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Not AvailableThis book covered the current knowledge on structural as well as functional attributes of Heat shock protein 70 (Hsp70), which is the prime as well as abundant stressor protein in biological systems. In this book, the authors discuss research including the Heat Shock Proteins: History, Discoveries, and Implications in Diseases, Therapeutic and Research Options; Intracellular Versus Extracellular Hsp70; Role of Hsp70 in Sperm Quality; Heat Stress and Livestock Adaptation: An HSP70 Perspectives; Bovine Heat Shock Protein 70 (Hsp70) and Its Role In Thermoregulation; The Role of Hsp70 in Sperm Quality; comprehensive review on HSP70 in shrimps and role of microbial heat shock protein70 (mHSP70) in the development of veterinary vaccines. This book will be convenient for researchers engaged in the fields of Medicine, Physiology, Clinical Trials, Biotechnology, Molecular Medicine and Pathology.Not Availabl

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Not AvailableProlactin (PRL) and beta-lactoglobulin (BLG) are two important candidate genes well known to be associated with milk production traits as well as somatic cell count (SCC) among dairy cattle breeds. In the present study, the intron 3 of PRL and the spanning region between exon IV and intron IV of the BLG gene were chosen for genotyping and their association with milk production traits as well as SCC among HF crossbred cattle (i.e., Frieswal) that originated from India. We observed that the AA genotype frequency of PRL among Frieswal cows is higher than that of AB and BB. Our findings showed that cows with AA and BB genotypes had significantly (p < 0.05) higher total milk yield and peak yield than AB genotype cows. Comparing SCC with various genotypic groups, we observed that BB genotype cows had significantly (p < 0.05) lower SCC than those with AB and AA genotypes. In the case of BLG, the genotypic frequency of BB was higher than that of AB and AA. The AB and BB genotypes of BLG had a significant (p < 0.05) effect on total milk yield and peak yield compared with AA. The SCC of the AA genotype of BLG is significantly (p < 0.05) lower than that of AB and BB. This study thus indicates that AA and BB genotypes in PRL as well as AB and BB genotypes in BLG may be more suitable for better milk production; however, cows having BB genotype in PRL and AA genotype in BLG may show more resistance to mastitis than those with other genotypes among HF crossbred cattle.Not Availabl

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Not AvailableProlactin (PRL) and beta-lactoglobulin (BLG) are two important candidate genes well known to be associated with milk production traits as well as somatic cell count (SCC) among dairy cattle breeds. In the present study, the intron 3 of PRL and the spanning region between exon IV and intron IV of the BLG gene were chosen for genotyping and their association with milk production traits as well as SCC among HF crossbred cattle (i.e., Frieswal) that originated from India. We observed that the AA genotype frequency of PRL among Frieswal cows is higher than that of AB and BB. Our findings showed that cows with AA and BB genotypes had significantly (p < 0.05) higher total milk yield and peak yield than AB genotype cows. Comparing SCC with various genotypic groups, we observed that BB genotype cows had significantly (p < 0.05) lower SCC than those with AB and AA genotypes. In the case of BLG, the genotypic frequency of BB was higher than that of AB and AA. The AB and BB genotypes of BLG had a significant (p < 0.05) effect on total milk yield and peak yield compared with AA. The SCC of the AA genotype of BLG is significantly (p < 0.05) lower than that of AB and BB. This study thus indicates that AA and BB genotypes in PRL as well as AB and BB genotypes in BLG may be more suitable for better milk production; however, cows having BB genotype in PRL and AA genotype in BLG may show more resistance to mastitis than those with other genotypes among HF crossbred cattle

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Not AvailableThe milk is considered as a functional food used as a natural remedy in many part of world for the management of a number of human diseases for which it's purity is important. The Prevention of Food Adulteration Act (PFA) stipulates the supply of pure milk to the consumers. Presently, due to high market demand and less supply of camel milk, it is being adulterated with cow milk. As it is well known, food adulteration is a global concern and developing countries like India are at higher risk. So, developing robust, reliable and low cost molecular technique would be beneficial for monitoring the cow and cattle milk adulteration in labs provided with basic instrumentation facility. The CIRC-COWCAM assay kit is useful for detecting cow milk adulteration in camel milk and vice versa at a minimum level of 1%.Not Availabl

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Not AvailableITGB6 is known to be one of the major receptor components involved in host tropism of foot-andmouth disease (FMD) virus in cattle. A competitive PCR technique called ARMS PCR was adapted to identify a single-nucleotide polymorphism (SNP), G29A, db SNP Id: rs109075046, in the 50 untranslated region (50UTR) of the bovine ITGB6 gene. Genotype profiling identified three kinds of genetic variation within the targeted SNP among Frieswal crossbred cattle. The occurrence of FMD in the three genotypes was further evaluated, revealing a clear role in the incidence of FMD in the studied populatio

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Not AvailableThe freezing of bull semen significantly hamper the motility of sperm which reduces the conception rate in dairy cattle. The prediction of postthaw motility (PTM) before freezing will be useful to take the decision on discarding or freezing of the germplasm. The artificial neural network (ANN) methodology found to be useful in prediction and classification problems related to animal science, and hence, the present study was undertaken to compare the efficiency of ANN in prediction of PTM on the basis of the number of ejaculates, volume, and concentration of sperms. The combined effect of Y-specific microsatellite alleles on the actual and predicted PTM was also studied. The results revealed that the prediction accuracy of PTM based on the semen quality parameters was comparatively lower because of higher variability in the data set. The ANN gave better prediction accuracy (34.88%) than the multiple regression analysis models (32.04%). The root mean square error was lower for ANN (8.4353) than that in the multiple regression analysis (8.6168). The haplotype or combined effect of microsatellite alleles on actual and predicted PTM was found to be highly significant (P < 0.01). On the basis of results, it was concluded that the ANN methodology can be used for prediction of PTM in crossbred bulls.Not Availabl

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Not AvailableNa+/K+-ATPase is an integral membrane protein composed of a large catalytic subunit (alpha), a smaller glycoprotein subunit (beta), and gamma subunit. The beta subunit is essential for ion recognition as well as maintenance of the membrane integrity. Present study was aimed to analyze the expression pattern of ATPase beta subunit genes (ATPase B1, ATPase B2, and ATPase B3) among the crossbred bulls under different ambient temperatures (20–44 °C). The present study was also aimed to look into the relationship of HSP70 with the ATPase beta family genes. Our results demonstrated that among beta family genes, transcript abundance of ATPase B1 and ATPase B2 is significantly (P<0.05) higher during the thermal stress. Pearson correlation coefficient analysis revealed that the expression of ATPase Β1, ATPase B2, and ATPase B3 is highly correlated (P<0.01) with HSP70, representing that the change in the expression pattern of these genes is positive and synergistic. These may provide a foundation for understanding the mechanisms of ATPase beta family genes for cellular thermotolerance in cattleNot Availabl

MOESM1 of T-ARMS PCR genotyping of SNP rs445709131 using thermostable strand displacement polymerase

Additional file 1. Generation of Mutant genotypes/Genotyping of mutant using PCR–RFLP and T-ARMS PCR/Effect of use of 1 and 2 units of SD and Taq polymerase