Fermentation and oxygen transfer in microgravity

The need for high rate oxygen transfer in microgravity for a Controlled Ecological Life Support System (CELSS) environment offers a number of difficulties and challenges. The use of a phase separated bioreactor appears to provide a way of overcoming these problems resulting in a system capable of providing high cell densities with rapid fermentation rates. Some of the key design elements are discussed

Gravitational Lensing by Dark Matter Halos with Non-universal Density Profiles

The statistics of gravitational lensing can provide us with a very powerful probe of the mass distribution of matter in the universe. By comparing predicted strong lensing probabilities with observations, we can test the mass distribution of dark matter halos, in particular, the inner density slope. In this letter, unlike previous work that directly models the density profiles of dark matter halos semi-analytically, we generalize the density profiles of dark matter halos from high-resolution N-body simulations by means of generalized Navarro-Frenk-White (GNFW) models of three populations with slopes, $\alpha$, of about -1.5, -1.3 and -1.1 for galaxies, groups and clusters, respectively. This approach is an alternative and independent way to examine the slopes of mass density profiles of halos. We present calculations of lensing probabilities using these GNFW profiles for three populations in various spatially flat cosmological models with a cosmological constant $\Lambda$. It is shown that the compound model of density profiles does not match well with the observed lensing probabilities derived from the Jodrell-Bank VLA Astrometric Survey data in combination with the Cosmic Lens All-Sky Survey data. Together with the previous work on lensing probability, our results suggest that a singular isothermal sphere mass model of less than about 10^{13}h^{-1}M_{\sun} can predict strong lensing probabilities that are consistent with observations of small splitting angles.Comment: 11 pages, 2 figures, Accepted by ApJL for publication (February 10 issue 2004

A Combined EIS-NVSS Survey Of Radio Sources (CENSORS) III: Spectroscopic observations

The Combined EIS-NVSS Survey Of Radio Sources (CENSORS) is a 1.4GHz radio survey selected from the NRAO VLA Sky Survey (NVSS) and complete to a flux-density of 7.2mJy. It targets the ESO Imaging Survey (EIS) Patch D, which is a 3 by 2 square degree field centred on 09 51 36.0, -21 00 00 (J2000). This paper presents the results of spectroscopic observations of 143 of the 150 CENSORS sources. The primary motivation for these observations is to achieve sufficient spectroscopic completeness so that the sample may be used to investigate the evolution of radio sources. The observations result in secure spectroscopic redshifts for 63% of the sample and likely redshifts (based on a single emission line, for example) for a further 8%. Following the identification of the quasars and star-forming galaxies in the CENSORS sample, estimated redshifts are calculated for the remainder of the sample via the K-z relation for radio galaxies. Comparison of the redshift distribution of the CENSORS radio sources to distributions predicted by the various radio luminosity function evolution models of Dunlop & Peacock 1990, results in no good match. This demonstrates that this sample can be used to expand upon previous work in that field.Comment: Accepted for publication in MNRAS. This version has some reduced resolution figures and 13 associated gif files. A version with all figures incorporated (at full resolution) is available at http://www.roe.ac.uk/~pnb/papers/censors_spectro.pd

Optical off-nuclear spectra of quasar hosts and radio galaxies

We present optical (~3200A to ~9000A) off-nuclear spectra of 26 powerful active galaxies in the redshift range 0.1 < z < 0.3, obtained with the Mayall and William Herschel 4-meter class telescopes. The sample consists of radio-quiet quasars, radio-loud quasars (all with -23 > M_V > -26) and radio galaxies of Fanaroff & Riley Type II (with extended radio luminosities and spectral indices comparable to those of the radio-loud quasars). The spectra were all taken approximately 5 arcseconds off-nucleus, with offsets carefully selected so as to maximise the amount of galaxy light falling into the slit, whilst simultaneously minimising the amount of scattered nuclear light. The majority of the resulting spectra appear to be dominated by the integrated stellar continuum of the underlying galaxies rather than by light from the non-stellar processes occurring in the active nuclei, and in many cases a 4000A break feature can be identified. The individual spectra are described in detail, and the importance of the various spectral components is discussed. Stellar population synthesis modelling of the spectra will follow in a subsequent paper (Nolan et al. 2000).Comment: 23 pages, LaTeX, uses MNRAS style file, incorporates 71 postscript figures, to be published in MNRAS. Contact author: [email protected]

Nanotrapping and the thermodynamics of optical tweezers

Particles that can be trapped in optical tweezers range from tens of microns down to tens of nanometres in size. Interestingly, this size range includes large macromolecules. We show experimentally, in agreement with theoretical expectations, that optical tweezers can be used to manipulate single molecules of polyethylene oxide suspended in water. The trapped molecules accumulate without aggregating, so this provides optical control of the concentration of macromolecules in solution. Apart from possible applications such as the micromanipulation of nanoparticles, nanoassembly, microchemistry, and the study of biological macromolecules, our results also provide insight into the thermodynamics of optical tweezers.Comment: 5 pages, 3 figures, presented at 17th AIP Congress, Brisbane, 200

Orientation of biological cells using plane-polarized Gaussian beam optical tweezers

Optical tweezers are widely used for the manipulation of cells and their internal structures. However, the degree of manipulation possible is limited by poor control over the orientation of trapped cells. We show that it is possible to controllably align or rotate disc shaped cells - chloroplasts of Spinacia oleracea - in a plane polarised Gaussian beam trap, using optical torques resulting predominantly from circular polarisation induced in the transmitted beam by the non-spherical shape of the cells.Comment: 9 pages, 6 figure

Model system studies with a phase separated membrane bioreactor

The operation and evaluation of a bioreactor designed for high intensity oxygen transfer in a microgravity environment is described. The reactor itself consists of a zero headspace liquid phase separated from the air supply by a long length of silicone rubber tubing through which the oxygen diffuses in and the carbon dioxide diffuses out. Mass transfer studies show that the oxygen is film diffusion controlled both externally and internally to the tubing and not by diffusion across the tube walls. Methods of upgrading the design to eliminate these resistances are proposed. Cell growth was obtained in the fermenter using Saccharomyces cerevisiae showing that this concept is capable of sustaining cell growth in the terrestial simulation

The final two redshifts for radio sources from the equatorial BRL sample

Best, Rottgering and Lehnert (1999, 2000a) defined a new sample of powerful radio sources from the Molonglo Reference Catalogue, for which redshifts were compiled or measured for 177 of the 178 objects. For the final object, MRC1059-010 (3C249), the host galaxy is here identified using near-infrared imaging, and the redshift is determined from VLT spectroscopy. For one other object in the sample, MRC0320+053 (4C05.14), the literature redshift has been questioned: new spectroscopic observations of this object are presented, deriving a corrected redshift. With these two results, the spectroscopic completeness of this sample is now 100%. New redshifts are also presented for PKS0742+10 from the Wall & Peacock 2.7 GHz catalogue, and PKS1336+003 from the Parkes Selected Regions. PKS0742+10 shows a strong neutral hydrogen absorption feature in its Lyman-alpha emission profile.Comment: 4 pages. LaTeX. Accepted for publication in MNRA