Complete genome sequence of burkholderia gladioli myophage mana

Burkholderia gladioli is known to cause respiratory tract infections in cystic fibrosis patients. Here, we describe the annotation of the 38,038-bp genome sequence of Mana, a P2-like phage of B. gladioli. Understanding the genomic characteristics of phages infecting pathogens like B. gladioli can lead to advancements in phage therapy.Fil: Godoy, Brenda. Texas A&M University; Estados UnidosFil: Yao, Guichun. Texas A&M University; Estados UnidosFil: Le, Tram. Texas A&M University; Estados UnidosFil: Vizoso Pinto, María Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Gill, Jason. Texas A&M University; Estados UnidosFil: Gonzalez, Carlos. Texas A&M University; Estados UnidosFil: Liu, Mei. Texas A&M University; Estados Unido

Complete genome sequence of burkholderia gladioli Phage Maja

Burkholderia gladioli is a Gram-negative bacterium associated with cystic fibrosis infections. Here, we describe the genome sequence of B. gladioli phage Maja. Maja is most related to another Burkholderia phage, BcepF1, and may be a temperate phage, despite the absence of repressor or integrase homologs in its genome sequence.Fil: Yu, Zihao. Texas A&M University; Estados UnidosFil: Yao, Guichun. Texas A&M University; Estados UnidosFil: Vizoso Pinto, María Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Sun, Lichang. Jiangsu Academy Of Agricultural Sciences; ChinaFil: Young, Ry. Texas A&M University; Estados UnidosFil: Gonzalez, Carlos. Texas A&M University; Estados UnidosFil: Liu, Mei. Texas A&M University; Estados Unido

Complete genome sequence of burkholderia cenocepacia phage mica

Burkholderia cenocepacia is a multidrug-resistant Gram-negative pathogen known to colonize patients with chronic granulomatous disease and cystic fibrosis. Here, we describe Burkholderia phage Mica, which is predicted to be a lysogenic myophage based on the similarity of its structural proteins to Enterobacteria phage P2 and Burkholderia phage KL3.Fil: Garcia, James. Texas A&M University; Estados UnidosFil: Yao, Guichun. Texas A&M University; Estados UnidosFil: Vizoso Pinto, María Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Clark, James. Texas A&M University; Estados UnidosFil: Le, Tram. Texas A&M University; Estados UnidosFil: Gonzalez, Carlos. Texas A&M University; Estados UnidosFil: Gill, Jason. Texas A&M University; Estados UnidosFil: Liu, Mei. Texas A&M University; Estados Unido

Elevated atmospheric CO2 concentration triggers redistribution of nitrogen to promote tillering in rice

Elevated atmospheric CO2 concentration (eCO2) often reduces nitrogen (N) content in rice plants and stimulates tillering. However, there is a general consensus that reduced N would constrain rice tillering. To resolve this contradiction, we investigated N distribution and transcriptomic changes in different rice plant organs after subjecting them to eCO2 and different N application rates. Our results showed that eCO2 significantly promoted rice tillers (by 0.6, 1.1, 1.7, and 2.1 tillers/plant at 0, 75, 150, and 225 kg N ha−1 N application rates, respectively) and more tillers were produced under higher N application rates, confirming that N availability constrained tillering in the early stages of growth. Although N content declined in the leaves (−11.0 to −20.7 mg g−1) and sheaths (−9.8 to −28.8 mg g−1) of rice plants exposed to eCO2, the N content of newly emerged tillers on plants exposed to eCO2 equaled or exceeded the N content of tillers produced under ambient CO2 conditions. Apparently, the redistribution of N within the plant per se was a critical adaptation strategy to the eCO2 condition. Transcriptomic analysis revealed that eCO2 induced less extensive alteration of gene expression than did N application. Most importantly, the expression levels of multiple N-related transporters and receptors such as nitrate transporter NRT2.3a/b and NRT1.1a/b were differentially regulated in leaf and shoot apical meristem, suggesting that multiple genes were involved in sensing the N signal and transporting N metabolites to adapt to eCO2. The redistribution of N in different organs could be a universal adaptation strategy of terrestrial plants to eCO2

Outbred genome sequencing and CRISPR/Cas9 gene editing in butterflies

Butterflies are exceptionally diverse but their potential as an experimental system has been limited by the difficulty of deciphering heterozygous genomes and a lack of genetic manipulation technology. Here we use a hybrid assembly approach to construct high-quality reference genomes for Papilio xuthus (contig and scaffold N50: 492 kb, 3.4 Mb) and Papilio machaon (contig and scaffold N50: 81 kb, 1.15 Mb), highly heterozygous species that differ in host plant affiliations, and adult and larval colour patterns. Integrating comparative genomics and analyses of gene expression yields multiple insights into butterfly evolution, including potential roles of specific genes in recent diversification. To functionally test gene function, we develop an efficient (up to 92.5%) CRISPR/Cas9 gene editing method that yields obvious phenotypes with three genes, Abdominal-B, ebony and frizzled. Our results provide valuable genomic and technological resources for butterflies and unlock their potential as a genetic model system

International Conference on SEMS 2012

This volume contains the contributed papers of invitees to SEMS 2012 who have also given talks at the conference. The invitees are experts in philosophy of science and technology from Asia (besides China), Australia, Europe, Latin America, North America, as well as from within China. The papers in this volume represent the latest work of each researcher in his or her expertise; and as a result, they give a good representation of the cutting-edge researches in diverse areas in different parts of the world

Automatically Generating Labels Based on Unified Click Model

Ground truth labels are one of the most important parts in many test collections for information retrieval. Each label, depicting the relevance between a query-document pair, is usually judged by a human, and this process is timeconsuming and labor-intensive. Automatically Generating labels from click-through data has attracted increasing attention. In this paper, we propose a Unified Click Model to predict the multi-level labels, which aims at comprehensively considering the advantages of the Position Models and Cascade Models. Experiments show that the proposed click model outperforms the existing click models in predicting the multi-level labels, and could replace the labels judged by humans for test collections

Discovery of a Late Devonian magmatic arc in the southern Lancangjiang zone, western Yunnan: Geochemical and zircon U-Pb geochronological constraints on the evolution of Tethyan ocean basins in SW China

The Tibetan Plateau and western Yunnan are known to have formed by the amalgamation of Gondwana-derived continental blocks and arc terranes as a result of Tethyan subduction followed by continental collisions during the Paleozoic, Mesozoic and Cenozoic. The Devonian and the southern Lancangjiang zone, western Yunnan is a critical period and key region for studying the transformation between the "Proto-Tethyan" and Paleo-Tethyan oceanic systems. New geochemical data and LA-ICPMS U-Pb zircon ages of the Late Devonian volcanogenic sediments from the southern Lancangjiang zone in western Yunnan, SW China, are presented. The studied sedimentary rocks of the Nanguang Formation are volcaniclastic rocks with high volcanic lithic content (55-65%, mostly andesite, dacite, with some rhyolite and tuffs). Whole rock geochemistry, zircon trace elements and detrital modal analyses indicate derivation from a subduction-related magmatic arc. Three tuff samples yield Late Devonian weighted mean 206Pb/238U ages of 378 ± 4 Ma, 366 ± 5 Ma and 382 ± 8 Ma, suggesting a Late Devonian depositional age. 104 zircon U-Pb analyses on 104 zircon grains from two sandstone samples present extremely tight age clusters, mostly ranging from 380 Ma to 360 Ma. This indicates a single Late Devonian igneous source. A short transport distance and a high rate of denudation and deposition within an arc-related basin are considered likely for the tuffs and volcaniclastic rocks in this study. This implies the presence of an as yet unidentified Late Devonian magmatic arc in the southern Lancangjiang zone. The cryptic Late Devonian arc is likely to represent either a continuation of Late Ordovician-Late Silurian "Proto-Tethyan" subduction or the initial stage of the Paleo-Tethyan Lincang Arc and indicates that subduction of the Changning-Menglian ocean beneath the Simao/Indochina Block occurred in the Late Devonian

Progress of Research on the Physiology and Molecular Regulation of Sorghum Growth under Salt Stress by Gibberellin

Plant growth often encounters diverse abiotic stresses. As a global resource-based ecological problem, salinity is widely distributed and one of the major abiotic stresses affecting crop yields worldwide. Sorghum, a cereal crop with medium salt tolerance and great value for the development and utilization of salted soils, is an important source of food, brewing, energy, and forage production. However, in soils with high salt concentrations, sorghum experiences low emergence and suppressed metabolism. It has been demonstrated that the effects of salt stress on germination and seedling growth can be effectively mitigated to a certain extent by the exogenous amendment of hormonal gibberellin (GA). At present, most of the studies on sorghum salt tolerance at home and abroad focus on morphological and physiological levels, including the transcriptome analysis of the exogenous hormone on sorghum salt stress tolerance, the salt tolerance metabolism pathway, and the mining of key salt tolerance regulation genes. The high-throughput sequencing technology is increasingly widely used in the study of crop resistance, which is of great significance to the study of plant resistance gene excavation and mechanism. In this study, we aimed to review the effects of the exogenous hormone GA on leaf morphological traits of sorghum seedlings and further analyze the physiological response of sorghum seedling leaves and the regulation of sorghum growth and development. This review not only focuses on the role of GA but also explores the signal transduction pathways of GA and the performance of their responsive genes under salt stress, thus helping to further clarify the mechanism of regulating growth and production under salt stress. This will serve as a reference for the molecular discovery of key genes related to salt stress and the development of new sorghum varieties