Bis(2-bromo­acetato-κ2 O,O′)(1,10-phenanthroline-κ2 N,N′)copper(II)

The two halves of the title compound, [Cu(C2H2BrO2)2(C12H8N2)], are related by twofold symmetry along the b axis through the central CuII ion. The CuII ion is coordinated by two symmetry-related N atoms from the 1,10-phenanthroline ligand and four O atoms from two 2-bromo­acetate ligands, showing a distorted octahedral geometry. Weak inter­molecular C—H⋯O inter­actions link neighbouring mol­ecules

Numerical investigation on flow-induced structural vibration and noise in centrifugal pump

A full scale structural vibration and noise induced by flow was simulated by a hybrid numerical method. An interior flow field was solved by large eddy simulation firstly. The sliding mesh technique was applied to take into account the impeller-volute interaction. A sensitivity analysis on effects of near-wall grid size and sampling time on amplitude of pressure pulsations was performed to impose appropriate vibration exciting source. Computed modal of pump components was validated by experimental results, before the volute vibration and sound field were simulated using a coupled vibro-acoustic model. The numerical results indicated that the amplitude of pressure fluctuation, especially on those points located at near the volute tongue, strongly depended on near-wall grid size. The dominated frequency of the vibration velocity of volute was also blade-passing frequency (BPF), which was in according with frequency spectral characteristics of unsteady pressure fluctuation. Directivity distribution of radiation acoustic field excited by volute vibration was typical dipoles. This study shows that it is feasible to use the hybrid numerical method to evaluate the flow-induced vibration and noise generated in centrifugal pump

Identification of novel gene signature for lung adenocarcinoma by machine learning to predict immunotherapy and prognosis

BackgroundLung adenocarcinoma (LUAD) as a frequent type of lung cancer has a 5-year overall survival rate of lower than 20% among patients with advanced lung cancer. This study aims to construct a risk model to guide immunotherapy in LUAD patients effectively.Materials and methodsLUAD Bulk RNA-seq data for the construction of a model, single-cell RNA sequencing (scRNA-seq) data (GSE203360) for cell cluster analysis, and microarray data (GSE31210) for validation were collected from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) database. We used the Seurat R package to filter and process scRNA-seq data. Sample clustering was performed in the ConsensusClusterPlus R package. Differentially expressed genes (DEGs) between two groups were mined by the Limma R package. MCP-counter, CIBERSORT, ssGSEA, and ESTIMATE were employed to evaluate immune characteristics. Stepwise multivariate analysis, Univariate Cox analysis, and Lasso regression analysis were conducted to identify key prognostic genes and were used to construct the risk model. Key prognostic gene expressions were explored by RT-qPCR and Western blot assay.ResultsA total of 27 immune cell marker genes associated with prognosis were identified for subtyping LUAD samples into clusters C3, C2, and C1. C1 had the longest overall survival and highest immune infiltration among them, followed by C2 and C3. Oncogenic pathways such as VEGF, EFGR, and MAPK were more activated in C3 compared to the other two clusters. Based on the DEGs among clusters, we confirmed seven key prognostic genes including CPA3, S100P, PTTG1, LOXL2, MELTF, PKP2, and TMPRSS11E. Two risk groups defined by the seven-gene risk model presented distinct responses to immunotherapy and chemotherapy, immune infiltration, and prognosis. The mRNA and protein level of CPA3 was decreased, while the remaining six gene levels were increased in clinical tumor tissues.ConclusionImmune cell markers are effective in clustering LUAD samples into different subtypes, and they play important roles in regulating the immune microenvironment and cancer development. In addition, the seven-gene risk model may serve as a guide for assisting in personalized treatment in LUAD patients

Ultrasound-guided microwave ablation in the treatment of recurrent primary hyperparathyroidism in a patient with MEN1: a case report

BackgroundMultiple endocrine neoplasia type 1 (MEN1) is an inherited endocrine syndrome caused by the mutation in the tumor suppressor gene MEN1. The recurrence rate of primary hyperparathyroidism (PHPT) in patients with MEN1 after parathyroidectomy remains high, and the management of recurrent hyperparathyroidism is still challenging.Case presentationWe reported a 44-year-old woman with MEN1 combined with PHPT who was diagnosed through genetic screening of the patient and her family members. After parathyroidectomy to remove one parathyroid gland, the patient suffered from persistent high levels of serum calcium and parathyroid hormone, which returned to normal at up to 8 months after ultrasound-guided microwave ablation (MWA) for bilateral parathyroid glands, suggesting an acceptable short-term prognosis.ConclusionUltrasound-guided MWA for parathyroid nodules may be an effective therapeutic strategy for recurrent PHPT in MEN1 patients

Analysis of sea level variability and its contributions in the Bohai, Yellow Sea, and East China Sea

Understanding the sea level variability of the Bohai, Yellow Sea, and East China Sea (BYECS) is crucial for the socio-cultural and natural ecosystems of the coastal regions. In this study, based on satellite altimetry data, selected time range from 1993 to 2020, using the cyclostationary empirical orthogonal function (CSEOF) analysis method distinguishes the primary sea level variability modes. The analysis encompasses the seasonal signal, trend, and El Niño-Southern Oscillation (ENSO) associated mode of sea level anomaly. The amplitude of the annual cycle demonstrates a non-stationary signal, fluctuating between -15% and 15% from the average. Monsoons, atmospheric forcing, ocean circulation, wind-driven Ekman transport, and the Kuroshio emerge as the primary factors influencing BYECS variability on seasonal scales. The satellite altimetry sea level exhibits an average trend within the range of 3-4 mm/year, while the steric sea level trend is generally smaller, falling within the range of 0-2 mm/year. Throughout the entire period, the contribution of steric sea level to the mean sea level trend consistently remains below 25%. Furthermore, BYECS sea level variations have a sensitive response to strong El Niño years, with a clear regionalization of the response, which is related to the intricate atmospheric circulation and local wind pressures, as well as the influence of ocean circulation. In conclusion, we gained a more comprehensive understanding of sea level variability in the BYECS, especially the annual cycle of sea level amplitude and the response of ENSO. However, more studies still need to be done to differentiate the various factors in sea level variations

The Beam Screening Device for the Production of Radioisotopes Based on 100 MeV High-Flow Proton Cyclotron

In some radioisotope production experiments，100 MeV high-current proton cyclotron is required to extract a single-turn beam to meet the experimental requirements，but at present，there is a serious problem of turn overlap when the beam is extracted，and a beam screening device needs to be designed to improve the quality of the drawn beam. The beam screening device has a frame structure and slit motion system based on the cyclotron，and a control system based on PLC design to control the stepper motor and use the electronic ruler and PID control algorithm to achieve closed-loop control. After many times of operation and debugging，the motion accuracy of the mechanical device is ±0.2 mm，and the beam screening can be realized during the operation of the accelerator，which has reference significance for the design and manufacture of the cyclotron beam screening device

Degradation of Potassium Rock by Earthworms and Responses of Bacterial Communities in Its Gut and Surrounding Substrates after Being Fed with Mineral

BACKGROUND: Earthworms are an ecosystem's engineers, contributing to a wide range of nutrient cycling and geochemical processes in the ecosystem. Their activities can increase rates of silicate mineral weathering. Their intestinal microbes usually are thought to be one of the key drivers of mineral degradation mediated by earthworms,but the diversities of the intestinal microorganisms which were relevant with mineral weathering are unclear. METHODOLOGY/PRINCIPAL FINDINGS: In this report, we show earthworms' effect on silicate mineral weathering and the responses of bacterial communities in their gut and surrounding substrates after being fed with potassium-bearing rock powder (PBRP). Determination of water-soluble and HNO(3)-extractable elements indicated some elements such as Al, Fe and Ca were significantly released from mineral upon the digestion of earthworms. The microbial communities in earthworms' gut and the surrounding substrates were investigated by amplified ribosomal DNA restriction analysis (ARDRA) and the results showed a higher bacterial diversity in the guts of the earthworms fed with PBRP and the PBRP after being fed to earthworms. UPGMA dendrogram with unweighted UniFrac analysis, considering only taxa that are present, revealed that earthworms' gut and their surrounding substrate shared similar microbiota. UPGMA dendrogram with weighted UniFrac, considering the relative abundance of microbial lineages, showed the two samples from surrounding substrate and the two samples from earthworms' gut had similarity in microbial community, respectively. CONCLUSIONS/SIGNIFICANCE: Our results indicated earthworms can accelerate degradation of silicate mineral. Earthworms play an important role in ecosystem processe since they not only have some positive effects on soil structure, but also promote nutrient cycling of ecosystem by enhancing the weathering of minerals

Influence of packing density and surface roughness of vertically-aligned carbon nanotubes on adhesive properties of gecko-inspired mimetics.

We have systematically studied the macroscopic adhesive properties of vertically aligned nanotube arrays with various packing density and roughness. Using a tensile setup in shear and normal adhesion, we find that there exists a maximum packing density for nanotube arrays to have adhesive properties. Too highly packed tubes do not offer intertube space for tube bending and side-wall contact to surfaces, thus exhibiting no adhesive properties. Likewise, we also show that the surface roughness of the arrays strongly influences the adhesion properties and the reusability of the tubes. Increasing the surface roughness of the array strengthens the adhesion in the normal direction, but weakens it in the shear direction. Altogether, these results allow progress toward mimicking the gecko's vertical mobility.The authors acknowledge funding from the EC project Technotubes.This is the accepted manuscript. The final version is available at http://pubs.acs.org/doi/abs/10.1021/am507822b