Effects of rubber aggregates on the physical-mechanical, thermal and durability properties of self-compacting sand concrete

The aim of this research was to study the effect of incorporating waste rubber aggregates on the physical, mechanical, thermal and durability performance of Self-Compacting Sand Concrete SCSC mixtures. For this purpose, the separately developed Rubberized Self-Compacting Sand Concrete RSCSC were prepared with three fractions of rubber grains where the natural aggregates were replaced with powder rubber, sand rubber and gravel rubber and four addition ratios (5, 10, 15 and 20%) as volume rates. The performed fresh properties using slump-flow, spreading, t500, sieve stability and air-entrained content tests proved better results for the RSCSC in comparison with reference concretes. Hardened state characterization of the concretes exhibited decreases in the mechanical properties of the RSCSC but the thermal conductivity and the dynamic elastic modulus were improved. Assessment of the concrete’s durability was accomplished through determination of apparent porosity, capillary absorption. Therefore, RSCSC to be can used in structural elements of dense reinforcement and complex formwork. Furthermore, this allows promising solution to reduce the impact of waste tyres on the environment and fight pollution

Maf1, a New Player in the Regulation of Human RNA Polymerase III Transcription

BACKGROUND: Human RNA polymerase III (pol III) transcription is regulated by several factors, including the tumor suppressors P53 and Rb, and the proto-oncogene c-Myc. In yeast, which lacks these proteins, a central regulator of pol III transcription, called Maf1, has been described. Maf1 is required for repression of pol III transcription in response to several signal transduction pathways and is broadly conserved in eukaryotes. METHODOLOGY/PRINCIPAL FINDINGS: We show that human endogenous Maf1 can be co-immunoprecipitated with pol III and associates in vitro with two pol III subunits, the largest subunit RPC1 and the α-like subunit RPAC2. Maf1 represses pol III transcription in vitro and in vivo and is required for maximal pol III repression after exposure to MMS or rapamycin, treatments that both lead to Maf1 dephosphorylation. CONCLUSIONS/SIGNIFICANCE: These data suggest that Maf1 is a major regulator of pol III transcription in human cells

Diversité dans l’administration fédérale: l’emploi des étrangers et des personnes d’origine étrangère dans la fonction publique fédérale

Rapport finalinfo:eu-repo/semantics/publishe

Des jeunes filles Maghrébines et Turques au carrefour de deux systèmes de valeurs en Belgique

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Enaminones in a multicomponent synthesis of 4-aryldihydropyridines for potential applications in photoinduced intramolecular electron-transfer systems

Abstract An efficient three component reaction with enaminones, primary amines and aldehydes resulted in easy access to 1,4-dihydropyridines with different substituents at the 1-, 3-, 4-and 5-positions. Microwaves improved the reaction yield, reducing also considerably the reaction time and the amount of solvent used. Chiral primary amines gave chiral 1-substituted-1,4-dihydropyridines. The 4-(1-naphthyl) and 4-(phenanthren-9-yl)dihydropyridine derivatives exhibited an interesting photoluminescence behavior, which suggests their potential application as suitable photoinduced intramolecular electron-transfer systems. 44

Diversité dans l'administration fédérale: l'emploi des étrangers et des personnes d'origine étrangère dans la focntion publique fédérale :rapport final

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Defining the RNA polymerase III transcriptome: Genome-wide localization of the RNA polymerase III transcription machinery in human cells

Our view of the RNA polymerase III (Pol III) transcription machinery in mammalian cells arises mostly from studies of the RN5S (5S) gene, the Ad2 VAI gene, and the RNU6 (U6) gene, as paradigms for genes with type 1, 2, and 3 promoters. Recruitment of Pol III onto these genes requires prior binding of well-characterized transcription factors. Technical limitations in dealing with repeated genomic units, typically found at mammalian Pol III genes, have so far hampered genome-wide studies of the Pol III transcription machinery and transcriptome. We have localized, genome-wide, Pol III and some of its transcription factors. Our results reveal broad usage of the known Pol III transcription machinery and define a minimal Pol III transcriptome in dividing IMR90hTert fibroblasts. This transcriptome consists of some 500 actively transcribed genes including a few dozen candidate novel genes, of which we confirmed nine as Pol III transcription units by additional methods. It does not contain any of the microRNA genes previously described as transcribed by Pol III, but reveals two other microRNA genes, MIR886 (hsa-mir-886) and MIR1975 (RNY5, hY5, hsa-mir-1975), which are genuine Pol III transcription units

Maf1 represses transcription from type I, II, and III pol III promoters <i>in vitro.</i>

<p>A) Maf1 represses transcription from the 5S, VAI, and 7SL pol III promoters, but not the Ad2 ML pol II promoter, <i>in vitro</i>. 40, 80, 400, and 800 ng of bacterially produced Maf1 (lanes 3–6) or similar amounts of Brf2 (lanes 7–10) were added to transcription reactions identical to that shown in lane 2. Lane 1 shows unprogrammed transcription extract. B) SDS-polyacrylamide gel stained with coomassie blue indicating the amounts of recombinant Maf1 and Brf2 used in the <i>in vitro</i> transcription experiment described in A. C) Maf1 represses transcription from the U6 promoter <i>in vitro</i>. The lanes are as in A, except that the HeLa cell extract was programmed with the U6 promoter and that increasing amounts of GST rather than Brf2 were added as control. D) SDS-polyacrylamide gel stained with coomassie blue indicating the amounts of recombinant Maf1 and GST used in the <i>in vitro</i> transcription experiment described in C. E) Maf1 associates with pol III in the transcription extract. <i>In vitro</i> transcription reactions containing either no (lane 2) or 400 ng (lane 3) of recombinant His-tagged Maf1 were incubated with Ni-NTA beads, and the beads were then washed several times with D100 buffer <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0000134#pone.0000134-Dignam1" target="_blank">[40]</a>. The affinity-purified complex was analysed by SDS-PAGE followed by immunoblotting with anti-RPC1 or anti-His tag antibodies.</p

Human Maf1 is phosphorylated and becomes dephosphorylated after rapamycin and MMS treatment.

<p>A) Nuclear extract from a HEK 293 cell line expressing HA-tagged Maf1 either untreated (upper panel), or treated with rapamycin (middle panel) or MMS (lower panel) was incubated with nothing, CIAP with or without phosphatase inhibitors, or phosphatase inhibitors alone, as indicated above the lanes. Tagged Maf1 was visualized with an antibody directed against the HA tag. Lane 5 shows bacterially expressed Maf1, which was loaded on a non-adjacent lane of the same gel. B) Nuclear extract from IMR-90Tert cells transiently expressing HA-tagged Maf1 was prepared in the presence of phosphatase inhibitors prior to immunoprecipitation with affinity-purified anti-RPC1 antibody. Tagged Maf1 was visualized with an antibody directed against the tag (α-HA antibody). Only the fastest migrating form of Maf1 bound to pol III. Arrows: phosphorylated Maf1. The negative control was a mock immunoprecipitation performed with protein A sepharose beads without antibody. Lane 1 shows 1/20 of the input material.</p