Regulation of manganese superoxide dismutase in glomerular epithelial cells: Mechanisms for interleukin 1 induction

Regulation of manganese superoxide dismutase in glomerular epithelial cells: Mechanisms for interleukin 1 induction. Reactive oxygen species have been implicated as mediators of tissue injury in glomerular inflammation. The expression of the antioxidant enzyme, manganese superoxide dismutase (MnSOD), was examined in primary cultures of rat glomerularepithelial cells (GEC) in response to inflammatory mediators. The results demonstrate that GEC respond to interleukin-1 (IL-1) and bacterial lipopolysaccharride (LPS) with an increase in MnSOD steady-state mRNA levels. The IL-1α-mediated induction of MnSOD mRNA levels was both time- and dose-dependent. Maximal levels, approximately 40-fold above controls, were observed at 12 hours with 2 ng/ml of IL-1α. MnSOD protein levels were also markedly elevated by IL-1α. The induction of MnSOD mRNA by IL-1α required de novo transcription as well as some degree of protein synthesis. To elucidate the potential intracellular signal that mediates IL-1α-dependent MnSOD expression, three classical signaling pathways were examined. We found no evidence that MnSOD induction by IL-lα is mediated by either the cyclooxygenase or lipoxygenase pathway or via activation of protein kinase C. Based on the presence of IL-lα in several forms of glomerular inflammation, the observed increase in MnSOD expression by this immunoregulatory cytokine must have an important role in the antioxidant defense of glomerular epithelial cells

Highly charged ions in Penning traps, a new tool for resolving low lying isomeric states

The use of highly charged ions increases the precision and resolving power, in particular for short-lived species produced at on-line radio-isotope beam facilities, achievable with Penning trap mass spectrometers. This increase in resolving power provides a new and unique access to resolving low-lying long-lived ($T_{1/2} > 50$ ms) nuclear isomers. Recently, the $111.19(22)$ keV (determined from $\gamma$-ray spectroscopy) isomeric state in $^{78}$Rb has been resolved from the ground state, in a charge state of $q=8+$ with the TITAN Penning trap at the TRIUMF-ISAC facility. The excitation energy of the isomer was measured to be $108.7(6.4)$ keV above the ground state. The extracted masses for both the ground and isomeric states, and their difference, agree with the AME2003 and Nuclear Data Sheet values. This proof of principle measurement demonstrates the feasibility of using Penning trap mass spectrometers coupled to charge breeders to study nuclear isomers and opens a new route for isomer searches.Comment: 8 pages, 6 figure

Hadronic Parity Violation: a New View through the Looking Glass

Studies of the strangeness changing hadronic weak interaction have produced a number of puzzles that have so far evaded a complete explanation within the Standard Model. Their origin may lie either in dynamics peculiar to weak interactions involving strange quarks or in more general aspects of the interplay between strong and weak interactions. In principle, studies of the strangeness conserving hadronic weak interaction using parity violating hadronic and nuclear observables provide a complementary window on this question. However, progress in this direction has been hampered by the lack of a suitable theoretical framework for interpreting hadronic parity violation measurements in a model-independent way. Recent work involving effective field theory ideas has led to the formulation of such a framework while motivating the development of a number of new hadronic parity violation experiments in few-body systems. In this article, we review these recent developments and discuss the prospects and opportunities for further experimental and theoretical progress.Comment: Manuscript submitted to Annual Reviews of Nuclear and Particle Scienc

Extinction of the N=20 neutron-shell closure for 32Mg examined by direct mass measurements

The 'island of inversion' around $^{32}$Mg is one of the most important paradigm for studying the disappearance of the stabilizing 'magic' of a shell closure. We present the first Penning-trap mass measurements of the exotic nuclides $^{29-31}$Na and $^{30-34}$Mg, which allow a precise determination of the empirical shell gap for $^{32}$Mg. The new value of 1.10(3) MeV is the lowest observed shell gap for any nuclide with a canonical magic number.Comment: 6 pages, 4 figures, submitted to Physical Review

Enhanced dielectronic recombination of lithium-like Ti19+ ions in external ExB fields

Dielectronic recombination(DR) of lithium-like Ti19+(1s2 2s) ions via 2s->2p core excitations has been measured at the Heidelberg heavy ion storage ring TSR. We find that not only external electric fields (0 <= Ey <= 280 V/cm) but also crossed magnetic fields (30 mT <= Bz <= 80 mT) influence the DR via high-n (2p_j nl)-Rydberg resonances. This result confirms our previous finding for isoelectronic Cl14+ ions [Bartsch T et al, PRL 82, 3779 (1999)] that experimentally established the sensitivity of DR to ExB fields. In the present investigation the larger 2p_{1/2}-2p_{3/2} fine structure splitting of Ti19+ allowed us to study separately the influence of external fields via the two series of Rydberg DR resonances attached to the 2s -> 2p_{1/2} and 2s -> 2p_{3/2} excitations of the Li-like core, extracting initial slopes and saturation fields of the enhancement. We find that for Ey > 80 V/cm the field induced enhancement is about 1.8 times stronger for the 2p_{3/2} series than for the 2p_{1/2} series.Comment: 10 pages, 3 figures, to be published in Journal of Physics B, see also http://www.strz.uni-giessen.de/~k

Tensor interaction constraints from beta decay recoil spin asymmetry of trapped atoms

We have measured the angular distribution of recoiling daughter nuclei emitted from the Gamow-Teller $\beta$ decay of spin-polarized $^{80}$Rb. The asymmetry of this distribution vanishes to lowest order in the Standard Model (SM) in pure Gamow-Teller decays, producing an observable very sensitive to new interactions. We measure the non-SM contribution to the asymmetry to be $A_{T}$= 0.015 $\pm$ 0.029 (stat) $\pm$ 0.019 (syst), consistent with the SM prediction. We constrain higher-order SM corrections using the measured momentum dependence of the asymmetry, and their remaining uncertainty dominates the systematic error. Future progress in determining the weak magnetism term theoretically or experimentally would reduce the final errors. We describe the resulting constraints on fundamental 4-Fermi tensor interactions.Comment: 11 pages, 13 figures; v2 published in Phys. Rev. C, with referee clarifications and figures improved for black-and-whit