Genotoxicity of 2-alkylcyclobutanones, markers for an irradiation treatment in fat-containing food - Part I: cyto- and genotoxic potential of 2-tetradecylcyclobutanone

Previous experiments had indicated a slight genotoxic potential both in rat and in human colon cells of a sample of 2-dodecylcyclobutanone, a compound formed by irradiation of food containing palmitic acid in its triglycerides. Up to date, there is no evidence that 2-alkylcyclobutanones occur in non-irradiated foodstuffs, consequently it is prudent to test several members of the class of 2-alkylcyclobutanones which are produced by treatment of fat-containing food with ionising radiation. In this work, 2-tetradecylcyclobutanone (derived from stearic acid) has been tested for its cytotoxic and genotoxic potential. Human colon tumor cell lines, i.e. HT 29 and HT 29 clone 19A, were employed as models for in vitro experiments for cytotoxicity and genotoxicity tests. Cytotoxicity was measured by tetrazolium salt reduction assays (MTT and WST-1) and genotoxicity by determining DNA damage using the Comet Assay. Neither cytotoxic nor genotoxic effects were induced by 2-TCB in HT 29 or HT 29 cl 19A cells at an incubation time of 30 min at 37degreesC, not even at the highest concentration (400 muM) tested. After prolonged incubation times (1-2 days) at higher concentrations (>50 muM) cytotoxicity did, however, appear. Studies on other 2-alkylcyclobutanones are in progress. (C) 2002 Elsevier Science Ltd. All rights reserved

Lebensmittelbestrahlung

The conference was a combined event and at the same time was a meeting of the FAIR programme of the EU, under the responsibility of the General Directorate XII, participating countries including Iceland, Norway, Hungary, and Switzerland in addition to the 15 EU member states. Under this roof, research work is sponsored in the fields of food technology, fishing industry, agriculture, forestry, and water resources management. Also, financial support is available for the mid-range food and agricultural industry, or for projects promoting rural development. There currently are over 120 transnational FAIR projects, involving more than 2000 researchers in 233 EU-sponsored research projects devoted to food aspects, some having been presented at the conference. (orig./CB)Die Tagung ist zugleich auch eine Veranstaltung des FAIR-Programmes der EU, das von der Generaldirektion XII betreut wird. Neben den 15 EU-Mitgliedstaaten sind noch Island, Norwegen, Ungarn und die Schweiz beteiligt. Forschungsarbeiten in den Bereichen Lebensmitteltechnologie, Fischerei, Land-, Forst- und Wasserwirtschaft werden ueber Kooperationsvorhaben gefoerdert; daneben wird auch direkt die mittelstaendische Lebensmittel- und Agrarindustrie gefoerdert; daneben wird auch direkt die mittelstaendische Lebensmittel- und Agrarindustrie gefoerdert sowie Projekte der laendlichen Entwicklung finanziert werden. Die FAIR-Projekte bestehen zur Zeit aus ueber 120 transnationalen Vorhaben, mehr als 2000 Forscher sind Teilnehmer an insgesamt 233 EU-Forschungsvorhaben im Lebensmittelbereich; darunter auch Teilnehmer der Tagung. (orig.SR)SIGLEAvailable from TIB Hannover: RN 2898(99-01) / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekDEGerman

Bibliographie zur Bestrahlung von Lebensmitteln

TIB: RN 2898(34) / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekSIGLEDEGerman

Bibliographie zur Bestrahlung von Lebensmitteln

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Bibliographie zur Bestrahlung von Lebensmitteln. Nr. 30

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Ein erster Ringversuch zur Identifizierung strahlenbehandelter Gewuerze mit Hilfe von Lumineszenzmessungen

SIGLECopy held by FIZ Karlsruhe; available from UB/TIB Hannover / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekDEGerman

Bibliographie zur Bestrahlung von Lebensmitteln. No. 29

SIGLEAvailable from Bundesforschungsanstalt fuer Ernaehrung, Karlsruhe (DE) / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekDEGerman