Substrate entering and product leaving trajectories predict an engulfing dynamic for the major conformational change of the β-lactam acylase

It is still a major challenge to acquire insight into the conformational changes between the ground state and the transition state of an enzyme, although conformational fluctuation within interconverting conformers has been widely investigated (1-4). Here, we utilize different enzymatic reactions in b-lactam acylase to figure out the substrate/product trajectories in the enzyme, thereby probing the overall conformational changes in transition state. First, an auto-proteolytic intermediate of cephalosporin acylase (EC 3.5.1.11) with partial spacer segment was identified. As a final proteolytic step, the deletion of this spacer segment was revealed to be a first-order reaction, suggesting an intramolecular Ntn mechanism for the auto-proteolysis. Accordingly, the different proteolytic sites in the acylase precursor indicate a substrate entering pathway along the spacer peptide. Second, bromoacyl-7ACA can interact with penicillin G acylase (EC 3.5.1.11) in two distinguish aspects, to be hydrolyzed as a substrate analogue and to affinity alkylate the conserved Trpb4 as a product analogue. The kinetic correlation between these two reactions suggests a channel opening from Serb1 to Trpb4, responsible for the main product leaving. These two reaction trajectories relaying at the active centre, together with the crystal structures (5-10), predict an engulfing dynamic involving pocket constriction and channel opening

Analysis of Unemployment During Transition to a Market Economy:

Using self-gathered data and the hazard model, we analyzed the reasons laid-off workers in the Beijing area remained unemployed. For the laid-off workers who were eventually reemployed, we used the Tobit model to explain the change in wages between their pre- and post-layoff employment. The results of these analyses revealed that, although China’s policy of laying off workers from state-owned enterprises without completely severing the relationship accelerated the establishment of a labor market to some extent, with prolonged time the laid-off workers are out of work, the policy was an obstacle to the labor-marketing effort. Moreover, it was an obstacle to implementing and improving an unemployment benefits system. They also showed that laid-off workers with worked experience under the traditional system need to be reeducated to enable them to adapt to the new economic environment as well as to be taught new skills, especially those workers who were managers. Additionally, the "lay-off" is not only a policy in special phase during the process of Chinese economic system reform, but also a special phenomenon, which is the structural unemployment revealed from Chinese economic system. This unemployment due to changes in industry structure means that the government must adopt a more active employment policy

Multipartite entanglement purification with quantum nondemolition detectors

We present a scheme for multipartite entanglement purification of quantum systems in a Greenberger-Horne-Zeilinger state with quantum nondemolition detectors (QNDs). This scheme does not require the controlled-not gates which cannot be implemented perfectly with linear optical elements at present, but QNDs based on cross-Kerr nonlinearities. It works with two steps, i.e., the bit-flipping error correction and the phase-flipping error correction. These two steps can be iterated perfectly with parity checks and simple single-photon measurements. This scheme does not require the parties to possess sophisticated single photon detectors. These features maybe make this scheme more efficient and feasible than others in practical applications.Comment: 8 pages, 5 figure

Selective AP-sequence Based Indoor Localization without Site Survey

In this paper, we propose an indoor localization system employing ordered sequence of access points (APs) based on received signal strength (RSS). Unlike existing indoor localization systems, our approach does not require any time-consuming and laborious site survey phase to characterize the radio signals in the environment. To be precise, we construct the fingerprint map by cutting the layouts of the interested area into regions with only the knowledge of positions of APs. This can be done offline within a second and has a potential for practical use. The localization is then achieved by matching the ordered AP-sequence to the ones in the fingerprint map. Different from traditional fingerprinting that employing all APs information, we use only selected APs to perform localization, due to the fact that, without site survey, the possibility in obtaining the correct AP sequence is lower if it involves more APs. Experimental results show that, the proposed system achieves localization accuracy < 5m with an accumulative density function (CDF) of 50% to 60% depending on the density of APs. Furthermore, we observe that, using all APs for localization might not achieve the best localization accuracy, e.g. in our case, 4 APs out of total 7 APs achieves the best performance. In practice, the number of APs used to perform localization should be a design parameter based on the placement of APs.Comment: VTC2016-Spring, 15-18 May 2016, Nanjing, Chin