Glial reactions surrounding implants after one week.

<p>Example pictures of GFAP (magenta) & ED1 (green) staining from middle (A), outer (B), contralateral (C) & solitary (D) implant locations after one week. Scale bar 100 µm. Quantifications of GFAP & ED1 staining after one week in 0–50 µm ROI (E) & 50–200 µm ROI (F). Circles & error bars signify median values & interquartile ranges. X-axes show different implant locations. Y-axes show the fraction of area in each ROI that is above the set threshold. No statistically significant differences between the tissue reactions at the different implant locations was found in any of these groups, n = 6 for all groups.</p

Schematic overview of implant locations in the different study groups.

<p>Dorsal view of the cerebral cortex with implant locations indicated by black dots. The implant in the left hemisphere of Group 1 & 3 is referred to as the contralateral implant; the implants in the right hemisphere in group 1 & 3 are referred to as middle and outer implants respectively; the implant in the right hemisphere of group 2 & 4 is referred to as the solitary implant and has no contralateral counterpart. Distances between implants in the right hemisphere of group 1 & 3 are 1 mm. Unnamed implants are not analyzed.</p

Glial reactions surrounding implants after six weeks.

<p>Example pictures of GFAP (magenta) & ED1 (green) staining from middle (A), outer (B), contralateral (C) & solitary (D) implant locations after six weeks. Scale bar 100 µm. Quantifications of GFAP & ED1 staining after six weeks in 0–50 µm ROI (E) & 50–200 µm ROI (F). X-axes show different implant locations. Y-axes show the fraction of area in each ROI that is above the set threshold. The astrocytic scar surrounding the middle implant was significantly smaller than surrounding the contralateral implant in the inner ROI (p<0.01, K-W test with Dunn’s post hoc test). No difference was found for ED1 staining between any of the groups, n = 6 for all groups except “solitary” where n = 5.</p