7 research outputs found
ffects of green and black tea biocomposites on endogenous synthesis, metabolism and genotoxic effect of carcinogenic N-nitrosodimethylamine
Aim: To study the modifying effect of green and black tea biocomposites on endogenous synthesis and genotoxic action of the carcinogenic N-nitrosodimethylamine. Methods: Green and black tea biocomposites were administered to the white inbred rats in vivo. Amidopyrine and sodium nitrite were used as N-nitrosodimethylamine precursors and 4-methylpyrazol as an inhibitor of its metabolism. N-nitrosodimethylamine (blood, daily urine and reaction mixture), nitrites and nitrates (daily urine) levels were measured. Genotoxic action was tested by formation of DNA single-strand breaks in hepatocytes. Results: In in vitro system, biocomposites increased N-nitrosodimethylamine synthesis in neutral medium and decreased in acid conditions. In vivo, black tea biocomposite consumption resulted in enhanced background level of DNA single-strand breaks in rats hepatocytes and higher genotoxic effect upon administration of N-nitrosodimethylamine precursors. The levels of N-nitrosodimethylamine in blood and urine of experimental animals were increased after precursorsβ administration. In contrast, green tea biocomposite significantly decreased background level of DNA single-strand breaks. However, there was no protective action of this food supplement at the N-nitrosodimethylamine, precursorsβ administration. 4-methylpyrazol administration did not increase N-nitrosodimethylamine excretion in urine, while this effect was observed in control and black tea biocomposite groups. Conclusions: The effects of green tea and black tea biocomposites on N-nitrosodimethylamine synthesis in in vitro system are unidirectional and depend on biocompositesβ concentration and acidity of the medium. Long-term consumption of black tea biocomposite resulted in intensification of endogenous N-nitrosodimethylamine synthesis and increased damage of the hepatocytesβ DNA. As to the green tea biocomposite, the obtained results allow us to suggest that this biocomposite enhanced N-nitrosodimethylamine metabolism.Π¦Π΅Π»Ρ: ΠΈΠ·ΡΡΠΈΡΡ ΠΌΠΎΠ΄ΠΈΡΠΈΡΠΈΡΡΡΡΠΈΠΉ ΡΡΡΠ΅ΠΊΡ Π±ΠΈΠΎΠΊΠΎΠΌΠΏΠΎΠ·ΠΈΡΠΎΠ² ΠΈΠ· Π·Π΅Π»Π΅Π½ΠΎΠ³ΠΎ ΠΈ ΡΠ΅ΡΠ½ΠΎΠ³ΠΎ ΡΠ°Ρ Π½Π° ΡΠ½Π΄ΠΎΠ³Π΅Π½Π½ΡΠΉ ΡΠΈΠ½ΡΠ΅Π· ΠΈ Π³Π΅Π½ΠΎΡΠΎΠΊΡΠΈΡΠ΅ΡΠΊΠΎΠ΅
Π΄Π΅ΠΉΡΡΠ²ΠΈΠ΅ ΠΊΠ°Π½ΡΠ΅ΡΠΎΠ³Π΅Π½Π½ΠΎΠ³ΠΎ N-Π½ΠΈΡΡΠΎΠ·ΠΎΠ΄ΠΈΠΌΠ΅ΡΠΈΠ»Π°ΠΌΠΈΠ½Π°. ΠΠ΅ΡΠΎΠ΄Ρ: Π² ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ΅ in vivo Π±Π΅Π»ΡΠ΅ Π½Π΅Π»ΠΈΠ½Π΅ΠΉΠ½ΡΠ΅ ΠΊΡΡΡΡ ΠΏΠΎΠ»ΡΡΠ°Π»ΠΈ
Π±ΠΈΠΎΠΊΠΎΠΌΠΏΠΎΠ·ΠΈΡΡ ΠΈΠ· Π·Π΅Π»Π΅Π½ΠΎΠ³ΠΎ ΠΈ ΡΠ΅ΡΠ½ΠΎΠ³ΠΎ ΡΠ°Ρ. ΠΠΌΠΈΠ΄ΠΎΠΏΠΈΡΠΈΠ½ ΠΈ Π½ΠΈΡΡΠΈΡ Π½Π°ΡΡΠΈΡ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π»ΠΈ Π² ΠΊΠ°ΡΠ΅ΡΡΠ²Π΅ ΠΏΡΠ΅Π΄ΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΈΠΊΠΎΠ²
N-Π½ΠΈΡΡΠΎΠ·ΠΎΠ΄ΠΈΠΌΠ΅ΡΠΈΠ»Π°ΠΌΠΈΠ½Π° ΠΈ 4-ΠΌΠ΅ΡΠΈΠ»ΠΏΠΈΡΠ°Π·ΠΎΠ» Π² ΠΊΠ°ΡΠ΅ΡΡΠ²Π΅ ΠΈΠ½Π³ΠΈΠ±ΠΈΡΠΎΡΠ° Π΅Π³ΠΎ ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΠΈΠ·ΠΌΠ°. ΠΠ·ΠΌΠ΅ΡΡΠ»ΠΈ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅ N-Π½ΠΈΡΡΠΎΠ·ΠΎΠ΄ΠΈΠΌΠ΅ΡΠΈΠ»Π°ΠΌΠΈΠ½Π°
(ΠΊΡΠΎΠ²Ρ, ΡΡΡΠΎΡΠ½Π°Ρ ΠΌΠΎΡΠ° ΠΈ ΡΠ΅Π°ΠΊΡΠΈΠΎΠ½Π½Π°Ρ ΡΠΌΠ΅ΡΡ), Π½ΠΈΡΡΠΈΡΠΎΠ² ΠΈ Π½ΠΈΡΡΠ°ΡΠΎΠ² (ΡΡΡΠΎΡΠ½Π°Ρ ΠΌΠΎΡΠ°). ΠΠ΅Π½ΠΎΡΠΎΠΊΡΠΈΡΠ΅ΡΠΊΠΎΠ΅ Π΄Π΅ΠΉΡΡΠ²ΠΈΠ΅
ΠΎΡΠ΅Π½ΠΈΠ²Π°Π»ΠΈ ΠΏΠΎ ΠΎΠ±ΡΠ°Π·ΠΎΠ²Π°Π½ΠΈΡ ΠΎΠ΄Π½ΠΎΠ½ΠΈΡΠ΅Π²ΡΡ
ΡΠ°Π·ΡΡΠ²ΠΎΠ² ΠΠΠ Π² Π³Π΅ΠΏΠ°ΡΠΎΡΠΈΡΠ°Ρ
. Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ: Π² ΡΠΈΡΡΠ΅ΠΌΠ΅ in vitro Π±ΠΈΠΎΠΊΠΎΠΌΠΏΠΎΠ·ΠΈΡΡ ΠΏΠΎΠ²ΡΡΠ°Π»ΠΈ
ΡΠΈΠ½ΡΠ΅Π· N-Π½ΠΈΡΡΠΎΠ·ΠΎΠ΄ΠΈΠΌΠ΅ΡΠΈΠ»Π°ΠΌΠΈΠ½Π° ΠΏΡΠΈ Π½Π΅ΠΉΡΡΠ°Π»ΡΠ½ΠΎΠΉ ΠΊΠΈΡΠ»ΠΎΡΠ½ΠΎΡΡΠΈ ΡΠ΅Π°ΠΊΡΠΈΠΎΠ½Π½ΠΎΠΉ ΡΡΠ΅Π΄Ρ ΠΈ ΡΠ½ΠΈΠΆΠ°Π»ΠΈ Π΅Π³ΠΎ Π² ΠΊΠΈΡΠ»ΡΡ
ΡΡΠ»ΠΎΠ²ΠΈΡΡ
. Π ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ΅
in vivo ΡΠΏΠΎΡΡΠ΅Π±Π»Π΅Π½ΠΈΠ΅ ΠΊΡΡΡΠ°ΠΌΠΈ Π±ΠΈΠΎΠΊΠΎΠΌΠΏΠΎΠ·ΠΈΡΠ° ΠΈΠ· ΡΠ΅ΡΠ½ΠΎΠ³ΠΎ ΡΠ°Ρ ΠΏΡΠΈΠ²ΠΎΠ΄ΠΈΠ»ΠΎ ΠΊ ΠΏΠΎΠ²ΡΡΠ΅Π½ΠΈΡ ΡΡΠΎΠ²Π½Ρ ΠΎΠ΄Π½ΠΎΠ½ΠΈΡΠ΅Π²ΡΡ
ΡΠ°Π·ΡΡΠ²ΠΎΠ²
ΠΠΠ Π² Π³Π΅ΠΏΠ°ΡΠΎΡΠΈΡΠ°Ρ
ΠΈ ΠΏΠΎΠ²ΡΡΠ°Π»ΠΎ Π³Π΅Π½ΠΎΡΠΎΠΊΡΠΈΡΠ΅ΡΠΊΠΈΠΉ ΡΡΡΠ΅ΠΊΡ ΠΎΡ Π²Π²Π΅Π΄Π΅Π½ΠΈΡ ΠΏΡΠ΅Π΄ΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΈΠΊΠΎΠ² N-Π½ΠΈΡΡΠΎΠ·ΠΎΠ΄ΠΈΠΌΠ΅ΡΠΈΠ»Π°ΠΌΠΈΠ½Π°. ΠΠΎΡΠ»Π΅
Π²Π²Π΅Π΄Π΅Π½ΠΈΡ ΠΏΡΠ΅Π΄ΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΈΠΊΠΎΠ² ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅ N-Π½ΠΈΡΡΠΎΠ·ΠΎΠ΄ΠΈΠΌΠ΅ΡΠΈΠ»Π°ΠΌΠΈΠ½Π° Π² ΠΊΡΠΎΠ²ΠΈ ΠΈ ΠΌΠΎΡΠ΅ Π±ΡΠ»ΠΎ ΠΏΠΎΠ²ΡΡΠ΅Π½ΠΎ. ΠΠ°ΠΎΠ±ΠΎΡΠΎΡ, Π±ΠΈΠΎΠΊΠΎΠΌΠΏΠΎΠ·ΠΈΡ
ΠΈΠ· Π·Π΅Π»Π΅Π½ΠΎΠ³ΠΎ ΡΠ°Ρ Π·Π½Π°ΡΠΈΡΠ΅Π»ΡΠ½ΠΎ ΡΠ½ΠΈΠΆΠ°Π» ΡΠΎΠ½ΠΎΠ²ΡΠΉ ΡΡΠΎΠ²Π΅Π½Ρ ΠΎΠ΄Π½ΠΎΠ½ΠΈΡΠ΅Π²ΡΡ
ΡΠ°Π·ΡΡΠ²ΠΎΠ² ΠΠΠ. ΠΠ΄Π½Π°ΠΊΠΎ Π½Π΅ ΠΎΡΠΌΠ΅ΡΠ°Π»ΠΈ Π·Π°ΡΠΈΡΠ½ΠΎΠ³ΠΎ Π΄Π΅ΠΉΡΡΠ²ΠΈΡ
ΡΡΠΎΠΉ ΠΏΠΈΡΠ΅Π²ΠΎΠΉ Π΄ΠΎΠ±Π°Π²ΠΊΠΈ ΠΏΡΠΈ Π²Π²Π΅Π΄Π΅Π½ΠΈΠΈ ΠΏΡΠ΅Π΄ΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΈΠΊΠΎΠ² N-Π½ΠΈΡΡΠΎΠ·ΠΎΠ΄ΠΈΠΌΠ΅ΡΠΈΠ»Π°ΠΌΠΈΠ½Π°. ΠΠ²Π΅Π΄Π΅Π½ΠΈΠ΅ 4-ΠΌΠ΅ΡΠΈΠ»ΠΏΠΈΡΠ°Π·ΠΎΠ»Π° Π½Π΅ ΠΏΡΠΈΠ²ΠΎΠ΄ΠΈΠ»ΠΎ
ΠΊ ΠΏΠΎΠ²ΡΡΠ΅Π½Π½ΠΎΠΉ ΡΠΊΡΠΊΡΠ΅ΡΠΈΠΈ N-Π½ΠΈΡΡΠΎΠ·ΠΎΠ΄ΠΈΠΌΠ΅ΡΠΈΠ»Π°ΠΌΠΈΠ½Π° Ρ ΠΌΠΎΡΠΎΠΉ, Ρ
ΠΎΡΡ ΡΡΠΎΡ ΡΡΡΠ΅ΠΊΡ ΠΎΡΠΌΠ΅ΡΠ°Π»ΠΈ Π² ΠΊΠΎΠ½ΡΡΠΎΠ»ΡΠ½ΠΎΠΉ Π³ΡΡΠΏΠΏΠ΅ ΠΈ Π³ΡΡΠΏΠΏΠ΅ Ρ Π½Π°Π·Π½Π°ΡΠ΅Π½ΠΈΠ΅ΠΌ
Π±ΠΈΠΎΠΊΠΎΠΌΠΏΠΎΠ·ΠΈΡΠ° ΠΈΠ· ΡΠ΅ΡΠ½ΠΎΠ³ΠΎ ΡΠ°Ρ. ΠΡΠ²ΠΎΠ΄Ρ: Π² ΡΠΈΡΡΠ΅ΠΌΠ΅ in vitro Π±ΠΈΠΎΠΊΠΎΠΌΠΏΠΎΠ·ΠΈΡΡ ΠΈΠ· Π·Π΅Π»Π΅Π½ΠΎΠ³ΠΎ ΠΈ ΡΠ΅ΡΠ½ΠΎΠ³ΠΎ ΡΠ°Ρ ΠΎΠ΄Π½ΠΎΠ½Π°ΠΏΡΠ°Π²Π»Π΅Π½ΠΎ
Π²Π»ΠΈΡΠ»ΠΈ Π½Π° ΡΠΈΠ½ΡΠ΅Π· N-Π½ΠΈΡΡΠΎΠ·ΠΎΠ΄ΠΈΠΌΠ΅ΡΠΈΠ»Π°ΠΌΠΈΠ½Π°, Π° ΠΈΡ
ΡΡΡΠ΅ΠΊΡΡ Π·Π°Π²ΠΈΡΠ΅Π»ΠΈ ΠΎΡ ΠΊΠΈΡΠ»ΠΎΡΠ½ΠΎΡΡΠΈ ΡΠ΅Π°ΠΊΡΠΈΠΎΠ½Π½ΠΎΠΉ ΡΡΠ΅Π΄Ρ ΠΈ ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΠΈ Π² Π½Π΅ΠΉ
Π±ΠΈΠΎΠΊΠΎΠΌΠΏΠΎΠ·ΠΈΡΠΎΠ². ΠΠ»ΠΈΡΠ΅Π»ΡΠ½ΠΎΠ΅ ΡΠΏΠΎΡΡΠ΅Π±Π»Π΅Π½ΠΈΠ΅ Π±ΠΈΠΎΠΊΠΎΠΌΠΏΠΎΠ·ΠΈΡΠ° ΠΈΠ· ΡΠ΅ΡΠ½ΠΎΠ³ΠΎ ΡΠ°Ρ ΠΏΡΠΈΠ²ΠΎΠ΄ΠΈΠ»ΠΎ ΠΊ ΠΈΠ½ΡΠ΅Π½ΡΠΈΡΠΈΠΊΠ°ΡΠΈΠΈ ΡΠ½Π΄ΠΎΠ³Π΅Π½Π½ΠΎΠ³ΠΎ ΡΠΈΠ½ΡΠ΅Π·Π°
N-Π½ΠΈΡΡΠΎΠ·ΠΎΠ΄ΠΈΠΌΠ΅ΡΠΈΠ»Π°ΠΌΠΈΠ½Π° ΠΈ ΠΏΠΎΠ²ΡΡΠ°Π»ΠΎ ΡΡΠΎΠ²Π΅Π½Ρ ΠΏΠΎΠ²ΡΠ΅ΠΆΠ΄Π΅Π½ΠΈΠΉ ΠΠΠ Π² Π³Π΅ΠΏΠ°ΡΠΎΡΠΈΡΠ°Ρ
. Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ, ΠΏΠΎΠ»ΡΡΠ΅Π½Π½ΡΠ΅ ΠΎΡΠ½ΠΎΡΠΈΡΠ΅Π»ΡΠ½ΠΎ
Π±ΠΈΠΎΠΊΠΎΠΌΠΏΠΎΠ·ΠΈΡΠ° ΠΈΠ· Π·Π΅Π»Π΅Π½ΠΎΠ³ΠΎ ΡΠ°Ρ, ΠΏΠΎΠ·Π²ΠΎΠ»ΡΡΡ Π½Π°ΠΌ ΠΏΡΠ΅Π΄ΠΏΠΎΠ»ΠΎΠΆΠΈΡΡ, ΡΡΠΎ ΠΎΠ½ ΠΏΠΎΠ²ΡΡΠ°Π» ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΠΈΠ·ΠΌ N-Π½ΠΈΡΡΠΎΠ·ΠΎΠ΄ΠΈΠΌΠ΅ΡΠΈΠ»Π°ΠΌΠΈΠ½Π°
Green tea, red wine and lemon extracts reduce experimental tumor growth and cancer drug toxicity
Aim: To evaluate antitumor effect of plant polyphenol extracts from green tea, red wine lees and/or lemon peel alone and in combination with antitumor drugs on the growth of different transplanted tumors in experimental animals. Materials and Methods: Green tea extract (GTE) was prepared from green tea infusion. GTE-based composites of red wine (GTRW), lemon peel (GTRWL) and/or NanoGTE as well as corresponding nanocomposites were prepared. The total polyphenolics of the different GTE-based extracts ranged from 18.0% to 21.3%. The effects of GTE-based extracts were studied in sarcoma 180, Ehrlich carcinoma, B16 melanoma, Ca755 mammary carcinoma, P388 leukemia, L1210 leukemia, and Guerin carcinoma (original, cisplatin-resistant and doxorubicin-resistant variants). The extracts were administered as 0.1% solution in drinking water (0.6β1.0 mg by total polyphenolics per mouse per day and 4.0β6.3 mg per rat per day). Results: Tumor growth inhibition (TGI) in mice treated with NanoGTE, cisplatin or cisplatin + NanoGTE was 27%, 55% and 78%, respectively, in Sarcoma 180%, 21%, 45% and 59%, respectively, in Ehrlich carcinoma; and 8%, 13% and 38%, respectively in B16 melanoma. Composites of NanoGTE, red wine, and lemon peel (NanoGTRWL) enhanced the antitumor effects of cyclophosphamide in mice with Ca755 mammary carcinoma. The treatment with combination of NanoGTE and inhibitors of polyamines (PA) synthesis (DFMO + MGBG) resulted in significant TGI of P388 leukemia (up to 71%) and L1210 leukemia. In rats transplanted with Guerin carcinoma (parental strain), treatment with GTRW or GTE alone resulted in 25β28% TGI vs. 55β68% TGI in cisplatin-treated animals. The inhibition observed in the case of combination of GTE or GTRW with cisplatin was additive giving 81β88% TGI. Similar effects were observed when combinations of the cytostatics with GTE (or ΒNanoGTE) were tested against cisplatin- or doxorubicin-resistant Guerin carcinoma. Moreover, the plant extracts lowered side toxicity of the drugs. Treatment with GTE, NanoGTE, and NanoGTRW decreased the levels of malondialdehyde in heart, kidney and liver tissue of experimental animals, as well as the levels of urea and creatinine in blood serum, increased erythrocyte and platelet counts, hemoglobin content, and decreased leucocyte counts. Conclusion: The obtained data indicate the prospects for further deveΒlopment of GTE and corresponding nanocomposites as auxiliary agents in cancer chemotherapy. Key Words: polyphenolic plant extracts, antitumor effect, cancer therapy
P123 Additive antitumor effect of plant polyphenols and a synthetic inhibitors of polyamines biosynthesis
Antioxidant And Anticarcinogenic Composite From Green Tea And Red Wine Lee As Food Additive
GREEN TEA, RED WINE AND LEMON EXTRACTS REDUCE EXPERIMENTAL TUMOR GROWTH AND CANCER DRUG TOXICITY
Aim: To evaluate antitumor effect of plant polyphenol extracts from green tea, red wine lees and/or lemon peel alone and in combination with antitumor drugs on the growth of different transplanted tumors in experimental animals. Materials and Methods: Green tea extract (GTE) was prepared from green tea infusion. GTE-based composites of red wine (GTRW), lemon peel (GTRWL) and/or NanoGTE as well as corresponding nanocomposites were prepared. The total polyphenolics of the different GTE-based extracts ranged from 18.0% to 21.3%. The effects of GTE-based extracts were studied in sarcoma 180, Ehrlich carcinoma, B16 melanoma, Ca755 mammary carcinoma, P388 leukemia, L1210 leukemia, and Guerin carcinoma (original, cisplatin-resistant and doxorubicin-resistant variants). The extracts were administered as 0.1% solution in drinking water (0.6β1.0 mg by total polyphenolics per mouse per day and 4.0β6.3 mg per rat per day). Results: Tumor growth inhibition (TGI) in mice treated with NanoGTE, cisplatin or cisplatin + NanoGTE was 27%, 55% and 78%, respectively, in Sarcoma 180%, 21%, 45% and 59%, respectively, in Ehrlich carcinoma; and 8%, 13% and 38%, respectively in B16 melanoma. Composites of NanoGTE, red wine, and lemon peel (NanoGTRWL) enhanced the antitumor effects of cyclophosphamide in mice with Ca755 mammary carcinoma. The treatment with combination of NanoGTE and inhibitors of polyamines (PA) synthesis (DFMO + MGBG) resulted in significant TGI of P388 leukemia (up to 71%) and L1210 leukemia. In rats transplanted with Guerin carcinoma (parental strain), treatment with GTRW or GTE alone resulted in 25β28% TGI vs. 55β68% TGI in cisplatin-treated animals. The inhibition observed in the case of combination of GTE or GTRW with cisplatin was additive giving 81β88% TGI. Similar effects were observed when combinations of the cytostatics with GTE (or ΒNanoGTE) were tested against cisplatin- or doxorubicin-resistant Guerin carcinoma. Moreover, the plant extracts lowered side toxicity of the drugs. Treatment with GTE, NanoGTE, and NanoGTRW decreased the levels of malondialdehyde in heart, kidney and liver tissue of experimental animals, as well as the levels of urea and creatinine in blood serum, increased erythrocyte and platelet counts, hemoglobin content, and decreased leucocyte counts. Conclusion: The obtained data indicate the prospects for further deveΒlopment of GTE and corresponding nanocomposites as auxiliary agents in cancer chemotherapy. Key Words: polyphenolic plant extracts, antitumor effect, cancer therapy