Algunas consideraciones sobre las opciones de Centroamérica en sus relaciones comerciales con los Estados Unidos

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Additional file 4: Figure S2. of The lysin motif-containing proteins, Lyp1, Lyk7 and LysMe3, play important roles in chitin perception and defense against Verticillium dahliae in cotton

Silencing of the endogenous Cloroplastos alterados gene (GbCLA1) in cotton through tobacco rattle virus (TRV)-mediated virus-induced gene silencing (VIGS). Eight-day-old cotton seedlings (Hai7124) with two fully expanded cotyledons were infiltrated with TRV: GbCLA1, and the leaf bleaching phenotype was observed 2 weeks later. (TIFF 860 kb

sj-docx-2-inq-10.1177_00469580221134431 – Supplemental material for Evaluation of Online Andrology Medical Services in Central Regions of China During the COVID-19 Pandemic: A Cross-Sectional Data Analysis Study

Supplemental material, sj-docx-2-inq-10.1177_00469580221134431 for Evaluation of Online Andrology Medical Services in Central Regions of China During the COVID-19 Pandemic: A Cross-Sectional Data Analysis Study by Zhi Liu, Chunyu Zhang, Cong Luo, Junlin Ou, Ming Song, Lingxiao Yang, Xin Wang, Jinbo Chen, Guilin Wang and Tao Guo in INQUIRY: The Journal of Health Care Organization, Provision, and Financing</p

sj-docx-1-inq-10.1177_00469580221134431 – Supplemental material for Evaluation of Online Andrology Medical Services in Central Regions of China During the COVID-19 Pandemic: A Cross-Sectional Data Analysis Study

Supplemental material, sj-docx-1-inq-10.1177_00469580221134431 for Evaluation of Online Andrology Medical Services in Central Regions of China During the COVID-19 Pandemic: A Cross-Sectional Data Analysis Study by Zhi Liu, Chunyu Zhang, Cong Luo, Junlin Ou, Ming Song, Lingxiao Yang, Xin Wang, Jinbo Chen, Guilin Wang and Tao Guo in INQUIRY: The Journal of Health Care Organization, Provision, and Financing</p

Variability at DNA methyltransferase loci.

<p>101 N. meningitidis isolates clustered according to SNP distance, yielding in two sequence type (ST) groups. Each column represents an isolate and rows specify the ORF status of 13 DNA methyltransferases (Rebase geneIDs of Z2491 reference strain). Bars in grey at the bottom represent the number of repeat units determining ON/OFF status of the phase-variable modA12 (M.NmeAORF1589P)</p

Methylated nucleotides display higher mutation rates than non-methylated positions.

<p>Positional co-occurrences of (in total 6031) SNPs at positions within (methylation) target motifs. Methylated positions highlighted in red within five target motifs (bold), as detected in the present study, with, each compared to two similar control sequences. Black bars in histogram represent nucleotides in methylated motifs, gray shades represent sequences not known as DNA-methylation targets. For each motif, counts of overlapping SNPs (for 5m-cytosine motifs: C/G in reference →N; or for 6m-adenosine: A/T→N) at each position are normalized by the genome-wide motif occurrences (numbers for methylated motifs in inset box). The dashed lines indicate the corresponding number of SNPs expected from random occurrence (G/C or A/T) across the genome and over-representation was tested with the χ2 statistics (*p value < 10–5).</p

Two N. meningitidis serogroup A strains Z2491 and NM1264 display divergent DNA modifications.

<p>DNA modification scores are plotted against the coverage in SMRT sequencing of Tet1 converted samples. Each dot represents a position on either strand with a modification score larger than 20, the color specifying the nucleotide base, on which the modification was detected. Modified adenosines (red dots) are predominantly detected in strain NM1264. The horizontal line indicates the threshold score 50 applied for subsequent motif finding.</p

Methylation-sensitive restriction assays to validate DNA methylation derived from SMRT sequencing (A) DNA samples separated via gel electrophoresis, lane labels indicating (M) 1 kb Plus DNA Ladder (Invitrogen), (gDNA) whole genomic DNA preparations, and (NlaIV) restriction digest products targeting sites 'GGNNCC' in a methylation-sensitive manner. Samples from 2 different strains (Z2491 and NM1264, genome size: 2.18 Mb) display differential resistance consistent with DNA methylation profiles.(B) single position resolution of modification signal averaged over ~1800 'GGNNCC' sites in the respective genome assemblies. The fractions of sites exhibiting a modification score above 50 are displayed for each position and strand. (C) For comparison, adenosine methylation featuring enhanced sensitivity and positional resolution averaged over ~4000 'ACACC' sites.

<p>Methylation-sensitive restriction assays to validate DNA methylation derived from SMRT sequencing (A) DNA samples separated via gel electrophoresis, lane labels indicating (M) 1 kb Plus DNA Ladder (Invitrogen), (gDNA) whole genomic DNA preparations, and (NlaIV) restriction digest products targeting sites 'GGNNCC' in a methylation-sensitive manner. Samples from 2 different strains (Z2491 and NM1264, genome size: 2.18 Mb) display differential resistance consistent with DNA methylation profiles.(B) single position resolution of modification signal averaged over ~1800 'GGNNCC' sites in the respective genome assemblies. The fractions of sites exhibiting a modification score above 50 are displayed for each position and strand. (C) For comparison, adenosine methylation featuring enhanced sensitivity and positional resolution averaged over ~4000 'ACACC' sites.</p

Phase variability at modA12 locus: Alignment of representative read sequences derived from genome sequencing (strains NM1264 or Z2491).

<p>The number of repeat units is indicated in the second column, with blue cell background indicating a resulting ON status of the ORF, and yellow for an OFF status. The third column indicates the total number of reads matching the corresponding repeat configuration.</p

Depletion of cytosine methylated motifs in the immediate upstream region of ORFs.

<p>Occurrence counts of five (most frequent) methylation target motifs are plotted against their position (in bp) relative to 1997 oriented ORFs (genome annotation N. meningitidis strain Z2491). Motif counts are presented as sum over all ORFs within 50 bp windows, centered at position zero. Red lines in each panel compare to the GC content percentage (y-axis label to the right), averaged over all ORF regions. Dashed horizontal lines represent the averaged motif occurrences corresponding to statistically significant (p value 0.05) depletion of the corresponding motif, derived from a comparison to equally sized sets of random loci. The lower right panel represents occurrence counts of a set of six non-methylated control motifs with similar base composition (identical positions in bold), and similar occurrence frequencies as the non-palindromic target motifs T5mCTGG and AC6mACC. None of the control motifs display significant depletions at ORFs comparable to that of methylated motifs.</p