Antimicrobial resistant <i>Escherichia coli</i> isolates containing class 1 integrons with the <i>dfrA1-aadA1</i>cassette array.

<p>The isolates were recovered from humans with blood stream infections.</p><p>^aTransferability of plasmids with class 1 integron by conjugation</p><p>^bPBRT = Plasmid-based replicontyping (plasmid replicon(s) in transconjugant); + = transconjugants</p><p>were obtained, ÷ = no transconjugantes were obtained</p><p>^cSubtyping of conjugative incF plasmid with class 1 integron</p><p>ut = unique type, one isolate with this banding pattern was found</p><p>Antimicrobial resistant <i>Escherichia coli</i> isolates containing class 1 integrons with the <i>dfrA1-aadA1</i>cassette array.</p

Antimicrobial resistant <i>Escherichia coli</i> isolates containing class 1 integrons with an <i>aadA1</i> cassette as the only inserted gene cassette.

<p>The isolates were recovered from Norwegian produced meat.</p><p>^aTransferability of plasmids with class 1 integron by conjugation; + = transconjugants were obtained, ÷ = no transconjugantes were obtained</p><p>^bPBRT = Plasmid-based replicontyping (plasmid replicon(s) in transconjugant)</p><p>Antimicrobial resistant <i>Escherichia coli</i> isolates containing class 1 integrons with an <i>aadA1</i> cassette as the only inserted gene cassette.</p

Primers used in the PCR experiments.

<p>*the aadaI primer was used in combination with the 5’-CS primer for amplification of class 1 integrons lacking the 3’-conserved segment. The other primers were used pairwise as listed.</p><p>Primers used in the PCR experiments.</p

Number of integrons and different cassette arrays occurring in antimicrobial resistant <i>Escherichia coli</i> originating from humans with blood stream infections and in antimicrobial resistant <i>E</i>. <i>coli</i> originating from meat and meat products.

<p>^aClass 1 integron lacking the 3- conserved segment</p><p>^bOne <i>dfrA1</i> cassette is located as the first cassette, the remaining cassettes (if any) are not characterized</p><p>^c The integrons in <i>E</i>. <i>coli</i> from meat and in one human strain (with <i>dfrA12-orfF-aadA2</i> cassettes) have been characterized previously [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0128797#pone.0128797.ref001" target="_blank">1</a>,<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0128797#pone.0128797.ref030" target="_blank">30</a>].</p><p>Number of integrons and different cassette arrays occurring in antimicrobial resistant <i>Escherichia coli</i> originating from humans with blood stream infections and in antimicrobial resistant <i>E</i>. <i>coli</i> originating from meat and meat products.</p

Minimum spanning tree analysis based on 50 multilocus sequence types (STs) in 248 chlamydia specimens.

<p>The 50 circles correspond to different STs discriminated by multilocus sequence typing (MLST). Each circle represents an ST, and ST number is given inside or next to the circle. Circle size reflects the number of isolates. Bold black lines connect single-locus variants (SLV). Broken lines connecting double-locus variants are only indicative as several alternative links with equal weight may exist. The coloured pie charts indicate ST geographic distribution. Grey shaded areas define clonal complexes i.e. clusters of genetically related STs with only one allele difference.</p

Geographic distribution of <i>C. trachomatis</i> specimens according to three different strain typing methods, and genetic diversity within each area.

<p>CI: Confidence interval.</p>1<p><i>C. trachomatis</i> specimens from either the high school study (n = 60) or routine clinical samples in the laboratory (n = 20).</p>2<p>The numbers reflect the results for all 248 specimens and do not necessarily equal the sum of each row.</p>3<p>Sequence types of <i>C. trachomatis</i> detected by multilocus sequence typing.</p>4<p>Number of STs identified in an area divided by number of chlamydia specimens in the area.</p>5<p>Percentage novel STs in an area of total number of STs in the area.</p>6<p>Genovar D–K of <i>C. trachomatis</i> inferred from <i>ompA</i> sequencing.</p>7<p>Genotypes of <i>C. trachomatis</i> detected by <i>ompA</i> sequencing.</p

Number of genetic variants of <i>ompA</i> sequencing and multilocus sequence typing (MLST) within each genovar (D–K) in 248 <i>C. trachomatis</i> specimens.

1<p>Sequence types (STs) of <i>C. trachomatis</i> detected by MLST.</p>2<p>The numbers reflect the total number of unique genetic variants in the 248 chlamydia specimens and do not equal the sum of each column.</p