The Global Impact of 1956: Race, Neutralism, and National Liberation

The University Archives has determined that this item is of continuing value to OSU's history.In 1956, the world witnessed two upheavals: a major rift in European communism, with the revolutions in Poland and Hungary; and the onset of decolonization after the abortive Suez invasion by France and Great Britain. In this 50th anniversary of that momentous year, we propose to look beyond these seminal events and explore their impact on three significant issues -– race, neutralism, and national liberation -– which continue to affect the world today.Web page announcement, conference program, conferene poster, conference photos, conference proceeding

Suppression of the PI3K subunit p85α delays embryoid body development and inhibits cell adhesion

Phosphatidylinositol-3-kinases (PI3Ks) exert a variety of signaling functions in eukaryotes. We suppressed the PI3K regulatory subunit p85α using a small interfering RNA (Pik3r1 siRNA) and examined the effects on embryoid body (EB) development in hanging drop culture. We observed a 150% increase in the volume of the treated EBs within 24 h, compared to the negative controls. Fluorescence Activated Cell Sorting (FACS) assays showed that this increase in volume is not due to increased cellular proliferation. Instead, the increase in volume appears to be due to reduced cellular aggregation and adherence. This is further shown by our observation that 40% of treated EBs form twin instead of single EBs, and that they have a significantly reduced ability to adhere to culture dishes when plated. A time course over the first 96 h reveals that the impaired adherence is transient and explained by an initial 12-hour delay in EB development. Quantitative PCR expression analysis suggests that the adhesion molecule integrin-β1 (ITGB1) is transiently downregulated by the p85α suppression. In conclusion we found that suppressing p85α leads to a delay in forming compact EBs, accompanied by a transient inability of the EBs to undergo normal cell-cell and cell-substrate adhesion.</p