Changes in the Protein Profile of Streptomyces griseus during a Cycloheximide Fermentation

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/75202/1/j.1749-6632.1987.tb23846.x.pd

Requirement of Dynactin p150Glued Subunit for the Functional Integrity of the Keratinocyte Microparasol

The keratinocyte microparasol, composed of a perinuclear microtubular/melano–phagolysosomal complex, protects the nucleus from UV-induced DNA damage. We have previously demonstrated that cytoplasmic dynein is the motor involved in the perinuclear-directed aggregation of phagocytosed melanosomes. Dynactin, of which p150Glued is the major subunit, can link directly to microtubules and links organelles to dynein at different domains. To further define the mechanism of the microparasol, we transfected siRNA targeted against p150Glued into human keratinocytes cultured with 0.5mm fluorescent microspheres and performed time-lapse analysis, confocal immunolocalization, and Western immunoblotting after 24 and 48 hours. Western blots revealed a significant knockdown of the p150Glued subunit. The knockdown decreased p150Glued colocalization with microtubules and decreased perinuclear positioning of the convergent microtubular framework. It also inhibited perinuclear aggregation of phagocytosed fluorescent microspheres and reduced mean centripetal microsphere displacement. The findings provide evidence that dynactin p150Glued plays an important role in the functional integrity of the keratinocyte microparasol

Student Perceptions Of Appropriate Classroom Policies Of College Professors

Student perceptions of classroom management practices and policies employed by college educators have not been widely studied.  Faculty have broad discretion to determine classroom management practices and policies, and faculty are generally evaluated at least annually with student evaluations of teaching as a significant component of  the evaluative process.  The focus of this paper is whether students perceive faculty as having the freedom or discretion to adopt specified behaviors or policies unrelated to course content decisions.&nbsp

A Convenient Method for the Laboratoy Preparation of Absolute Ethyl Alcohol

The rates of dehydration of ethyl alcohol of 95 per cent strength by calcium oxide were determined at 15°, 45°, and 78°. By two successive treatments for two months each with lime at room temperature, the alcohol was found to be dehydrated in a satisfactory manner. The same result was found to be accomplished at 45° by two treatments of one week each. In neither case is refluxing necessary

A tail withdrawal procedure for assessing analgesic activity in rhesus monkeys

Rhesus monkeys were restrained in chairs from which their tails hung free so that their tails could be immersed into a thermos of water. Monkeys consistently kept their tails in 38-40[deg]C water for at least 20 sec, but withdrew them from 55[deg]C water in 1-4 sec. Tail withdrawal latencies from 55[deg]C water remained consistent over a period of 3 hr. Morphine produced dose-dependent increases in tail withdrawal latencies from 55[deg]C water, whereas pentobarbital, haloperidol, and phencyclidine did not increase tail withdrawal latencies except at doses that produced marked sedation.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/26151/1/0000228.pd

Computer modeling of antibiotic fermentation with on-line product removal

The fermentation of Streptomyces griseus for the production of cycloheximide is similar to other antibiotic fermentations in that product synthesis is subject to feedback regulation and the desired product is degraded in the fermentation broth. The productivity of this fermentation can thus be dramatically increased by removing the antibiotic from the whole broth as it is produced. One means for effecting this on-line product removal is to contact the whole fermentation broth with neutral polymeric resin immobilized in hydrogel beads. The antibiotic adsorbs to the immobilized resin via hydrophobic interactions. In this work, the adsorption of the antibiotic onto the immobilized resin was characterized. A biochemical model of the fermentation was then used to describe the time profiles of biomass, substrate, and antibiotic in a fermentation system in which whole broth is circulated from the fermentor through a continuously stirred extractor containing the adsorbent beads. Various operating conditions were examined to optimize the productivity of the fermentation.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/37896/1/260320314_ftp.pd