Evolution of the resistance profile of serial isolates obtained from 24 patients.

<p>For these 24 MDR-TB patients out of 37 with multiple isolates, resistance to additional drugs was observed during treatment. Legend: ▪ = Susceptible, ▪ = Resistant, −Test not performed, I: Isoniazid, R: Rifampicin, E: Ethambutol, Z: Pyrazinamid, Rb: Rifabutin, Ofl: Ofloxacin, Amk: Amikacin, Thio: Thioamide, Cap: Capreomycin NA = Not Applicable as less than 2 isolates were FLP profiled, so no conclusion possible.</p

Number of MDR with first-line drug resistance only, pre-XDR and XDR isolates in the study cohort.

<p>Number of MDR with first-line drug resistance only, pre-XDR and XDR isolates in the study cohort.</p

Characteristics of the 58 MDR-TB patients in strain-clusters and results of epidemiological investigation.

<p>Legend: Y = yes; N = no, U = unknown; − = not applicable.</p

Repartition of the clusters according to the links identified between the patients.

<p>Clusters are represented by unique identification numbers. Next to the cluster number, the number of patients for who an epidemiological link was detected is indicated (i.e. 2/3: two of the three patients included in the cluster present this link). f: familial link; o: same geographic origin; p: geographic proximity. Clusters in orange contain more than 3 patients and cluster in grey more than 6. The purple boxes indicate the number of clusters belonging to each category (or combination of categories) of links. The blue boxes indicate clusters comprising exclusively Belgian-born patients. The green boxes indicate clusters with strains presenting the 776000000000171 spoligotype (S-family).</p

Characteristics of <i>Mycobacterium tuberculosis</i> complex strains isolated from tuberculosis patients living in Brussels and two subgroups; no-clustered and clustered strains, 2010–2013.

<p>Characteristics of <i>Mycobacterium tuberculosis</i> complex strains isolated from tuberculosis patients living in Brussels and two subgroups; no-clustered and clustered strains, 2010–2013.</p

Molecular epidemiology of <i>Mycobacterium tuberculosis</i> complex in Brussels, 2010–2013

<div><p>The tuberculosis (TB) incidence rate in Brussels-Capital Region is 3-fold higher than in Belgium as a whole. Eight years after the realization of initial prospective population-based molecular epidemiology investigations in this Region, a similar study over the period 2010–2013 was conducted. TB strains isolated from 945 patients were submitted to genotyping by standardized 24-locus-MIRU-VNTR typing and spoligotyping. The phylogenetic analysis showed that the LAM (16.7%) and Haarlem (15.7%) branches are the two most prevalent TB lineages circulating in Brussels. Analysis of the MDR subgroup showed an association with Beijing strains (39.9%) and patients native of Eastern Europe (40.7%). Genotyping detected 113 clusters involving 321 patients, giving a recent transmission index of 22.9%. Molecular-guided epidemiological investigations and routine surveillance activities revealed family transmission or social contact for patients distributed over 34 clusters. Most of the patients were foreign-born (75.7%). However, cluster analysis revealed only limited trans-national transmission. Comparison with the previous study shows a stable epidemiological situation except for the mean age difference between Belgian-born and foreign-born patients which has disappeared. This study confirms that molecular epidemiology has become an important determinant for TB control programs. However, sufficient financial means need to be available to perform all required epidemiological investigations.</p></div