Chromosome polymorphism in Bulgarian populations of the striped field mouse (Apodemus agrarius Pallas 1771)

Chromosome polymorphism in Bulgarian populations of the striped field mouse (Apodemus agrarius Pallas, 1771) has been described. The diploid chromosome number is 2n=48 (NFa = 54). In the karyotype of 3 specimens from the Iskar region, the presence of an additional B chromosome has been established for the first time. The autosomes are 19 acrocentric pairs, continuously decreasing in size, and 4 pairs of bi-armed chromosomes, barely distinguishable by size and location of the centromere. Specimens with 3 pairs of metacentric chromosomes were firstly described in Bulgaria for the regions of Iskar and Omurtag. The localization of heterochromatin in the centromeric regions of the chromosomes, blocks of heterochromatin of different sizes, as well as intercalated bands, distinguishable in weakly spiraled chromosomes are found. Telomeric heterochromatin is present in the largest autosomal pair and in two of the middle-sized autosomal pairs. The largest and smallest pairs of be-armed chromosomes do not have centromeric heterochromatin, whereas all the other autosomal pairs do. The presence of a NOR in 6 chromosomal pairs is established. Two of the pairs exhibited pericentromeric NORs, whereas the other 4 displayed telomeric NORs. The karyotype analysis illustrates the chromosome and genome polymorphism of A. agrarius in Bulgarian populations

Chromosome polymorphism in Bulgarian populations of the striped field mouse (Apodemus agrarius Pallas 1771)

Chromosome polymorphism in Bulgarian populations of the striped field mouse (Apodemus agrarius Pallas, 1771) has been described. The diploid chromosome number is 2n=48 (NFa = 54). In the karyotype of 3 specimens from the Iskar region, the presence of an additional B chromosome has been established for the first time. The autosomes are 19 acrocentric pairs, continuously decreasing in size, and 4 pairs of bi-armed chromosomes, barely distinguishable by size and location of the centromere. Specimens with 3 pairs of metacentric chromosomes were firstly described in Bulgaria for the regions of Iskar and Omurtag. The localization of heterochromatin in the centromeric regions of the chromosomes, blocks of heterochromatin of different sizes, as well as intercalated bands, distinguishable in weakly spiraled chromosomes are found. Telomeric heterochromatin is present in the largest autosomal pair and in two of the middle-sized autosomal pairs. The largest and smallest pairs of be-armed chromosomes do not have centromeric heterochromatin, whereas all the other autosomal pairs do. The presence of a NOR in 6 chromosomal pairs is established. Two of the pairs exhibited pericentromeric NORs, whereas the other 4 displayed telomeric NORs. The karyotype analysis illustrates the chromosome and genome polymorphism of A. agrarius in Bulgarian populations

Verfahren zur Herstellung einer permanenten Entformungsschicht durch Plasmapolymerisation auf der Oberflaeche eines Formteilwerkzeugs, ein nach dem Verfahren herstellbares Formteilwerkzeug und dessen Verwendung

WO 200205972 A UPAB: 20020613 NOVELTY - By temporal variation of polymerization conditions, a release layer with property gradient is produced. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is included for the corresponding mold with permanent layer of parting agent. USE - To make a permanent mold release layer with property gradient. The mold is used to produce plastic objects, e.g. from polyurethane or polyurethane foam (claimed applications). ADVANTAGE - A low-energy, actively-separating surface is formed, which is so stable that it may be considered permanent. A valuable feature is the clean surface produced on the molding. It requires no additional cleaning stage before e.g. adhering, lining, painting or metallizing onto the molding. The layer resists temperatures at least up to 200 deg. C and can be cleaned with a little solvent, e.g. benzene or isopropanol on a cloth, without damage. The layer remains effective even after some mechanical damage. Even after considerable damage, it is merely necessary to after-coat the mold

Current progress and future opportunities in applications of bioinformatics for biodefense and pathogen detection: report from the Winter Mid-Atlantic Microbiome Meet-up, College Park, MD, January 10, 2018

Abstract The Mid-Atlantic Microbiome Meet-up (M3) organization brings together academic, government, and industry groups to share ideas and develop best practices for microbiome research. In January of 2018, M3 held its fourth meeting, which focused on recent advances in biodefense, specifically those relating to infectious disease, and the use of metagenomic methods for pathogen detection. Presentations highlighted the utility of next-generation sequencing technologies for identifying and tracking microbial community members across space and time. However, they also stressed the current limitations of genomic approaches for biodefense, including insufficient sensitivity to detect low-abundance pathogens and the inability to quantify viable organisms. Participants discussed ways in which the community can improve software usability and shared new computational tools for metagenomic processing, assembly, annotation, and visualization. Looking to the future, they identified the need for better bioinformatics toolkits for longitudinal analyses, improved sample processing approaches for characterizing viruses and fungi, and more consistent maintenance of database resources. Finally, they addressed the necessity of improving data standards to incentivize data sharing. Here, we summarize the presentations and discussions from the meeting, identifying the areas where microbiome analyses have improved our ability to detect and manage biological threats and infectious disease, as well as gaps of knowledge in the field that require future funding and focus