A NEW STABILITY INDICATING UPLC METHOD DEVELOPMENT AND VALIDATION FOR THE SIMULTANEOUS ESTIMATION OF METOLAZONE AND SPIRONOLACTONE IN BULK AND IN ITS PHARMACEUTICAL FORMULATIONS

Objective: The objective of the work is to develop and validate a new, simple, highly sensitive RP-UPLC method for simultaneous estimation of Metolazone and Spironolactone in bulk and in its dosage forms. Methods: The method was developed on a reversed-phase Hypersil Gold C18 (2.1× 100 mm, 2.7 µm) column with isocratic elution. Detection was done by UV-Spectroscopy at a detection wavelength of 235 nm. The analytical procedure was validated by assessing the specificity, linearity, precision, accuracy, limit of detection, limit of quantification, robustness and ruggedness as per ICH guidelines. Results: The results were obtained as follows- the retention times were found to be around 2.888 min and 3.835 min, the percentage purity was observed to be 99 % w/v and 100 % w/v, the percentage recovery was found to be 99.90% and 99.9% respectively for Metolazone and Spironolactone. Calibration plots were linear (r2 > 0.999) over the concentration range of 12 to 28μg/ml for Metolazolone and 120 to 280μg/ml for Spironolactone. The LOD was 0.0002µg/ml for Metolazone and 0.01µg/ml for Spironolactone. The LOQ was found to be 0.0008µg/ml for Metolazone and 0.003µg/ml for Spironolactone. Conclusion: The developed analytical method for the simultaneous quantitation of Metolazone and Spironolactone was found to be specific, rapid, reliable, and reproducible. No interference from any component of pharmaceutical dosage form was observed. The method is amenable to the routine analysis of large numbers of samples with good precision and accuracy

Comprehensive analysis of etiology, prognosis and clinical outcome of acute pancreatitis in a tertiary care center

INTRODUCTION: Acute pancreatitis is a common cause of morbidity and mortality in the working population of our society Our study would like to identity the most common cause among our population hence it will help the society in developing preventive strategies for the same. Even though there have been several prognostic scoring systems defined for acute pancreatitis, BISAP and CTSI remain the reliable clinical and radiological tools, respectively. We would like to correlate these scores with the clinical outcome in our tertiary setup which may aid to start the early appropriate treatment strategy. AIMS AND OBJECTIVES: 1. To identify most common etiological agent of acute pancreatitis in our institution. 2. To correlate the existing clinical (BISAP) and radiological (Modified CTSI) prognostic scoring systems in Acute pancreatitis with the clinical outcomes of patients in our institution. MATERIALS AND METHODS: Patients admitted in Rajiv Gandhi Govt. General Hospital, Chennai with Acute pancreatitis as per inclusion and exclusion criteria are subjected to Blood investigations like Complete hemogram, Renal function test, Liver Function Test and USG Abdomen, CECT Abdomen (i.v. contrast) for all cases. The BISAP and modified CT severity index scoring system is then applied and individual scores of patients are calculated. The patient is subsequently followed up and the clinical course is observed. Clinical outcome in terms of Length of hospital stay, requirement of ICU observation, complications and mortality are observed. All collected data will be analyzed and conclusions derived. Sample Size: 50 Patients. Study design: Prospective and retrospective study. Period of Study: October 2016 – September 2017. Setting: Institute of General Surgery, Rajiv Gandhi Govt. General Hospital. The study was conducted after obtaining the Institutional Ethical Committee approval. Inclusion Criteria: 1. Patients with a clinical picture consistent with the diagnosis of acute pancreatitis, along with radiological evidence of inflamed pancreas will be considered to have acute pancreatitis. 2. First episode of Acute Pancreatitis. 3. Age > 18 years and Age < 70 years. Exclusion Criteria: 1. Proven cases of chronic pancreatitis. 2. Hereditary pancreatitis. 3. Acute pancreatitis patients with organ failure at or within 24hrs of presentation. 4. Pregnancy. 5. Chronic kidney disease. 6. Taumatic pancreatitis with head injury. 7. Mental retardation. CONCLUSION: 1. Alcohol is the most common etiological factor for acute pancreatitis in productive young population. 2. The BISAP score is more accurate in predicting disease severity and significantly than CTSI in this study. 3. With this study, we conclude that the BISAP score is the simple, bedside and accurate clinical scoring system for the prediction of disease severity in acute pancreatitis. Hence early diagnosis and initiation of treatment at appropriate center can be planned to reduce the adverse outcome

19-Ferrocenyl-18-oxa-8,16-diaza­penta­cyclo­[8.6.3.01,10.02,7.012,16]nona­deca-2(7),3,5-triene-9,17-dione

In the title compound, [Fe(C5H5)(C21H19N2O3)], both pyrrol­idine rings of the pyrrolizine substructure show an envelope conformation. In the ferrocenyl moiety, the unsubstituted cyclo­penta­dienyl ring is disordered over two orientations with site occupancies of 0.64 (2) and 0.36 (2). In the pyrrolizine ring, one C atom is disordered over two positions, with site occupancies of 0.71 (1) and 0.29 (1). Intra­molecular C—H⋯O inter­actions occur. The crystal packing is established through weak inter­molecular C—H⋯O and N—H⋯O inter­actions

4′-Ferrocenyl-1′-methylacenapthylene-1-spiro-2′-pyrrolidine-3′-spiro-2′′-indane-2,1′′,3′′(1H)-trione

In the title compound, [Fe(C5H5)(C29H20NO3)], the acenaphthyl­ene ring system makes a dihedral angle of 83.77 (3)° with the indane-1,3-dione ring system. The central pyrrolidine ring exhibits a twist conformation. In the crystal, mol­ecules are linked by a weak inter­molecular C—H⋯O inter­action into a chain along the b axis. Two weak intra­molecular C—H⋯O inter­actions are also present

Methyl 9-p-tolyl-8a,9,9a,10,11,12,13,14a-octa­hydro-8H-benzo[f]chromeno[3,4-b]indolizine-8a-carboxyl­ate

In the title compound, C28H29NO3, the fused pyrrolidine and piperidine rings of the octa­hydro­indolizine unit exhibit envelope and chair conformations, respectively. The dihedral angle between the naphthalene ring system and the benzene ring is 40.37 (5)°. The crystal packing is stabilized by weak inter­molecular C—H⋯O inter­actions

Crystal structure of 3-(morpholin-4-yl)-1-phenyl-3-(pyridin-2-yl)propan-1-one

Acknowledgements FMMA acknowledges the PG and Research Department of Chemistry and the Management of Jamal Mohamed College (Autonomous) for their kind support.Peer reviewedPublisher PD

Vibrio alginolytigus causing shell disease in the mud crab Scylla serrata (Forskal 1775)

1359-1363Scylla serrata is one of the most cultured mud crab species in the aquaculture which is also susceptible to shell disease. In the present study, Vibrio alginolyticus MF680287.1 caused by shell disease and isolated from infected mud crab S. serrata grow out pond located at Mahendrapalli, Nagapattinam District, Tamil Nadu, India. Further, gross observation of infected mud crab showed shell lesion on the dorsal carapace. The histological examination of normal and diseased mud crab S. serrata carapace and gills was conducted. The shell lesion affected in the S. serrata carapace layers showed loss of membrenous layer and epithilium. The bacterial colonies were abundant in the cuticle. The gill lamellae showed cuticlar damage in the formation of haemocyte nodules and eosinophilic granular cells

3-(4-Methoxy­benz­yl)-1-benzothio­phene

In the title compound, C16H14OS, the dihedral angle between the benzothio­phene ring system and the benzene ring is 72.41 (12)°. A weak inter­molecular C—H⋯π inter­action from the benzene ring to the benzothio­phene ring system is observed in the crystal structure