27 research outputs found
Protective Effects of Li-Fei-Xiao-Yan Prescription on Lipopolysaccharide-Induced Acute Lung Injury via Inhibition of Oxidative Stress and the TLR4/NF- Îș
Li-Fei-Xiao-Yan prescription (LFXY) has been clinically used in China to treat inflammatory and infectious diseases including inflammatory lung diseases. The present study was aimed at evaluating the potential therapeutic effects and potential mechanisms of LFXY in a murine model of lipopolysaccharide- (LPS-) induced acute lung injury (ALI). In this study, the mice were orally pretreated with LFXY or dexamethasone (positive drug) before the intratracheal instillation of LPS. Our data indicated that pretreatment with LFXY enhanced the survival rate of ALI mice, reversed pulmonary edema and permeability, improved LPS-induced lung histopathology impairment, suppressed the excessive inflammatory responses via decreasing the expression of proinflammatory cytokines (TNF-α, IL-1ÎČ, and IL-6) and chemokine (MIP-2) and inhibiting inflammatory cells migration, and repressed oxidative stress through the inhibition of MPO and MDA contents and the upregulation of antioxidants (SOD and GSH) activities. Mechanistically, treatment with LFXY significantly prevented LPS-induced TLR4 expression and NF-ÎșB (p65) phosphorylation. Overall, the present study suggests that LFXY protected mice from acute lung injury induced by LPS via inhibition of TLR4/NF-ÎșB p65 activation and upregulation of antioxidative enzymes and it may be a potential preventive and therapeutic agent for ALI in the clinical setting
Type 2 Diabetes Mellitus and Macrovascular Complications
Cet ouvrage Ă©lĂ©gant et raffinĂ©, publiĂ© sous le patronnage de la « FundaciĂłn Fernando VillalĂłn » de MorĂłn de la Frontera (SĂ©ville), reprĂ©sente le couronnement dâune longue sĂ©rie dâĂ©tudes et dâĂ©ditions critiques consacrĂ©es Ă ce poĂšte andalou par Jacques Issorel, hispaniste trĂšs actif et engagĂ©, spĂ©cialiste depuis une bonne vingtaine dâannĂ©es de la personnalitĂ© et de lâĆuvre de Fernando VillalĂłn. On doit Ă son activitĂ© sagace une thĂšse doctorale monumentale, soutenue Ă lâUniversitĂ© Paul ValĂ©ry d..
Micromechanical Modeling of Transport Properties of Cement-Based Composites: Role of Interfacial Transition Zone and Air Voids
International audienceThe transport properties of cement-based composites, including solute diffusivity, electrical conductivity and water permeability, are regarded as durability indicators of cement-based composites. These transport properties are closely related to the microstructure, or rather to the pore structure of materials. Among all the microstructural aspects, the interfacial transition zone (ITZ) between the cement paste matrix and aggregates, and the air voids are believed to play an essential role in the transport properties. However, their impacts on the transport properties are difficult to be quantified. This paper develops a closed-form four-phase micromechanical model accounting for the local properties of ITZ and the saturation states of air voids. The effects of ITZ and air voids on the transport properties of cement-based composites are addressed quantitatively in the model. The KatzâThompson equation is reinterpreted by the model in particular. It is shown that the local properties of ITZ and volume fraction of aggregates act mutually on the overall transport properties, the influence of air voids depends significantly on the water saturation, and a critical saturation degree is found to be 1/3
Early expression of mannose-binding lectin 2 during Aspergillus fumigatus infection in human corneal epithelial cells
<b>AIM:</b> To evaluate the early expression of mannose-binding lectin 2 (MBL2) in human corneal epithelial cells (HCECs) infected by Aspergillus fumigatus (AF).
<b>METHODS:</b> HCECs cultured in vitro with AF antigens and sampled at 0, 0.5, 1, 2, 4, 6 and 8h. The expression of MBL2 mRNA was evaluated by semiquantitative reverse transcription-polymerase chain reaction (RT-PCR). The expression of MBL2 protein in supernatant fluid was shown by enzyme linked immunosorbent assay (ELISA). MBL2 protein in HCECs was detected by immunocytochemistry at 0 and 24h.
<b>RESULTS:</b> MBL2 mRNA and protein are expressed in normal HCECs. The expression of MBL2 mRNA and protein in supernatant fluid begin to increase after being stimulated with AF antigens. The most significantly peak of MBL2 mRNA is in 2h. The protein of MBL2 in supernatant fluid decrease gradually after 0.5h. The protein in HCECs expression increase after stimulation of 24h.
<b>CONCLUSION:</b> MBL2 receptor expressed in normal HCECs in vitro. The stimulation by AF antigens can increase the early expression of it
Chromatin-Remodelling ATPases ISWI and BRM Are Essential for Reproduction in the Destructive Pest Tuta absoluta
The tomato leaf miner (Tuta absoluta) is one of the top 20 plant pests worldwide. We cloned and identified the chromatin-remodelling ATPase genes ISWI and BRM by RACE and bioinformatic analysis, respectively; used RT-qPCR to examine their expression patterns during different life cycle stages; and elucidated their roles in insect reproduction using double-stranded RNA injections. The full-length cDNA of TaISWI was 3428 bp and it encoded a 1025-aa polypeptide. The partial-length cDNA of TaBRM was 3457 bp and it encoded a 1030-aa polypeptide. TaISWI and TaBRM were upregulated at the egg stage. Injection of TaISWI or TaBRM dsRNA at the late pupa stage significantly inhibited adult ovary development and reduced fecundity, hatchability, and longevity in the adult females. To the best of our knowledge, the present study was the first to perform molecular characterisations of two chromatin-remodelling ATPase genes and clarify their roles in T. absoluta fecundity. Chromatin-remodelling ATPases are potential RNAi targets for the control of T. absoluta and other insect pests. The present study was also the first to demonstrate the feasibility of reproductive inhibitory RNAi as a putative approach for the suppression of T. absoluta and other Lepidopteran insect populations
Synthesis and characterization of two new metalâorganic frameworks with interpenetrated structures and luminescent properties
Regioselective Oxidation of Fused-Pentagon Chlorofullerenes
Two
monoxides of typical smaller chlorofullerenes, <sup>#271</sup>C<sub>50</sub>Cl<sub>10</sub>O and <sup>#913</sup>C<sub>56</sub>Cl<sub>10</sub>O, featured with double-fused-pentagons, were synthesized
to demonstrate further regioselective functionalization of non-IPR
(IPR = isolated pentagon rule) chlorofullerenes. Both non-IPR chlorofullerene
oxides exhibit an epoxy structure at the ortho-site of fused pentagons.
In terms of the geometrical analysis and theoretical calculations,
the principles for regioselective epoxy oxidation of non-IPR chlorofullerenes
are revealed to follow both âfused-pentagon ortho-siteâ
and âolefinic bondâ rules, which are valuable for prediction
of oxidation of non-IPR chlorofullerenes
Trisulfide BondâMediated Molecular Phototheranostic Platform for âActivatableâ NIRâII ImagingâGuided Enhanced Gas/ChemoâHypothermal Photothermal Therapy
Abstract Tumor microenvironment (TME)âtriggered phototheranostic platform offers a feasible strategy to improve cancer diagnosis accuracy and minimize treatment side effects. Developing a stable and biocompatible molecular phototheranostic platform for TMEâactivated second nearâinfrared (NIRâII) fluorescence imagingâguided multimodal cascade therapy is a promising strategy for creating desirable anticancer agents. Herein, a new NIRâII fluorescence imagingâguided activatable molecular phototheranostic platform (IRâFEPâRGDâSâSâSâFc) is presented for actively targeted tumor imaging and hydrogen sulfide (H2S) gasâenhanced chemodynamicâhypothermal photothermal combined therapy (CDT/HPTT). It is revealed for the first time that the coupling distance between IRâFE and ferrocene is proportional to the photoinduced electron transfer (PET), and the aqueous environment is favorable for PET generation. The part of CyclicâRGDfK (cRGDfk) peptides can target the tumor and benefit the endocytosis of nanoparticles. The highâconcentration glutathione (GSH) in the TME will separate the fluorescence molecule and ferrocene by the GSHâsensitive trisulfide bond, realizing lightâup NIRâII fluorescence imaging and a cascade of trimodal synergistic CDT/HPTT/gas therapy (GT). In addition, the accumulation of hydroxyl radicals (âąOH) and downâregulation of glutathione peroxidase 4 (GPX4) can produce excessive harmful lipid hydroperoxides, ultimately leading to ferroptosis