Denoising Method Based on Sparse Representation for WFT Signal

Affected by external noise and various nature disturbances, Wheel Force Transducer (WFT) signal may be completely submerged, and the sensitivity and the reliability of measurement can be strongly decreased. In this paper, a new wavelet packet denoising method based on sparse representation is proposed to remove the noises from WFT signal. In this method, the problem of recovering the noiseless signal is converted into an optimization problem of recovering the sparsity of their wavelet package coefficients, and the wavelet package coefficients of the noiseless signals can be obtained by the augmented Lagrange optimization method. Then the denoised WFT signal can be reconstructed by wavelet packet reconstruction. The experiments on simulation signal and WFT signal show that the proposed denoising method based on sparse representation is more effective for denoising WFT signal than the soft and hard threshold denoising methods

Development of one-step SYBR Green real-time RT-PCR for quantifying bovine viral diarrhea virus type-1 and its comparison with conventional RT-PCR

<p>Abstract</p> <p>Background</p> <p>Bovine viral diarrhea virus (BVDV) is a worldwide pathogen in cattle and acts as a surrogate model for hepatitis C virus (HCV). One-step real-time fluorogenic quantitative reverse transcription polymerase chain reaction (RT-PCR) assay based on SYBR Green I dye has not been established for BVDV detection. This study aims to develop a quantitative one-step RT-PCR assay to detect BVDV type-1 in cell culture.</p> <p>Results</p> <p>One-step quantitative SYBR Green I RT-PCR was developed by amplifying cDNA template from viral RNA and using <it>in vitro </it>transcribed BVDV RNA to establish a standard curve. The assay had a detection limit as low as 100 copies/ml of BVDV RNA, a reaction efficiency of 103.2%, a correlation coefficient (R²) of 0.995, and a maximum intra-assay CV of 2.63%. It was 10-fold more sensitive than conventional RT-PCR and can quantitatively detect BVDV RNA levels from 10-fold serial dilutions of titrated viruses containing a titer from 10^-1to 10^-5TCID₅₀, without non-specific amplification. Melting curve analysis showed no primer-dimers and non-specific products.</p> <p>Conclusions</p> <p>The one-step SYBR Green I RT-PCR is specific, sensitive and reproducible for the quantification of BVDV in cell culture. This one-step SYBR Green I RT-PCR strategy may be further optimized as a reliable assay for diagnosing and monitoring BVDV infection in animals. It may also be applied to evaluate candidate agents against HCV using BVDV cell culture model.</p

Development of one-step SYBR Green real-time RT-PCR for quantifying bovine viral diarrhea virus type-1 and its comparison with conventional RT-PCR

Background Bovine viral diarrhea virus (BVDV) is a worldwide pathogen in cattle and acts as a surrogate model for hepatitis C virus (HCV). One-step real-time fluorogenic quantitative reverse transcription polymerase chain reaction (RT-PCR) assay based on SYBR Green I dye has not been established for BVDV detection. This study aims to develop a quantitative one-step RT-PCR assay to detect BVDV type-1 in cell culture. Results One-step quantitative SYBR Green I RT-PCR was developed by amplifying cDNA template from viral RNA and using in vitro transcribed BVDV RNA to establish a standard curve. The assay had a detection limit as low as 100 copies/ml of BVDV RNA, a reaction efficiency of 103.2%, a correlation coefficient (R2) of 0.995, and a maximum intra-assay CV of 2.63%. It was 10-fold more sensitive than conventional RT-PCR and can quantitatively detect BVDV RNA levels from 10-fold serial dilutions of titrated viruses containing a titer from 10-1 to 10-5 TCID50, without non-specific amplification. Melting curve analysis showed no primer-dimers and non-specific products. Conclusions The one-step SYBR Green I RT-PCR is specific, sensitive and reproducible for the quantification of BVDV in cell culture. This one-step SYBR Green I RT-PCR strategy may be further optimized as a reliable assay for diagnosing and monitoring BVDV infection in animals. It may also be applied to evaluate candidate agents against HCV using BVDV cell culture model

Investigating characteristics of the long-term settlement of railway embankments in warm permafrost areas

Introduction: The embankment in the permafrost zone of the Qinghai–Tibet Railway (QTR) faces the problem of permafrost degradation, especially in the warm and ice-rich permafrost areas. The settlement deformation of the embankment is more serious in these areas.Methods: This study systematically investigates the settlement deformation characteristics during 16 operational years of three types of typical roadbed structures. The traditional embankment (TE), U-shaped crushed-rock embankment (UCRE), and crushed-rock revetment embankment (CRRE) are the roadbed structures. The long-term monitoring ground temperature and deformation data of the embankment section along the QTR in warm permafrost areas from 2005 to 2020 are utilized in analysis.Results and Discussion: This study focuses on the influence law of the roadbed structure form, shady–sunny slope effect, and temperature field change on the settlement of the roadbed. The results indicated that the two types of the crushed-rock embankment (CRE) of the long-term cumulative settlement are less than 50% of the cumulative settlement of the TE, and the impact on controlling the settlement is significant. The annual settlements of the three types of embankment structures are related to the artificial permafrost table (APT) and influenced by cyclical climate change at the regional scale. The annual growth rate of the settlement at the left and right shoulders of the UCRE as a result of the effect of the shady–sunny slope does not vary considerably as the number of operational years increases. The impact of the shady–sunny slope on the CRRE for the various settlements before 2008 was negligible. After 2008, the thermal disturbance to the embankment temperature field induced by the preconstruction and the effect of shady–sunny slopes decreased gradually as the number of operational years increased. In some years of operation, a thawed interlayer in the TE and CRRE greatly affected the embankment settlement acceleration. The settlement growth rate of the TE is related to the decline of the artificial permafrost table (APT). During the operational years, there was no thawed interlayer in the UCRE. The development of the settlement rate is unaffected by the temperature field for either the left or right embankment shoulder

How to achieve bidirectional zero-knowledge authentication?

Due to the completeness, reliability and zero-knowledge nature, the zero-knowledge proof is widely used to designed various protocols, including zero-knowledge authentication protocols. However, the existing zero-knowledge proof scheme cannot realize bidirectional authentication. In this paper, we design a series of bidirectional zero-knowledge protocols based on two new flavors of operations applicable to multiplicative cyclic group. The two notions are formally defined in this paper. We also provide some formal definitions and properties for the two notions. According to our definitions, any bounded polynomial function defined on multiplicative cyclic group has duality and mirror. Based on the two operations, we introduce and formally define dual commitment scheme and mirror commitment scheme. Besides, we provide two efficient constructions for dual commitment and mirror commitment respectively based on CDH assumption and RSA assumption, and named DCCDH, DCRSA, MCCDH and MCRSA respectively. We also provide the extended version supporting multiple messages in the appendix. Then, we design some efficient non-interactive as well as interactive zero-knowledge authentication protocols based on these commitments. The protocols allow two participants to submit commitments to each other so that they can achieve mutual zero-knowledge authentication only a communication initialization needed. Moreovere , similar to other commitment schemes, our schemes also can be widely used to construction of other schemes for cryptography, such as, verifiable secret sharing, zero-knowledge sets, credentials and content extraction signatures

Impact of Brain Injury on Processing of Emotional Prosodies in Neonates

Being able to appropriately process different emotional prosodies is an important cognitive ability normally present at birth. In this study, we used event-related potential (ERP) to assess whether brain injury impacts the ability to process different emotional prosodies (happy, fear, and neutral) in neonates; whether the ERP measure has potential value for the evaluation of neurodevelopmental outcome in later childhood. A total of 42 full-term neonates were recruited from the neonatology department of Peking University First Hospital from June 2014 to January 2015. They were assigned to the brain injury group (n = 20) or control group (n = 22) according to their clinical manifestations, physical examinations, cranial images and routine EEG outcomes. Using an oddball paradigm, ERP data were recorded while subjects listened to happy (20%, deviation stimulus), fearful (20%, deviation stimulus) and neutral (80%, standard stimulus) prosodies to evaluate the potential prognostic value of ERP indexes for neurodevelopment at 30 months of age. Results showed that while the mismatch responses (MMRs) at the frontal lobe were larger for fearful than happy prosody in control neonates, this difference was not observed in neonates with brain injuries. This finding suggests that perinatal brain injury may influence the cognitive ability to process different emotional prosodies in neonatal brain; this deficit could be reflected by decreased MMR amplitudes in response to fearful prosody. Moreover, the decreased MMRs at the frontal lobe was associated with impaired neurodevelopment at 30 months old

Research progress on extraction, purification, structure and biological activity of Dendrobium officinale polysaccharides

Dendrobium officinale Kimura et Migo (D. officinale) is a traditional medicinal and food homologous plant that has been used for thousands of years in folk medicine and nutritious food. Recent studies have shown that polysaccharide is one of the main biologically active components in D. officinale. D. officinale polysaccharides possess several biological activities, such as anti-oxidant, heptatoprotective, immunomodulatory, gastrointestinal protection, hypoglycemic, and anti-tumor activities. In the past decade, polysaccharides have been isolated from D. officinale by physical and enzymatic methods and have been subjected to structural characterization and activity studies. Progress in extraction, purification, structural characterization, bioactivity, structure-activity relationship, and possible bioactivity mechanism of polysaccharides D. officinale were reviewed. In order to provide reference for the in-depth study of D. officinale polysaccharides and the application in functional food and biomedical research

Responses of carbon exchange characteristics to meteorological factors, phenology, and extreme events in a rubber plantation of Danzhou, Hainan: evidence based on multi-year data

IntroductionOn Hainan Island, a rubber plantation that occupies a large swath of land plays an important role in the regional carbon budget. However, the carbon exchange of the rubber plantation is poorly understood.MethodsIn this study, using the eddy covariance methods we measured carbon metrics in the rubber plantation for 13 years from 2010 to 2022.ResultsWe clarified that the rubber plantation is a carbon sink and the annual net ecosystem exchange (NEE), ecosystem respiration, and gross primary production were −911.89 ± 135.37, 1,528.04 ± 253.50, and 2,439.93 ± 259.63 gC·m−2·a−1, respectively. Carbon fluxes differed between interannual years; specifically, rainy season fluxes were nearly double dry season fluxes. Radiation explained 46% of the variation for NEE in rainy season, and temperature explained 36% of the variation for NEE in the dry season. LAI explained the highest proportion of the monthly variation in NEE (R2 = 0.72, p &lt; 0.001), indicating that when hydrothermal conditions are sufficient phenology may be the primary factor controlling carbon sequestration of rubber plantation. Due to climate change, there is an increasing probability of extreme climate events, such as typhoons, heat waves, and drought. Thus, we compared NEE before and after such events and results show extreme climate events reduce carbon uptake in the rubber plantation. We found that typhoons reduced NEE to varying degrees on different timescales. Heat waves generally decreased NEE during the day but recovered quickly and increased carbon uptake if there was sufficient precipitation. Drought reduced carbon uptake and continued to decrease even after precipitation.DiscussionEstimating the carbon sink capacity of the rubber plantation and studying the response to regional environmental changes are important for both applied research (carbon sink research and market trading, sink enhancement, and emission reduction, etc.) and basic research (land use change, phenology change, etc.)