The lysosomal enzyme activities of fresh, cooled, frozen and smoked salmon fish species (Onchorhyncus keta and Salmo salar)

WOS: 000087199100001Frozen-thawed fish and fillets are being marketed as fresh fish fillets. One of the methods which helps us to differentiate unfrozen from frozen-thawed fish Fillet is the enzymatic method. By means of the freezing and thawing process lysosomal enzymes are released, Lysosomal enzyme activities have the enzymes which decompose protein, carbohydrate, fat and nucleic acids. In this research, fresh, stored in ice, frozen and smoked salmon fish (Onchorhyncus keta and Saimo salar) have been used. In press juice and in extracts of these fish a muscle lysosomal enzyme alpha-Glucosidase (E.C. 3.2.1.20) in activitation quantities was detected. The lysosomal enzyme activities of the press juice of frozen-thawed salmon fish fillets were observed to be higher than those of fresh salmon fish fillets. The alpha-Glucosidase activity (Delta E(450)x10(-2)h(-1) x mg protein) was detected as 0.151 (at the beginning) in fresh fillets of salmon fish and that value of the salmon fish which are stored in ice was observed to have increased to 0.309 by the 9th day. The activity in frozen-thawed fillets was detected as 1.185 to 1.503. The alpha-Glucosidase activity of smoked salmon with different storage expiry periods was observed to be 0.969-4.51. At the end of the research it can be said that the lisosomal enzyme alpha-Glucosidase (E.C. 3.2.1.20) can be used to differentiate between processed, nonprocessed, fresh and frozen-thawed fish and fillets

Seawater gel in allergic rhinitis: entrapment effect and mucociliary clearance compared with saline

Objective: We performed a prospective study to investigate the the efficacy of seawater gel in reducing symptoms in patients with mild allergic rhinitis. We also aimed to investigate the impact of nasal irrigation on mucociliary clearance with seawater gel compared with saline in this patient group. Methods: The study was performed in 100 consecutive adult individuals with a history of allergic rhinitis that was not controlled by anti-allergic drugs. Patients were assigned to receive seawater gel nasal spray for 10 days. The efficacy of treatment was assessed by means of total nasal symptom score and clinical findings. Results: A statistically significant difference was found between scores of ‘nasal discharge, nasal obstruction, sneezing, nasal itching’before and after treatment ( p < 0.001). Clinical findings evaluation revealed a statistically significant decrease in lower turbinate colour rating and turbinate congestion at the end of treatment ( p < 0.001). Saccharin transit time decreased from baseline in the seawater trials by 12% compared with a 4% decrease for saline. The difference between the percent changes was statistically significant ( t = 2.177; p < 0.05). Conclusions: The present study provides evidence that a four times daily regimen of seawater gel can be an adjunctive therapy in the patient with allergic rhinitis