources are captured and utilized by the components of an agroforestry system are determined by the nature and intensity of interac-tions between the components. The net effect of these interactions is often determined by the influence of the tree component on the other component(s) and/or on the overall system, and is expressed in terms of such quantifiable responses as soil fertility changes, microclimate modification, resource (water, nutrients, and light) availability and utilization, pest and disease incidence, and allelopathy. The paper reviews such manifestations of biophysical interactions in major simultaneous (e.g., hedgerow intercropping and trees on croplands) and sequential (e.g., planted tree fallows) agroforestry systems. In hedgerow intercropping (HI), the hedge/crop interactions are dominated by soil fertility improvement and competition for growth resources. Higher crop yields in HI than in sole cropping are noted mostly in inherently fertile soils in humid and subhumid tropics, and are caused by large fertility improvement relative to the effects of competition. But, yield increases are rare in semiarid tropics and infertile acid soils because fertility improvement does not offse

Trees improve water storage and reduce soil evaporation in agroforestry systems on bench terraces in SW Uganda

The success of agroforestry in semi-arid areas depends on efficient use of available water and effective strategies to limit tree/crop competition and maximise productivity. On hillsides, planting improved tree fallows on the degraded upper section of bench terraces is a recommended practice to improve soil fertility while cropping continues on the lower terrace to maintain food production. This study examined the influence of tree fallows on soil water content (θ w ) and evaporation (E s ). Alnus acuminata Kunth (alnus), Calliandra calothyrsus Meissner (calliandra), Sesbania sesban L. (sesbania), a mixture of all three species, or sole crops (beans (Phaseolus vulgaris L.) or maize (Zea mays L.)) were grown on the upper terrace. The same sole crops were grown on the lower terrace. Four management regimes (unpruned, root, shoot and root + shoot pruned) were applied to the tree rows adjacent to the cropping area. Neutron probe and microlysimeter approaches were used to determine θ w and E s when the trees were c. 3.5 years old. Sesbania and alnus increased θ w by 9–18 % in the cropping area on the lower terrace but calliandra reduced θ w by 3–15 %. After heavy rain, E s comprised 29–38 % of precipitation in the tree-based treatments and 53 % under sole crops. Absolute values declined as rainfall decreased, but E s as a proportion of rainfall increased to 39–45 % in the tree-based treatments and 62 % for sole crops. Root + shoot pruning of alnus and the tree mixture increased θ w in the cropping area but had no significant effect in the other tree-based treatments. The results suggest that sesbania and alnus can be planted on smallholdings without compromising water supply to adjacent crops, whereas calliandra decreased water availability despite reducing E s . These results provide a mechanistic understanding of reported effects on crop yield in the same site

Diagrams for embeddings of polygons

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