52 research outputs found
CDK-Mediated Regulation of Cell Functions via c-Jun Phosphorylation and AP-1 Activation
Cyclin-dependent kinases (CDKs) and their targets have been primarily associated
with regulation of cell-cycle progression. Here we identify c-Jun, a
transcription factor involved in the regulation of a broad spectrum of cellular
functions, as a newly recognized CDK substrate. Using immune cells from mouse
and human, and several complementary in vitro and in
vivo approaches including dominant negative protein expression,
pharmacologic inhibitors, kinase assays and CDK4 deficient cells, we demonstrate
the ability of CDK4 to phosphorylate c-Jun. Additionally, the activity of AP-1,
a ubiquitous transcription factor containing phosphorylated c-Jun as a subunit,
was inhibited by abrogating CDK4. Surprisingly, the regulation of c-Jun
phosphorylation by CDK4 occurred in non-dividing cells, indicating that this
pathway is utilized for cell functions that are independent of proliferation.
Our studies identify a new substrate for CDK4 and suggest a mechanism by which
CDKs can regulate multiple cellular activation functions, not all of which are
directly associated with cell cycle progression. These findings point to
additional roles of CDKs in cell signaling and reveal potential implications for
therapeutic manipulations of this kinase pathway
The distribution of the apparent diffusion coefficient as an indicator of the response to chemotherapeutics in ovarian tumour xenografts
Cancer Council of Queensland and a mobility grant (Personalized Medicine) from the German Academic Exchange Service (DAAD)
The role of tissue microstructure and water exchange in biophysical modelling of diffusion in white matter
Editorial - Current advances in cancer research: Therapeutics, Targets, and Chemical Biology
Intrinsic Selectivity in Capillary Electrophoresis for Chiral Separations with Dual Cyclodextrin Systems
Hemodialysis increases apparent diffusion coefficient of brain water in nephrectomized rats measured by isotropic diffusion-weighted magnetic resonance imaging.
Solid-phase extraction of methadone enantiomers and benzodiazepines in biological fluids by two polymeric cartridges for liquid chromatographic analysis
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