Gastrointestinal stromal tumour as a rare association with neurofibromatosis type 1

Gastrointestinal stromal tumours (GIST) are rare tumours of mesenchymal origin. These can be associated with neurofibromatosis type 1 (NF1), which is an autosomal dominant disorder. The prevalence of GIST in NF1 is estimated at 3.9-25%. This paper describes the presentation of a GIST arising from the jejenum in a 75-year-old lady with NF1, who presented with gastrointestinal bleeding. This was diagnosed by CT angiography. She was managed with laparotomy, with resection of small bowel, and an ischaemic segment of large bowel with two primary anastomoses. Pathology showed GIST of spindle cell type (Figs 3 and 4), 90 mm in size, with complete local excision. The patient was discharged on the eighth post-operative day and is currently undergoing regular clinic follow-up after multidisciplinary team meeting discussion

The Sudan Peritoneal Dialysis Program: Three Years of Momentum

Introduction: End stage renal disease (ESRD) is a significant social and economic burden on Africa. Continuous ambulatory peritoneal dialysis (CAPD) was introduced as a national service in the Sudan three years ago. An overview of the Sudan Peritoneal Dialysis Program is presented. Review: As a national scheme, the Sudan PD program was sequentially launched in seven centers in Sudan. CAPD was the modality exclusively utilized. The total number of patients enrolled by July 2008 was 232. Patients' mean age was 43 years, 23.5% were children, 63% were males, and 19% were diabetic. PD was the initial dialysis modality for 54.7% of patients, 43.5% were referred from HD, and 1.9% had a failed kidney transplant. The majority of patients (62.3%) chose PD as the preferred dialysis modality, while the remainder were referred from HD to PD because of vascular access problems or hemodynamic instability. One third of patients required assisted PD, and 17% needed urgent initiation of dialysis. The cumulative peritonitis rate was one episode per 10.8 patient months. Touch contamination, urgent initiation of dialysis and execution of dialysis by family members other than trained helpers were among the identified predisposing factors. Conclusion: The establishment and operation of the Sudan PD program over the past three years have indicated that it is a promising project with multifaceted success. There is, however, an ongoing need for retraining of patients and staff. Early selection and careful preparation for potential PD candidates is also of vital importance to reduce the need for urgent dialysis initiation. Key words: peritoneal dialysis, CAPD, renal replacement therapy, Sudan, Afric

Renal Replacement Therapy Resources in Africa

Background: Africa is the world’s second-largest and most-populous continent. It is also the world’s poorest inhabited continent. Regarding chronic kidney disease (CKD), there are no reliable statistics in most African countries. However, there is a general impression that it is at least three to four times more frequent than in more developed countries Methods: a survey on renal replacement therapy in Africa was conducted in the context of the African Association of Nephrology (AFRAN) Congress 2007. A questionnaire was sent to leading African nephrologists, and data were also collected from the main dialysis supply companies and by personal communication. Data have been obtained from 32 out of 54 countries, representing 89% of the total population. Results: There are no reliable statistics regarding CKD in most African countries. The total number of nephrologists in the continent is 1154 (1-4 pmp). The total number of patients on hemodialysis (HD) is just over 60000 patients

Anti-inflammatory, anti-apoptotic, and antioxidant effects of obestatin on the colonic mucosa following acetic acid–induced colitis

Background: Cellular inflammatory processes, fibrogenesis, and apoptosis are the most characteristic pathologic features of colonic injury and colitis in human and experimental animals. Obestatin, a peptide derived from proghrelin, is reported to have significant protective and curative actions on many gastrointestinal tract inflammatory diseases, including ulcerative colitis (U.C.). However, its exact protective mechanisms and the associated histopathological changes, are still in need of deeper exploration. This study explores the effect of obestatin on the course of acetic acid (A.A.)-induced colitis as an antifibrotic, anti-inflammatory, and anti-apoptotic agent in relation to associated tissue stress parameters. Materials and methods: A total of 40 healthy male albino Wistar rats weighing 200–250 g were recruited in this study. The rats were classified into four groups (10 rats each); group I control, group II obestatin only treated (16 nmol/kg), group III, colitis induced group (acetic acid 1 mL of 3.5% (v/v), and group IV, (AA-induced colitis + obestatin) for 14 days. Colonic samples were examined after staining hematoxylin and eosin, Alcian blue, Masson trichrome. The expression of PCNA, NFkB, and Caspase-3 was estimated after immunohistochemical staining. Oxidative stress parameters, antioxidant enzymes, tissue myeloperoxidase (MPO) activity, ghrelin, and fibrogenesis markers were identified by immunoassay and colorimetric techniques. Results: Colonic mucosa of group IV exhibited mucosal healing and regeneration of the surface epithelium with the restoration of the goblet cells' function together with a decline in PCNA, NFkB, and Caspase-3 immunoreactivity in comparison to group III. This was accompanied by a reduction of the expression of fibrosis markers; Hydroxyproline and Fibronectin. In addition, tissue antioxidant status was significantly improved with a marked reduction of tissue MPO. Ghrelin level was significantly increased in comparison to group III. Group IV exhibited significant reduction in the levels of oxidative stress markers; MDA, TOS with a marked increase in the activity of antioxidant enzymes, SOD, CAT, and total cellular TAC. Conclusions: The concomitant treatment of obestatin inhibits the development of AA-induced colitis. The data signify that it has both curative and protective effects via antifibrotic, antioxidant, and anti-inflammatory activities

Embryoid induction and plantlet regeneration from leaf segments of sugarcane (Saccharum officinarum L.)

     Sugarcane (Saccharum officinarum L.) is an economically important crop in Sudan for domestic consumption and export. It is the first and essential source for production of high purity sugar. Tissue culture techniques can be used for in vitro conservation of sugarcane (Taylor and Dukic, 1993) and mass propagation of elite cultivars of crop species. Moreover, it was used for production of pathogen-free planting material from infected mother plants. Variability induced in vitro can furnish a base for improvement of vegetatively propagated crops including sugarcane. Different tissue culture techniques were applied successfully to sugarcane propagation and plant regeneration through organogenesis of shoot meristem (Nadar and Heinz, 1977; Ho and Vasil, 1983a), cell suspension cultures (Ho and Vasil, 1983b, Aftab et al., 1996) and protoplast cultures ( Liu, 1994). Cell suspen - Sion cultures were also used for cytological, pathological (Peros and Lombard, 1992), biochemical and physiological investigations of sugarcane (Heinz et al., 1977).      This study was initiated, during 1998-2000, to investigate the effect of 2,4-D on induction of embryogenic callus from leaf explants of sugarcane and regeneration of somatic embryos on different concen-trations of Murashige and Skoog (1962) medium (MS)

Use of Combined Economic Threshold Level to Control Insect Pests

The economic threshold level (ETL) is a key factor to be studied for insect pests control. It is difficult to monitor cotton insect pests separately, and it is not reasonable to base decision-making for spraying on an ETL of individual insects and ignore sub-levels of other cotton insect pests. So, we want to use a combined ETL in a way of insect units, to put all major insect pests to consideration as an insect pests‘ complex. This means delaying the first spray to give a chance for natural enemies to develop and then lower the number of sprays and consequently reduce the cost of production

Easily measureable morpho-physiological traits as selection criteria for terminal drought tolerance in groundnut (Arachis hypogaea L.)

This study was conducted at El Obied Research Station Farm, North Kordofan State Sudan, with the objective of identifiying easily measurable morpho-physiolgical traits that could be used in drought tolerance breeding programs. Nine groundnut mutants together with three parents were evaluated under normal and terminal drought stress environments in 2003 and 2004 cropping seasons in a randomized complete block design with four replications. The genotypes did not differ significantly in the number of days to 75% emergence, with  a range of 6-8 days. Most of the measured traits showed higher values under normal than under stressed environments. Under stressed environment, some mutants like Barberton-B-30-3 and Barberton-B-30-4, exceeded their parents in pod yield (PY) /plant, dry matter production (DM) and leaf relative water content (LRWC), whereas they showed lower specific leaf area (SLA), canopy temperature (CT) and leaf senescence (LSENS) than their parents. CT, LRWC, LSENS and PY showed relatively medium broad-sense heritab-ility estimates under stress environment. PY was positively, significantly and genotypically correlated with DM and LRWC while significantly and negatively correlated with SLA, CT and LSENS under stress enviro-nment. Since these traits are reasonably heritable, strongly correlated with PY under stress enviroment and easily measurable under field conditions, they could be suggested as selection criteria for droguht tolerance in groundnut. The mutant Barberton-B-30-3, which produced the highest PY, DM and LRWC, under terminal drought stress, could be suggested as the best drought tolerant mutant in this study bending further testing over years and locations. &nbsp

Chronic Kidney Disease in Police Forces Households in Khartoum, Sudan: Pilot Report

Introduction: In the Police Forces Hypertension, Diabetes, Renal Insufficiency and Thyroid Derangement (HyDRIT) pilot study we explored the prevalence, risk factors, awareness, treatment adequacy and complications of chronic kidney disease (CKD) and other non-communicable diseases among adult Police Forces households in two housing complexes in Khartoum, Sudan. Methods: Serum creatinine measurements and urine dipstick testing were performed for 273 individuals. The glomerular filtration rate (GFR) was estimated using the standardized Cockroft-Gault and the four variable MDRD formulas. CKD was defined as an estimated GFR of less than 60 ml/min/1.73m2 and/or proteinuria. Results: The overall prevalence of CKD was 11% (30/273) using the standardized Cockroft-Gault equation and 7.7% (21/273) using the four variable MDRD equation. According to the standardized Cockroft-Gault formula, 14 subjects had reduced GFR with negative urine dipstick testing, two subjects had both proteinuria and reduced GFR, and 14 subjects had proteinuria without reduction in GFR. None of those subjects was aware of his kidney problem. According to the standardized Cockroft-Gault formula, 14 of the identified CKD cases were hypertensive (46.7%); eight of them (57.1%) had a prior diagnosis of hypertension but none achieved therapeutic targets. Four of the identified CKD cases were diabetic (13.3%), three of whom had a prior diagnosis of diabetes mellitus with reasonably controlled blood sugar levels. Only age above 50 years and low educational level proved to be independent risk factors for CKD in multivariate analysis. Conclusion: The prevalence of CKD in the study population seems to be comparable to other countries. Keywords: awareness, chronic kidney disease, diabetes mellitus, hypertension, prevalence, Suda

Genetic variability, heritability and character association of grain yield and its components among selected genotypes of maize (Zea mays L.), Gezira State, Sudan

In the Sudan, the low yield of maize was mainly due to the use of low yielding land races and open-pollinated varieties. Hence, the current study was conducted to measure the extent of genotypic and phenotypic variability, genotypic performance, heritability, genotype x season interaction, and the magnitude of association among seed yield and its components of 10 maize genotypes. The experiment was carried out during the summer of 2017 and the winter of 2017/18, using a randomized complete block  design with three replicates, at the University of Gezira Farm, Wad Medani, Sudan. The characters measured were days to 50% tasseling and silking, plant and ear height, number of kernels per row and per ear, ear diameter, cob length, 100-kernels weight and grain yield. High significant phenotypic variability was detected for all the measured characters. Seasonal differences were also significant. High broad sense heritability estimates were recorded for 50% tasseling and silking and plant height while the rest of the characters showed moderate estimates. The study confirmed the fact that maize is a summer crop in central Sudan. In summer, the highest seed yield genotype was HSD 5158 (2609 kg/ha) and in winter was HSD 3538 (2285 kg/ha). Days to 50% tasseling and silking and plant height were significantly and positively correlated with yield and so recommended as selection criteria for seed yield improvement. It is recommended to grow genotypes HSD 5158 in summer and HSD 3538 in winter as well as genotypes VMH 4040, VMH 4102 and HSD 5007 in both seasons. Testing of these genotypes under different locations and seasons is suggested.              يعزى ضعف إنتاجية الذرة الشامية بالسودان لزراعة أصناف مفتوحة التلقيح وسلالات محلية ضعيفة الإنتاج. هدفت هذه الدراسة لقياس التباين الظاهري والوراثي ودرجة التوريث على المدى العريض وتفاعل الطراز الوراثي والموسم وارتباط إنتاج البذور ومكوناته. نفذت التجربة في صيف 2017 وشتاء 18/2017 بتصميم القطاعات العشوائية الكاملة بثلاث مكررات بمزرعة جامعة الجزيرة، واد مدني، السودان. الصفات التي  قيست هي : عدد الأيام لظهور 50% من الأزهار المذكرة والمؤنثة وطول النبات وارتفاع القندول وعدد بذور الصف والقندول ومحيط القندول وطول الكوز ووزن مائة حبة وإنتاج البذور. أظهرت النتائج اختلافاً معنوياً بين الطرز الوراثية لكل الصفات وفي الموسمين. تأثير الموسم كان معنوياً. سجلت قيم عالية لدرجة التوريث بالمعنى العريض لصفات 50% إزهار مذكر ومؤنث وطول النبات ولكن الصفات الأخرى أظهرت قيم وسطية. ثبت أن محصول الذرة الشامية في وسط السودان محصول صيفي. الطراز الذي أعطى أعلى إنتاجية صيفاً هو HSD 5158 (2609 كجم/هـ) وشتاءً  هو  HSD 3538 (2285 كجم/هـ). ارتبطت صفات الإزهار المؤنث والمذكر وطول النبات ارتباطاً موجباً ومعنوياً بالإنتاجية وعليه يمكن استعمالها كمؤشرات انتخاب لتحسين إنتاجية الحبوب. يوصى بزراعة الطراز HSD 5158  صيفاً والطراز HSD 3538 شتاءً والطرز VMH 4040 و VMH 4102 و HSD 5007 في الموسمين معاً. يوصى بزراعة هذه الطرز المقترحة في مواسم ومواقع متعددة لتأكيد النتائج. &nbsp