Density effects during sex differentiation in zebrafish (Danio rerio)

Trabajo final presentado por Guillermo Gambús Gubern para obtener el grado, realizado bajo la dirección del Dr. Francesc Piferrer del Institut de Ciències del Mar (ICM-CSIC).-- 24 pagesSexual determination in some fish is still unknown and it has been studied that abiotic factors such as temperature, density, pH and hypoxia have an influence in this process. Based on previous experiments, we analysed density factor in domesticated zebrafish (AB strain), which has a masculinizing effect in a population, subjected to high density treatments. Six different were set up for the experiment, combining low (16 fish/L) and high (66 fish/L) densities in order to determine which period(s) were more sensitive to this factor during zebrafish sexual development having a masculinizing effect, because there were not studies looking for the critical periods. Experiment had a duration from one until 90 days post fertilization, dpf. The results obtained showed that high density treatments had significant differences with low density treatments, having a masculinizing effect. Treatments that spanned (7–18 dpf) and (7–45 dpf) obtained the same percentage of males, making the period (7–18 dpf) the most sensitive during sexual differentiation and having a masculinizing effect, affecting negatively in the growth (body weight and standard length) of this fish speciesPeer Reviewe

Cephalopod biology and care, a COST FA1301 (CephsInAction) training school: anaesthesia and scientific procedures

Lopes, Vanesa... et al.-- 8 pages, 1 tableCephalopods are the sole invertebrates included in the list of regulated species following the Directive 2010/63/EU. According to the Directive, achieving competence through adequate training is a requisite for people having a role in the different functions (article 23) as such carrying out procedures on animals, designing procedures and projects, taking care of animals, killing animals. Cephalopod Biology and Care Training Program is specifically designed to comply with the requirements of the “working document on the development of a common education and training framework to fulfil the requirements under the Directive 2010/63/EU”. The training event occurred at the ICM-CSIC in Barcelona (Spain) where people coming from Europe, America and Asia were instructed on how to cope with regulations for the use of cephalopod molluscs for scientific purposes. The training encompasses discussion on the guidelines for the use and care of animals and their welfare with particular reference to procedures that may be of interest for neuroscience. Intensive discussion has been carried out during the training sessions with focus on behavioural studies and paradigms, welfare assessment, levels of severity of scientific procedures, animal care, handling, transport, individual identification and marking, substance administration, anaesthesia, analgesia and humane killingThe COST Action FA1301 CephsInAction, and the Association for Cephalopod Research supported the Training School “Cephs Biology and Care (CBC-ASP2016)” and the participation of 10 Trainers and 14 Trainees. Sponsorships from Leica and Tecniplast has also been grantedPeer Reviewe