Souche bacterienne et melange bacterien a activite fucanolytique

The present invention relates to a bacterial strain producing enzymes with fucanolytic activity, a bacterial mixture comprising said strain, the use of said strain or a bacterial mixture comprising it for the production of enzymes with fucanolytic activity, the enzymes obtained by culturing said strain or bacterial mixture comprising it, a process for depolymerizing fucan by using said strain or a bacterial mixture comprising it or the enzymes obtained and the fucan fragments obtained by this method.La présente invention concerne une souche bactérienne productrice d'enzymes à activité fucanolytique, un mélange bactérien comprenant ladite souche, l'utilisation de ladite souche ou du mélange bactérien la comprenant pour la production d'enzymes à activité fucanolytique, les enzymes obtenues par la culture de ladite souche, un procédé de dépolymérisation du fucane en mettant en œuvre ladite souche ou un mélange bactérien la comprenant ou lesdites enzymes et les fragments de fucane obtenus par ce procédé

Discrimination of neutral oligosaccharides through a nanopore

International audienceThe detection of oligosaccharides at the single-molecule level was investigated using a protein nanopore device. Neutral oligosaccharides of various molecular weights were translocated through a single α-hemolysin nanopore and their nano-transit recorded at the single-molecule level. The translocation of maltose and dextran oligosaccharides featured by 1 → 4 and 1 → 6 glycosidic bonds respectively was studied in an attempt to discriminate oligosaccharides according to their polymerization degree and glycosidic linkages. Oligosaccharides were translocated through a free diffusion regime indicating that they adopted an extended conformation during their translocation in the nanopore. The dwell time increased with molecular mass, suggesting the usefulness of nanopore as a molecular sizing device