Phylogenetic relationships of GLYI and GLYII from various plant species.

<p>A phylogenetic tree based on the multiple alignments of the GLYI and GLYII amino acid sequences was constructed using MEGA 5.05 software with the Neighbor-Joining method. Bootstrap support from 1,000 reiterations is indicated above the branches. “Br”, “At” and “Os” refer to the GLYI and GLYII proteins in <i>B</i>. <i>rapa</i>, <i>A</i>. <i>thaliana</i> and <i>O sativa</i> (only the first splice variants were considered in the case of multiple members), respectively.</p

Identification of homologous <i>nsLtp</i> genes between <i>A. thaliana</i> and three subgenomes in <i>B. rapa</i>.

a<p><b>CCB, conserved collinear block; ^bLF, the least fractionated blocks of B. </b><b><i>rapa</i></b><b>; ^cMF1, the medium fractionated blocks of B. </b><b><i>rapa</i></b><b>; ^dMF2, the most fractionated blocks of B. </b><b><i>rapa</i></b><b>.</b></p

Summary of the known functions of the <i>GLYI / GLYII</i> genes using a transgenic approach.

<p>Summary of the known functions of the <i>GLYI / GLYII</i> genes using a transgenic approach.</p

Phylogenetic tree of BrnsLtps.

<p>The amino acids of the ECMs were used for Neighbor-Joining phylogenetic tree construction using the MEGA 5.05 software. The ten types of nsLtps and another five AtnsLtps are indicated with circles or triangles of different colors. And the accession number of each gene is showed in the parentheses nearby the corresponding gene name.</p

List of putative <i>BrGLYI</i> and <i>BrGLYII</i> genes along with gene and protein detailed information.

<p>List of putative <i>BrGLYI</i> and <i>BrGLYII</i> genes along with gene and protein detailed information.</p

The workflow of BrnsLtp identification and data mining.

<p>1, BRAD, <i>Brassica</i> Database, <a href="http://brassicadb.org/brad/" target="_blank">http://brassicadb.org/brad/</a>; 2, <a href="http://pfam.sanger.uk/" target="_blank">http://pfam.sanger.uk/</a>; 3, NSS (N-terminal signal sequence) prediction, <a href="http://www.cbs.dtu.dk/services/SignalP" target="_blank">http://www.cbs.dtu.dk/services/SignalP</a>; 4, GPI (glycosylphosphatidylinositol) anchor signal prediction, <a href="http://mendel.imp.ac.at/gpi/plant_server.html" target="_blank">http://mendel.imp.ac.at/gpi/plant_server.html</a> and <a href="http://psort.hgc.jp/form.html" target="_blank">http://psort.hgc.jp/form.html</a>; 5, LTP domain prediction, <a href="http://www.ncbi.nlm.nih.gov/Structure/bwrpsb/bwrpsb.cgi" target="_blank">http://www.ncbi.nlm.nih.gov/Structure/bwrpsb/bwrpsb.cgi</a>.</p

Quantitative RT-PCR analysis for selected <i>BrnsLtp</i> genes in tissues and organs of <i>B. rapa</i> with specific expression patterns.

<p>Quantitative RT-PCR analysis for selected <i>BrnsLtp</i> genes in tissues and organs of <i>B. rapa</i> with specific expression patterns.</p

RT-qPCR assay of the expression of the representative <i>BrGLYI</i> under Cd and Pb treatments.

<p>The y-axis indicates the relative gene expression; the x-axis indicates the different treatments under the control (CK), Cd and Pb conditions.</p

Expression analysis of <i>BrnsLtp</i> genes by UniGene (Transcripts Per Million, TPM).

a<p><b>UN, unigene number; ^bB, bud; ^cF, flower; ^dL, leaf; ^eR, root; ^fS, seed; ^gSi, silique; ^hWP, whole plant; ⁱUT, unspecified tissue; “√” and “–” represent “exist” and “not exist”, respectively. Underlined indicated specific expression.</b></p

Identification of homologous <i>GLYI</i> and <i>GLYII</i> genes between <i>A</i>. <i>thaliana</i> and subgenomes in <i>B</i>. <i>rapa</i>.

<p>Identification of homologous <i>GLYI</i> and <i>GLYII</i> genes between <i>A</i>. <i>thaliana</i> and subgenomes in <i>B</i>. <i>rapa</i>.</p