Inhibition of cytokinesis by wiskostatin does not rely on N-WASP/Arp2/3 complex pathway-3

or Arp3 or N-WASP + Arp3 or ECT2. 72 hours after transfection cells were harvested and lysed. N-WASP, Arp3, ECT2, p34 and β-tubulin protein levels were determined by immunoblot analysis using specific antibodies. B. The DNA content of the same HeLa cells was determined by flow cytometry analysis. Arrows indicate abnormal DNA content in ECT2 knockdown cells. A similar scale was used for each histogram. These results are representative of at least three independent experiments. C. Representative DIC images from time-lapse movies of HeLa cells transfected with water (Ø), siRNA directed against N-WASP (N-WASP), against Arp3 (Arp3), against Arp3 and N-WASP (Arp3 + N-WASP) or against ECT2. Images started to be acquired 24 hours post-transfection for 40 hours. Coloured stars indicate the nuclei of dividing cells and of their daughter cells. Time indicated as hours: minutes.<p><b>Copyright information:</b></p><p>Taken from "Inhibition of cytokinesis by wiskostatin does not rely on N-WASP/Arp2/3 complex pathway"</p><p>http://www.biomedcentral.com/1471-2121/9/42</p><p>BMC Cell Biology 2008;9():42-42.</p><p>Published online 30 Jul 2008</p><p>PMCID:PMC2527559.</p><p></p

Inhibition of cytokinesis by wiskostatin does not rely on N-WASP/Arp2/3 complex pathway-0

Etails). Two hours after release from RO3306 block cells were permeabilised and fixed. Cells were treated for indirect Alexa 555 localisation of Arp3 (a, e, and i) and Alexa 350 localisation of β-tubulin (c, g and k) with specific antibodies. F-actin was visualised with FITC-coupled phalloidin (b, f, and j). Merged images are presented (d, h and l). Images are representative of the different stages of mitosis: anaphase (a-d), late telophase (e-h) and cytokinesis (i-l). Bars, 10 μm.<p><b>Copyright information:</b></p><p>Taken from "Inhibition of cytokinesis by wiskostatin does not rely on N-WASP/Arp2/3 complex pathway"</p><p>http://www.biomedcentral.com/1471-2121/9/42</p><p>BMC Cell Biology 2008;9():42-42.</p><p>Published online 30 Jul 2008</p><p>PMCID:PMC2527559.</p><p></p

Inhibition of cytokinesis by wiskostatin does not rely on N-WASP/Arp2/3 complex pathway-2

P-tagged β-tubulin were synchronised in prometaphase with nocodazole and released after a shake-off in presence of vehicle (Cont) or 10 μM wiskostatin (Wisko). 120 minutes after release cells were fixed and F-actin (a and e) and DNA (c and g) stained respectively with Alexa 555-coupled phalloidin and DAPI. β-tubulin (b and f) was directly visualised. Merged images (d and h) are presented. B. Wiskostatin alters Arp3 localisation. HeLa cells were synchronised in G2/M by thymidine and RO3306 double block. 120 minutes after release cells were fixed and stained as described in Figure 1. Images a and e represent staining of Arp3, b and f staining of F-actin and c and g staining of β-tubulin. Merged images (d and h) are presented. Bars, 10 μm.<p><b>Copyright information:</b></p><p>Taken from "Inhibition of cytokinesis by wiskostatin does not rely on N-WASP/Arp2/3 complex pathway"</p><p>http://www.biomedcentral.com/1471-2121/9/42</p><p>BMC Cell Biology 2008;9():42-42.</p><p>Published online 30 Jul 2008</p><p>PMCID:PMC2527559.</p><p></p