3 research outputs found
Systematic evidence on migrating and extractable food contact chemicals: Most chemicals detected in food contact materials are not listed for use
Food packaging is important for todayâs globalized food system, but food contact materials (FCMs) can also be a source of hazardous chemicals migrating into foodstuffs. Assessing the impacts of FCMs on human health requires a comprehensive identification of the chemicals they contain, the food contact chemicals (FCCs). We systematically compiled the âdatabase on migrating and extractable food contact chemicalsâ (FCCmigex) using information from 1210 studies. We found that to date 2881 FCCs have been detected, in a total of six FCM groups (Plastics, Paper & Board, Metal, Multi-materials, Glass & Ceramic, and Other FCMs). 65% of these detected FCCs were previously not known to be used in FCMs. Conversely, of the more than 12â000 FCCs known to be used, only 1013 are included in the FCCmigex database. Plastic is the most studied FCM with 1975 FCCs detected. Our findings expand the universe of known FCCs to 14,153 chemicals. This knowledge contributes to developing non-hazardous FCMs that lead to safer food and support a circular economy
Integration of highârisk human papillomavirus into cellular cancerârelated genes in head and neck cancer cell lines
BackgroundHuman papillomavirus (HPV)âpositive oropharyngeal cancer is generally associated with excellent response to therapy, but some HPVâpositive tumors progress despite aggressive therapy. The purpose of this study was to evaluate viral oncogene expression and viral integration sites in HPV16â and HPV18âpositive squamous cell carcinoma lines.MethodsE6/E7 alternate transcripts were assessed by reverse transcriptaseâpolymerase chain reaction (RTâPCR). Detection of integrated papillomavirus sequences (DIPSâPCR) and sequencing identified viral insertion sites and affected host genes. Cellular gene expression was assessed across viral integration sites.ResultsAll HPVâpositive cell lines expressed alternate HPVE6/E7 splicing indicative of active viral oncogenesis. HPV integration occurred within cancerârelated genes TP63, DCC, JAK1, TERT, ATR, ETV6, PGR, PTPRN2, and TMEM237 in 8 head and neck squamous cell carcinoma (HNSCC) lines but UMâSCCâ105 and UMâGCCâ1 had only intergenic integration.ConclusionHPV integration into cancerârelated genes occurred in 7 of 9 HPVâpositive cell lines and of these 6 were from tumors that progressed. HPV integration into cancerârelated genes may be a secondary carcinogenic driver in HPVâdriven tumors. © 2017 Wiley Periodicals, Inc. Head Neck 39: 840â852, 2017Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/136719/1/hed24729_am.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/136719/2/hed24729.pd