From the Other to the Master Narrative : the Chinese Journey of the Frankfurt School

In their article, From the \u27Other\u27 to the \u27Master Narrative\u27: The Chinese Journey of the Frankfurt School, Guohua Zhu and Xiangchun Meng discuss the Chinese reception of Frankfurt School and the Maoist historical context. Chinese scholars take the narrow view of the Frankfurt School theories as a depoliticized instrument to explain Chinese practice, particularly in the realm of mass culture. Furthermore, the Frankfurt School has encountered the powerful political and ideological legacy of Maoism, which not only dictates instrumentalist view, but also predisposes to a nationalistic attitude that pits Chinese exceptionalism against universalism, including the Frankfurt School and other western Marxist theories. It is thus imperative to disentangle the complex relationship of Critical Theory and Maoism in China. Recontexualizing the broader history of Chinese reception and response to western theories and knowledge will help renew conversations with the West in humanities and social sciences

A novel esterase gene cloned from a metagenomic library from neritic sediments of the South China Sea

<p>Abstract</p> <p>Background</p> <p>Marine microbes are a large and diverse group, which are exposed to a wide variety of pressure, temperature, salinity, nutrient availability and other environmental conditions. They provide a huge potential source of novel enzymes with unique properties that may be useful in industry and biotechnology. To explore the lipolytic genetic resources in the South China Sea, 23 sediment samples were collected in the depth < 100 m marine areas.</p> <p>Results</p> <p>A metagenomic library of South China Sea sediments assemblage in plasmid vector containing about 194 Mb of community DNA was prepared. Screening of a part of the unamplified library resulted in isolation of 15 unique lipolytic clones with the ability to hydrolyze tributyrin. A positive recombinant clone (pNLE1), containing a novel esterase (Est_p1), was successfully expressed in <it>E. coli </it>and purified. In a series of assays, Est_p1 displayed maximal activity at pH 8.57, 40°C, with ρ-Nitrophenyl butyrate (C₄) as substrate. Compared to other metagenomic esterases, Est_p1 played a notable role in specificity for substrate C₄(<it>k</it>_cat/<it>K</it>_mvalue 11,500 S^-1m M^-1) and showed no inhibited by phenylmethylsulfonyl fluoride, suggested that the substrate binding pocket was suitable for substrate C₄and the serine active-site residue was buried at the bottom of substrate binding pocket which sheltered by a lid structure.</p> <p>Conclusions</p> <p>Esterase, which specificity towards short chain fatty acids, especially butanoic acid, is commercially available as potent flavoring tools. According the outstanding activity and specificity for substrate C₄, Est_p1 has potential application in flavor industries requiring hydrolysis of short chain esters.</p

A microencapsulation approach to design microbial seed coatings to boost wheat seed germination and seedling growth under salt stress

IntroductionSalt stress in seed germination and early seedling growth is the greatest cause of crop loss in saline-alkali soils. Microbial seed coating is an effective way to promote plant growth and salt resistance, but these coatings suffer from poor seed adhesion and low survival rates under typical storage conditions.MethodsIn this study, the marine bacterium Pontibacter actiniarum DSM 19842 from kelp was isolated and microencapsulated with calcium alginate using the emulsion and internal gelation method.ResultsCompared to unencapsulated seeds, the spherical microcapsules demonstrated a bacterial encapsulation rate of 65.4% and survival rate increased by 22.4% at 25°C for 60 days. Under salt stress conditions, the seed germination percentage of microcapsule-embedded bacteria (M-Embed) was 90%, which was significantly increased by 17% compared to the germination percentage (73%) of no coating treatment (CK). Root growth was also significantly increased by coating with M-Embed. Chlorophyll, peroxidase, superoxide dismutase, catalase, proline, hydrogen peroxide and malondialdehyde levels indicated that the M-Embed had the best positive effects under salt stress conditions.DiscussionTherefore, embedding microorganisms in suitable capsule materials provides effective protection for the survival of the microorganism and this seed coating can alleviate salt stress in wheat. This process will benefit the development of sustainable agriculture in coastal regions with saline soils

Influence of synthetic superparamagnetic iron oxide on dendritic cells

Yongbin Mou1, Baoan Chen2, Yu Zhang3, Yayi Hou4, Hao Xie4, Guohua Xia2, Meng Tang5, Xiaofeng Huang1, Yanhong Ni1, Qingang Hu1,6 1Central Laboratory of Stomatology, Stomatological Hospital Affiliated Medical School, Nanjing University, 2Department of Hematology, Zhongda Hospital, Medical School, Southeast University, 3State Key Laboratory of Bioelectronics, Jiangsu Key Laboratory for Biomaterials and Devices, Southeast University, 4Immunology and Reproductive Biology Laboratory, Medical School, Nanjing University, 5Laboratory of Environmental Medicine and Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing, People&amp;#39;s Republic of China; 6Leeds Dental Institute, Faculty of Medicine and Health, University of Leeds, Leeds, UK Background: This study investigated the influence of synthetic superparamagnetic iron oxide (SPIO) on dendritic cells and provides a possible method for labeling these cells. Methods: SPIO nanoparticles were prepared, and their morphology and magnetic properties were characterized. The particles were endocytosed by dendritic cells generated from mouse bone marrow. Labeling efficiency and cellular uptake were analyzed by Prussian blue staining and quantitative spectrophotometric assay. Meanwhile, the surface molecules, cellular apoptosis, and functional properties of the SPIO-labeled dendritic cells were explored by flow cytometry and the mixed lymphocyte reaction assay. Results: The synthetic nanoparticles possessed a spherical shape and good superparamagnetic behavior. The mean concentration of iron in immature and mature dendritic cells was 31.8 &amp;plusmn; 0.7 &amp;micro;g and 35.6 &amp;plusmn; 1.0 &amp;micro;g per 1 &amp;times; 106 cells, respectively. After 12 hours of incubation with SPIO at a concentration of 25 &amp;micro;g/mL, nearly all cells were shown to contain iron. Interestingly, cellular apoptosis and surface expression of CD80, CD86, major histocompatibility II, and chemokine receptor 7 in mature dendritic cells were not affected to any significant extent by SPIO labeling. T cell activation was maintained at a low ratio of dendritic cells to T cells. Conclusion: SPIO nanoparticles have good superparamagnetic behavior, highly biocompatible characteristics, and are suitable for use in further study of the migratory behavior and biodistribution of dendritic cells in vivo. Keywords: superparamagnetic iron oxide, dendritic cell, cell labelin