5 research outputs found
Antibiotic Uptake into Bacteria: Negamycin Translocation Across the Outer and Cytoplasmic Membranes of Escherichia coli
Die Dissertation ist gesperrt bis zum 27. Februar 2025 !This thesis is under embargo until 27 February 2025
Kanamycin Uptake into Escherichia coli Is Facilitated by OmpF and OmpC Porin Channels Located in the Outer Membrane
Kanamycin Uptake into Escherichia coli Is Facilitated by OmpF and OmpC Porin Channels Located in the Outer Membrane
Despite decades of therapeutic application of aminoglycosides, it is still a matter of debate if porins contribute to the translocation of the antibiotics across the bacterial outer membrane. Here, we quantified the uptake of kanamycin across the major porin channels OmpF and OmpC present in the outer membrane of Escherichia coli. Our analysis revealed that, despite its relatively large size, about 10-20 kanamycin molecules per second permeate through OmpF and OmpC under a 10 ÎĽM concentration gradient, whereas OmpN does not allow the passage. Molecular simulations elucidate the uptake mechanism of kanamycin through these porins. Whole-cell studies with a defined set of E. coli porin mutants provide evidence that translocation of kanamycin via porins is relevant for antibiotic potency. The values are discussed with respect to other antibiotics
Kanamycin Uptake into E. Coli Is Facilitated by OmpF and OmpC Porin Channels Located in the Outer Membrane
Despite decades of
therapeutic application of aminoglycosides, it is still a matter of debate if porins
contribute to the translocation of the antibiotics across the bacterial outer
membrane. Here, we quantified the uptake of kanamycin across the major porin channels
OmpF and OmpC present in the outer membrane of E. coli. Our analysis revealed that, despite its relatively large
size, about 10 - 20 kanamycin molecules per second permeate
through OmpF and OmpC under a 10 mM concentration gradient, whereas OmpN does not allow
the passage. Molecular simulations elucidate the uptake mechanism of kanamycin
through these porins. Whole-cell
studies with a decisive set of E. coli
porin mutants provide evidence that translocation of kanamycin via porins is relevant for antibiotic
potency.</p