Requirement of the FATC domain of protein kinase Tel1 for localization to DNA ends and target protein recognition

Two large phosphatidylinositol 3-kinase-related protein kinases (PIKKs), ATM and ATR, play a central role in the DNA damage response pathway. PIKKs contain a highly conserved extreme C-terminus called the FRAP-ATM-TRRAP-C-terminal (FATC) domain. In budding yeast, ATM and ATR correspond to Tel1 and Mec1, respectively. In this study, we characterized functions of the FATC domain of Tel1 by introducing substitution or truncation mutations. One substitution mutation, termed tel1-21, and a truncation mutation, called tel1- Î"C, did not significantly affect the expression level. The tel1-21 mutation impaired the cellular response to DNA damage and conferred moderate telomere maintenance defect. In contrast, the tel1-Î"C mutation behaved like a null mutation, conferring defects in both DNA damage response and telomere maintenance. Tel1-21 protein localized to DNA ends as effectively as wild-type Tel1 protein, whereas Tel1-Î"C protein failed. Introduction of a hyperactive TEL1-hy mutation suppressed the tel1-21 mutation but not the tel1-Î"C mutation. In vitro analyses revealed that both Tel1-21 and Tel1-Î"C proteins undergo efficient autophosphorylation but exhibit decreased kinase activities toward the exogenous substrate protein, Rad53. Our results show that the FATC domain of Tel1 mediates localization to DNA ends and contributes to phosphorylation of target proteins. © 2015 Ogi, Goto, Ghosh, et al

Lost equivalence of nonlinear sigma and CP1CP^{1} models on noncommutative space

We show that the equivalence of nonlinear sigma and $CP^{1}$ models which is valid on the commutative space is broken on the noncommutative space. This conclusion is arrived at through investigation of new BPS solitons that do not exist in the commutative limit.Comment: 17 pages, LaTeX2

Critical properties of the double exchange ferromagnet Nd0.4Pb0.4MnO3

Results of a study of dc-magnetization M(T, H), performed on a Nd0.6Pb0.4MnO3 single crystal in the temperature range around T_C (Curie temperature) which embraces the critical region | epsilon | = |T -T_C |/T_C <= 0.05 are reported. The magnetic data analyzed in the critical region using the Kouvel-Fisher method give the values for the T_C =156.47 +/- 0.06 K and the critical exponents, beta = 0.374 +/- 0.006 (from the temperature dependence of magnetization), and gamma = 1.329 +/- 0.003 (from the temperature dependence of initial susceptibility). The critical isotherm M(T_C, H) gives delta = 4.547 +/- 0.1. Thus the scaling law gamma+beta=delta beta is fulfilled. The critical exponents obey the single scaling-equation of state M(H, epsilon) = epsilon^b f_+/- (H/epsilon^(beta + gamma)) where, f_+ for T > T_C and f_- for T< T_C. The exponent values are very close to those expected for the universality class of 3D Heisenberg ferromagnets with short-range interactions.Comment: 19 pages, including 6 figure

Growth and physical property study of single nanowire (diameter ~ 45nm) of half doped Manganite

We report here the growth and characterization of functional oxide nanowire of hole doped manganite of La0.5Sr0.5MnO3 (LSMO). We also report four probe electrical resistance measurement of single nanowire of LSMO (diameter ~ 45nm) using FIB fabricated electrodes. The wires were fabricated by hydrothermal method using autoclave at a temperature of 270 oC. The elemental analysis and physical property like electrical resistivity were studied at individual nanowire level. The quantitative determination of Mn valency and elemental mapping of constituent elements was done by using Electron Energy Loss Spectroscopy (EELS) in the Scanning Transmission Electron Microscopy (STEM) mode. We addressed the important issue of whether as a result of size reduction the nanowires can retain the desired composition, structure and physical properties. The nanowires used were found to have a ferromagnetic transition (TC) at around 325 K which is very close to the bulk value of around 330 K found in single crystal of the same composition confirming that the functional behavior is likely to be retained even after size reduction of the nanowires to a diameter of 45 nm. The electrical resistivity shows insulating behavior within the temperature range measured, which is very much similar to the bulk system.Comment: 11 pages, 7 figures, accepted in Journal of Nanomaterial

Three flavour Quark matter in chiral colour dielectric model

We investigate the properties of quark matter at finite density and temperature using the nonlinear chiral extension of Colour Dielectric Model (CCM). Assuming that the square of the meson fields devlop non- zero vacuum expectation value, the thermodynamic potential for interacting three flavour matter has been calculated. It is found that $$and$$ remain zero in the medium whereas $$ changes in the medium. As a result, $u$ and $d$ quark masses decrease monotonically as the temperature and density of the quark matter is increased.In the present model, the deconfinement density and temperature is found to be lower compared to lattice results. We also study the behaviour of pressure and energy density above critical temperature.Comment: Latex file. 5 figures available on request. To appear in Phys. Rev.

Mapping of Large Scale 158 micron [CII] Line Emission: Orion A

We present the first results of an observational programme undertaken to map the fine structure line emission of singly ionized carbon ([CII] 157.7409 micron) over extended regions using a Fabry Perot spectrometer newly installed at the focal plane of a 100cm balloon-borne far-infrared telescope. This new combination of instruments has a velocity resolution of ~200 km/s and an angular resolution of 1.5'. During the first flight, an area of 30'x15' in Orion A was mapped. The observed [CII] intensity distribution has been compared with the velocity-integrated intensity distributions of 13CO(1-0), CI(1-0) and CO(3-2) from the literature. The observed line intensities and ratios have been analyzed using the PDR models by Kaufman et al. 1999 to derive the incident UV flux and volume density at a few selected positions.Comment: To appear in Astronomy & Astrophysic

Realization of Optimal Disentanglement by Teleportation via Separable Channel

We discuss here the best disentanglement processes of states of two two-level systems which belong to (i) the universal set, (ii) the set in which the states of one party lie on a single great circle of the Bloch sphere, and (iii) the set in which the states of one party commute with each other, by teleporting the states of one party (on which the disentangling machine is acting) through three particular type of separable channels, each of which is a mixture of Bell states. In the general scenario, by teleporting one party's state of an arbitrary entangled state of two two-level parties through some mixture of Bell states, we have shown that this entangled state can be made separable by using a physically realizable map $\tilde{V}$, acting on one party's states, if $\tilde{V} (I) = I, \tilde{V} ({\sigma}_j) = {\lambda}_j {\sigma}_j$, where ${\lambda}_j \ge 0$ (for $j = 1, 2, 3$), and ${\lambda}_1 + {\lambda}_2 + {\lambda}_3 \le 1$.Comment: 20 pages Late