Design and evaluation of a unique RT-qPCR assay for diagnostic quality control assessment that is applicable to pathogen detection in three species of salmonid fish

BACKGROUND: The detection of pathogens at early stages of infection is a key point for disease control in aquaculture. Therefore, accurate diagnostic procedures are a must. Real-time PCR has been a mainstay in diagnostics over the years due to its speed, specificity, sensitivity, reproducibility and throughput; as such, real-time PCR is a target for improvement. Nevertheless, to validate a novel diagnostic tool, correct setup of the assay, including proper endogenous controls to evaluate the quantity and quality of the samples and to detect possible sample degradation, is compulsory. This work aims to design a unique RT-qPCR assay for pathogen detection in the three salmonid species reared in Chile. The assay uses elongation factor 1 alpha as the single endogenous control, thus avoiding the need for multiple endogenous controls, as well as multiple validations and non-comparable quality control parameters. RESULTS: The in vivo and in vitro analyses of samples from Salmo salar, Oncorhynchus mykiss and Oncorhynchus kisutch showed that when primers were accurately selected to target conserved regions of the elongation factor 1 alpha (ELF1α) gene, a single novel RT-qPCR assay yielding similar and reproducible Ct values between the three species could be designed. The opposite occurred when an assay originally designed for Salmo salar was tested in samples from the two species of the genus Oncorhynchus. CONCLUSIONS: Here, we report the design and evaluation of an accurate trans-species RT-qPCR assay that uses the elongation factor 1 alpha (ELF1α) gene as an endogenous control and is applicable for diagnostic purposes in samples obtained from the three salmonid species reared in Chile

1005-81 Myocardial Protection by Na+/H+ Exchange Inhibition in Ischemic, Reperfused Porcine Hearts

The protective effect of the Na+/H+ exchange inhibitor HOE 694 was tested in porcine hearts subjected to 45min of regional ischemia and 24 h of reperfusion. The compound (3mg/kg) was intravenously injected in 6 pigs each either 10 min before ischemia (group A) or 10 min before reperfusion (group B). Six animals served as controls. Apart from the main end-points, infarct size and regional systolic shortening, the effect of HOE 694 on global hemodynamic parameters which included coronary blood flow and coronary venous oxygen saturation was evaluated. Although the Na+IH+ exchange inhibitor did not affect global hemodynamics, preischemic treatment with HOE 694 decreased infarct size from 65±18% (control group) to 12 ± 9% (p < 0.01) and improved systolic shortening from 8 ± 6% (control group) to 28 ± 9% P < 0.02). In addition, increase in heart rate and myocardial contracture during early reperfusion were significantly attenuated in group A. Treatment of group B did not exhibit protective effects.ConclusionNa+/H+ exchange inhibition is a very protective means in myocardial ischemia and reperfusion when administered before ischemia. In this model, it was ineffective when given before reperfusion

Competing electric and magnetic excitations in backward electron scattering from heavy deformed nuclei

Important $E2$ contributions to the $(e,e^{\prime})$ cross sections of low-lying orbital $M1$ excitations are found in heavy deformed nuclei, arising from the small energy separation between the two excitations with $I^{\pi}K = 2^+1$ and 1$^+1$, respectively. They are studied microscopically in QRPA using DWBA. The accompanying $E2$ response is negligible at small momentum transfer $q$ but contributes substantially to the cross sections measured at $\theta = 165 ^{\circ}$ for $0.6 < q_{\rm eff} < 0.9$ fm$^{-1}$ ($40 \le E_i \le 70$ MeV) and leads to a very good agreement with experiment. The electric response is of longitudinal $C2$ type for $\theta \le 175 ^{\circ}$ but becomes almost purely transverse $E2$ for larger backward angles. The transverse $E2$ response remains comparable with the $M1$ response for $q_{\rm eff} > 1.2$ fm$^{-1}$ ($E_i > 100$ MeV) and even dominant for $E_i > 200$ MeV. This happens even at large backward angles $\theta > 175 ^{\circ}$, where the $M1$ dominance is limited to the lower $q$ region.Comment: RevTeX, 19 pages, 8 figures included Accepted for publication in Phys Rev

Collective Excitations of (154)Sm nucleus at FEL{gamma}+LHC Collider

The production of collective excitations of the (154)Sm at FEL{gamma}+LHC collider is investigated. We show that this machine will be a powerful tool for investigation of high energy level excitations.Comment: 6 pages, 1 figure, 4 table

Large-Scale Transition of Economic Systems Do CEECs Converge Towards Western Prototypes?

In order to identify convergence patterns among the group of Central and Eastern European Countries (CEECs) we analyze clusters of traditional OECD countries, i.e. EU-15 plus Norway and Switzerland, Anglo-Saxon non-EU countries plus Japan, and CEECs based on macro data on government regulation and spending instead of micro data on firm relations and market characteristics as is usually applied in Varieties-of-Capitalism (VoC) analysis. This framework is supposed to incorporate some of the critique that has been expressed towards the traditional VoCapproach, especially its ignorance of government spending and performance. We acknowledge for the transition aspect by looking at cluster history and principal component analysis for periods of transition. Our analysis reveals that there is consolidation rather than convergence with CEECs being divided in clusters leaning towards CME and LME prototypes respectively. Overall, there are worlds of redistribution within which clusters differ with respect to their mix of - negatively correlated - regulation and innovation. Interestingly, CEECs do not mix up with Mediterranean MMEs, which indeed provide a kind of worst case setting, while Scandinavian CMEs as well as traditional LMEs provide a kind of role model within their respective worlds of redistribution

Investment case for two-year post university speciality training in family medicine in Tajikistan: how much is needed for continuing and scaling up the improved education of family doctors?

Background: A new two-year Post University Specialty Training (PUST) programme in family medicine was introduced to improve the quality of postgraduate speciality medical education in Tajikistan. Postgraduate education of family doctors (FDs) needs to be urgently scaled up, as 38% of FD positions in Tajikistan remained unfilled in 2018. Moreover, the international financial support for the PUST programme is ending. This investment case assesses the minimum funding needed for the continuation and scale-up of PUST and establishes the rationale for the investment in the light of a recent evaluation. Methods: The costs of the programme were calculated for 2018 and a scale-up forecast made for the period 2019-2023. The impact of the scale-up on the shortage of FDs was assessed. An evaluation using a Multiple Choice Questionnaire and Objective Structured Clinical Examination (OSCE) assessed and compared theoretical knowledge, clinical skills and competencies of PUST trained and conventionally trained FDs. Results: The annual costs of the programme were US$228,000 in 2018. The total investment needed for scaling up PUST from 31 new FDs in 2018 to 100 FD graduates each year by 2023 was US$ 802,000.However, when the retirement of FDs and population growth are considered, the scale-up will result only in maintaining the current level of FDs working and not solve the country's FD shortage. The PUST FDs demonstrated significantly better clinical skills than the conventionally trained interns, scoring 60 and 45% of OSCE points, respectively. Theoretical knowledge showed a similar trend; PUST FDs answered 44% and interns 38% of the questions correctly. Conclusions: The two-year PUST programme has clearly demonstrated it produces better skilled family doctors than the conventional one-year internship, albeit some enduring quality concerns do still prevail. The discontinuation of international support for PUST would be a major setback and risks potentially losing the benefits of the programme for family medicine and also other specialities. To guarantee the supply of adequately trained FDs and address the FD shortage, the PUST should be continued and scaled up. Therefore, it is essential that international support is extended and a gradual transition to sustainable national financing gets underway

Medical education reform in Tajikistan: comparison of the conventional one-year family medicine residency program and the new two-year residency program for postgraduate medical education

INTRODUCTION: The last two decades have seen a shift in former Soviet countries from highly specialized to more family medicine-focused systems. Medical education has slowly adjusted to these reforms, although the region is still at risk to have a chronic shortage of family doctors. This paper presents the evaluation of a new post-graduate family medicine program in Tajikistan, focused on competency-based training. The findings are relevant for policy makers, international organizations and practitioners participating in similar medical education reform programs. METHODS: We employed a quasi-experimental control group design and compared intervention residents, control group residents with traditional training, and 1st year residents with no training in two outcomes, clinical knowledge and competencies. We employed two objective measures, a written multiple-choice question test (MCQT) and an Objective Structured Clinical Examination (OSCE), respectively. We report reliability and validity of the measures along with ANOVA, planned contrasts and effect size estimates to examine differences across groups. RESULTS: We found statistically significant differences in both clinical knowledge and competencies between intervention and control groups. We also detected a large intervention effect size. Participants in the intervention outperformed control group participants in the two measures. Our analysis suggests that intervention and control group participants are comparable in terms of initial knowledge and competencies, strengthening the argument that the intervention caused the improvement in the program outcomes. DISCUSSION: Receiving tailored training and structured opportunities to practice knowledge and competencies in clinical settings have a positive effect on the education of family medicine doctors in Tajikistan. Our results support curriculum reform and investment in medical education in the form of longer and supervised on-the-job preparation designed to be more in line with international standards. We discuss suggestions for future studies and potential requirements to inform replicability in other countries. CONCLUSION: Family medicine is well recognized as central to health systems throughout the world, but high quality residency training lags behind in some countries. Our study showed that investing in family medicine residency programs and structured training is effective in increasing critical clinical competencies. We encourage promoting comprehensive post graduate family medicine doctor training so that the goals of a family medicine centered health system are attainable

F-spin as a Partial Symmetry

We use the empirical evidence that F-spin multiplets exist in nuclei for only selected states as an indication that F-spin can be regarded as a partial symmetry. We show that there is a class of non-F-scalar IBM-2 Hamiltonians with partial F-spin symmetry, which reproduce the known systematics of collective bands in nuclei. These Hamiltonians predict that the scissors states have good F-spin and form F-spin multiplets, which is supported by the existing data.Comment: 14 pages, 1 figur