Expression of Flot-2 and EGFR proteins in lung SCC and lung ADC compared to the control of non-cancerous lung tissues.

<p>Results showed that there were significant differences between the groups which were statistically evaluated by chi-square test.</p

Expression of p-Mnk1 and p-eIF4E protein in NPC and non-cancerous nasopharyngeal epithelial tissues was detected by immunohistochemistry.

<p>The expression of p-Mnk1 and p-eIF4E protein was detected by immunohistochemistry using specific antibodies as described in the section of materials and methods. Strong positive expression of p-Mnk1 was found in nuclei of NPC (Fig. 1A, 20×, IHC, AEC staining). Low and weak nuclear positive expression of p-Mnk1 was showed in the non-cancerous nasopharyngeal epithelial cells (Fig. 1B, 20×, IHC, AEC staining). Strong positive expression of p-eIF4E in the cytoplasm of NPC (Fig. 1C, 20×, IHC, AEC staining). Weak positive expression of p-eIF4E in the cytoplasm of the non-cancerous nasopharyngeal epithelial cells (Fig. 1D, 20×, IHC, AEC staining).</p

Kaplan-Meier overall survival curves of NPC patients with expression of p-Mnk1 and p-eIF4E protein and different clinicopathological characteristics.

<p>Kaplan-Meier analysis to plot the survival curve of all 272 NPC patients with expression of p-Mnk1 and p-eIF4E and different clinicopathological characteristics and statistical significance was assessed using the log-rank test. Fig. 3A. Kaplan-Meier curves showed worse overall survival for p-Mnk1–positive patients compared with p-Mnk1–negative patients (P<0.001, two sided). Fig. 3B. Kaplan-Meier curves showed worse survival for p-eIF4E–positive patients compared with p-eIF4E–negative patients (P = 0.004, two sided). Fig. 3C. NPC patients with clinical stage III and IV were significantly related to poor prognosis compared to those patients with clinical stage I and II (P = 0.003, two sided). Fig. 3D. NPC patients with lymph node metastasis were significantly related to poor prognosis compared to those patients without lymph node metastasis (P<0.001, two sided). Fig. 3E NPC patients with combination chemotherapy and radiotherapy were significantly related to good prognosis compared to patients with chemotherapy and radiotherapy alone (P = 0.006, two sided). Fig. 3F. Histological types of NPC patients were no significantly related to their prognosis (P>0.05, two sided).</p

The pairwise association between expression of p-Mnk1 and p-eIF4E protein in the 272 cases of NPC.

<p>The pairwise association between expression of p-Mnk1 and p-eIF4E protein in the 272 cases of NPC.</p

Expression of EGFR protein in lung SCC cells, lung ADC cells and the control of non-cancerous lung tissue were detected by IHC using specific antibody as described in the section of materials and methods.

<p>Strong positive staining of EGFR protein was found in cell membranes and cytoplasm of lung SCC and lung ADC cells (Fig 2A and 2B, 20×, IHC, DAB staining). Negative staining of EGFR was showed in non-cancerous lung tissue (Fig 2C, 20×, IHC, DAB staining). Negative control showed no EGFR staining in the lung SCC cells (Fig 2D, 20×, IHC, DAB staining).</p

Association between expression of p-Mnk1 and p-eIF4E protein and clinicopathological features of NPC (n = 272).

<p>Abbreviations: NS, non significant; DNKC: Differentiated non-keratinized carcinoma, UDC: Undifferentiated carcinoma; LN, lymph node; LN, LNM, lymph node metastasis.</p

Expression of Flot-2 protein in lung SCC cells, lung ADC cells and control of non-cancerous lung tissues were detected by IHC using specific antibody as described in the section of materials and methods.

<p>Strong positive staining of Flot-2 protein was found on cell membranes of lung SCC and lung ADC cells (Fig 1A and 1B, 20×, IHC, DAB staining). Negative staining of Flot-2 was showed in non-cancerous lung tissue (Fig 1C, 20×, IHC, DAB staining). Negative control showed no Flot-2 staining in lung ADC cells (Fig 1D, 20×, IHC, DAB staining).</p

352 cases of non small cell lung cancer (NSCLC) patient’s characteristics.

<p>352 cases of non small cell lung cancer (NSCLC) patient’s characteristics.</p

Summary of multivariate analysis of Cox proportional hazards model for overall survival in 272 cases of NPC patients.

<p>Abbreviations: DNKC: Differentiated non-keratinized carcinoma, UDC: Undifferentiated carcinoma; LN, lymph node; LNM, lymph node metastasis. Chemotherapy: CT, Radiotherapy: RT.</p

Kaplan-Meier curve for overall survival of NPC patients with expression of Flot-2 and different clinicopathological characteristics.

<p>Kaplan-Meier analysis to plot the overall survival curves of all 134 NPC patients with expression of Flot-2 and different clinicopathological characteristics and statistical significance was assessed using the long-rank test. Fig 3A Expression of Flot-2 protein in the NPC patients was no significantly related to their prognosis (P>0.05, two sided). Fig 3B NPC patients with lymph node metastasis were significantly related to poor prognosis compared to those patients without lymph node metastasis (P = 0.009, two sided). Fig 3C NPC patients with clinical stage III-IV were significantly related to poor prognosis compared to those patients with clinical stage I-II (P = 0.005, two sided).</p