N-Methyl-3,5-dinitro­benzamide

The asymmetric unit of the title compound, C8H7N3O5, contains two independent mol­ecules in which the amide plane is oriented at dihedral angles of 29.82 (2) and 31.17 (2)° with respect to the benzene ring. In the crystal, mol­ecules are connected via inter­molecular N—H⋯O hydrogen bonds, forming chains running along the b axis

[1-(4-Chloro­phen­yl)-5-hy­droxy-3-phenyl-1H-pyrazol-4-yl](thio­phen-2-yl)methanone

In the title compound, C20H13ClN2O2S, the chloro­phenyl, phenyl and thienoyl rings are oriented at dihedral angles 17.84 (7), 53.13 (8) and 34.03 (8)°, respectively, to the central pyrazole ring. An intra­molecular O—H⋯O hydrogen bond occurs. In the crystal, pairs of bifurcated O—H⋯O hydrogen bonds link mol­ecules into inversion dimers with R 2 2(12) graph-set motifs

(E)-N′-[(E)-3-Phenyl­allyl­idene]benzo­hydrazide

In the title mol­ecule, C16H14N2O, the dihedral angle between the two phenyl rings is 23.5 (6)°. In the crystal, N—H—O hydrogen bonds link mol­ecules into chains running along the a axis

Evaluation of parameters affecting Agrobacterium-mediated transient expression in citrus

Abstract Agrobacterium-mediated transient expression assays are a convenient alternative to stable expression because they are simple, easy to perform, and achieve gene expression rapidly. This study investigated the factors affecting transient gene expression efficiency in citrus by observing the cryo-sectioning of leaf samples under a laser confocal microscope. These factors included the composition of the infiltration buffer, the Agrobacterium cell density, the leaf development stage, the incubation temperature, and plant genotype. The highest transient expression level of yellow fluorescent protein (YFP) was detected in Mexican lime (Citrus aurantifolia) on the third day after the intermediate-aged leaves were infiltrated with the improved infiltration buffer 1 (15 mmol L−1 2-(N-morpholino) ethanesulfonic acid, 10 mmol L−1 MgCI2, and 200 pmol L−1 acetosyringone), which had an optical density of 0.8 and was incubated at 22°C. Additionally, this transient expression assay was applied to other citrus genotypes. Of note, trifoliate orange (Poncirus trifoliata) and kumquat (Fortunella obovate) had higher expression efficiency than other six genotypes of the Citrus genus. Our study provides research basis for the selection of optimization strategies in transient gene expression and improves the method for available genome investigation in citrus

Multi-gene phylogenetic analyses revealed two novel species and one new record of Trichobotrys (Pleosporales, Dictyosporiaceae) from China

The rotting wood in freshwater is a unique eco-environment favoring various fungi. During our investigation of freshwater fungi on decaying wood, three hyphomycetes were collected from Jiangxi and Guangxi Provinces, China. Based on the morphological observations and phylogenetic analysis of a combined DNA data containing ITS, LSU, SSU and tef1-α sequences, two new Trichobotrys species, T. meilingensis and T. yunjushanensis, as well as a new record of T. effusa, were introduced. Additionally, a comprehensive description of the genus with both morphological and molecular data was first provided

Quantum simulation of topological zero modes on a 41-qubit superconducting processor

Quantum simulation of different exotic topological phases of quantum matter on a noisy intermediate-scale quantum (NISQ) processor is attracting growing interest. Here, we develop a one-dimensional 43-qubit superconducting quantum processor, named as Chuang-tzu, to simulate and characterize emergent topological states. By engineering diagonal Aubry-Andr$\acute{\mathrm{e}}$-Harper (AAH) models, we experimentally demonstrate the Hofstadter butterfly energy spectrum. Using Floquet engineering, we verify the existence of the topological zero modes in the commensurate off-diagonal AAH models, which have never been experimentally realized before. Remarkably, the qubit number over 40 in our quantum processor is large enough to capture the substantial topological features of a quantum system from its complex band structure, including Dirac points, the energy gap's closing, the difference between even and odd number of sites, and the distinction between edge and bulk states. Our results establish a versatile hybrid quantum simulation approach to exploring quantum topological systems in the NISQ era.Comment: Main text: 6 pages, 4 figures; Supplementary: 16 pages, 14 figure

Tunable Coupling Architectures with Capacitively Connecting Pads for Large-Scale Superconducting Multi-Qubit Processors

We have proposed and experimentally verified a tunable inter-qubit coupling scheme for large-scale integration of superconducting qubits. The key feature of the scheme is the insertion of connecting pads between qubit and tunable coupling element. In such a way, the distance between two qubits can be increased considerably to a few millimeters, leaving enough space for arranging control lines, readout resonators and other necessary structures. The increased inter-qubit distance provides more wiring space for flip-chip process and reduces crosstalk between qubits and from control lines to qubits. We use the term Tunable Coupler with Capacitively Connecting Pad (TCCP) to name the tunable coupling part that consists of a transmon coupler and capacitively connecting pads. With the different placement of connecting pads, different TCCP architectures can be realized. We have designed and fabricated a few multi-qubit devices in which TCCP is used for coupling. The measured results show that the performance of the qubits coupled by the TCCP, such as $T_1$ and $T_2$, was similar to that of the traditional transmon qubits without TCCP. Meanwhile, our TCCP also exhibited a wide tunable range of the effective coupling strength and a low residual ZZ interaction between the qubits by properly tuning the parameters on the design. Finally, we successfully implemented an adiabatic CZ gate with TCCP. Furthermore, by introducing TCCP, we also discuss the realization of the flip-chip process and tunable coupling qubits between different chips.Comment: Main text: 7 pages, 6 figure

Antiviral Therapy and Outcomes of Patients with Pneumonia Caused by Influenza A Pandemic (H1N1) Virus

There is limited data on the clinical outcome of patients with pandemic H1N1 (pH1N1) pneumonia who received oseltamivir treatment, especially when the treatment was administered more than 48 hours after symptom onset.During the pandemic in 2009, a cohort of pH1N1 influenza pneumonia was built in China, and their clinical information was collected systematically, and analyzed with Cox models.<200, oseltamivir administration reduced the mortality risk by 92.1%, 88% and 83.5%, respectively. Higher doses of oseltamivir (>3.8 mg/kg/d) did not improve clinical outcome (mortality, higher dose 2.5% vs standard dose 2.8%, p>0.05).<200