The effect of tumour necrosis factor-α (TNF-α) muteins on human neutrophils in vitro

Tumour necrosis factor-α (TNF-α) has been implicated as an important inflammatory mediator. In vitro, TNF-α is reported to activate human polymorphonuclear neutrophils (PMN), inducing responses such as phagocytic activity, degranulation and oxidative metabolism. Biological responses to TNF-α are initiated by its binding to specific cell surface receptors, and various studies have shown that the major TNF receptor species on PMN is the 75 kDa receptor. To verify the suggestion that the receptor binding domain includes the region close to the N-terminus of the TNF-α molecule, four TNF-α derivatives termed muteins were constructed, using a synthetic cDNA fragment substituting the N-terminal 3–7 selected hydrophilic or hydrophobic amino acids in the original TNF-α genomic DNA. Binding of muteins to PMN was assessed using monoclonal antibodies recognizing either the 55 kDa (p55) or the 75 kDa (p75) TNF receptor subtypes. Blocking by muteins of anti-p75 antibody binding to PMN was as expected from their N-terminal amino acid composition and hydrophilic properties. Hydrophilic muteins competed well with anti-TNF receptor antibodies for binding to the p75 receptor. In contrast, hydrophobic muteins were unable to block anti-p75 binding. Similarly, degranulation, chemiluminescence or enhancement of the PMN response to specific stimuli by the muteins correlated with the hydrophilic properties of the muteins. The significance of these observations in relation to the molecular structure of TNF-α is discussed

Trade union strategies in the age of austerity: the Romanian public sector in comparative perspective

This article examines the impact of the economic crisis and its aftermath on collective bargaining, by comparing reactions to austerity policies of trade unions in healthcare and education in Romania. We develop an encompassing theoretical framework that links strategies used by trade unions with power resources, costs and union democracy. In a tight labour market generated by the massive emigration of doctors, unions in healthcare have successfully deployed their resources to advance their interests and obtain significant wage increases and better working conditions. We also show that in the aftermath of the crisis, healthcare trade unions have redefined their strategies and adopted a more militant stance based on a combination of local strikes, strike threats and temporary alliances with various stakeholders. By comparison, we find that unions in the education sector have adopted less effective strategies built around negotiations with governments combined with national-level militancy

Restoration of catalytic functions in Cre recombinase mutants by electrostatic compensation between active site and DNA substrate

Two conserved catalytic arginines, Arg-173 and Arg-292, of the tyrosine site-specific recombinase Cre are essential for the transesterification steps of strand cleavage and joining in native DNA substrates containing scissile phosphate groups. The active site tyrosine (Tyr-324) provides the nucleophile for the cleavage reaction, and forms a covalent 3′-phosphotyrosyl intermediate. The 5′-hydroxyl group formed during cleavage provides the nucleophile for the joining reaction between DNA partners, yielding strand exchange. Previous work showed that substitution of the scissile phosphate (P) by methylphosphonate (MeP) permits strand cleavage by a Cre variant lacking Arg-292. We now demonstrate that MeP activation and cleavage are not blocked by substitution of Arg-173 or even simultaneous substitutions of Arg-173 and Arg-292 by alanine. Furthermore, Cre(R173A) and Cre(R292A) are competent in strand joining, Cre(R173A) being less efficient. No joining activity is detected with Cre(R173A, R292A). Consistent with their ability to cleave and join strands, Cre(R173A) and Cre(R292A) can promote recombination between two MeP-full-site DNA partners. These findings shed light on the overall contribution of active site electrostatics, and tease apart distinctive contributions of the individual arginines, to the chemical steps of recombination. They have general implications in active site mechanisms that promote important phosphoryl transfer reactions in nucleic acids

Tensions in the periphery: Dependence and the trajectory of a low-cost productive model in the Central and Eastern European automotive industry

This article analyses the productive strategy adopted by Renault for its Dacia plant in Romania. It proposes a detailed analysis of the conditions for the success of the Logan project – Renault’s radical approach to the concept of the low-cost automobile. We look into both market- and production-related aspects that have made the Logan work and highlight the tensions sparked by Renault’s drive to capitalize on its favourable market situation as well as the success achieved by Dacia’s workers in defending their interests. In particular, we emphasize the company governance compromises that have shaped industrial relations at Dacia over the past decades and show how in recent years the maintaining of such a compromise has come increasingly into question due to threats by automation and relocation in a context of constantly rising wages and improving working conditions. Finally, we discuss the strategic dilemmas facing both management and labour and their possible resolutions, as well as the relevance of the Dacia case for understanding the future of Central and Eastern Europe as a peripheral region attracting automotive foreign direct investments

Tanzania traditional food recipes

These are healthy traditional food recipes from local foods, featuring green banana (mlali/mnanambo); ripe banana (Ndishi/Mrarao); pigeon pea or small beans (Nganamna /mrondo); maize; finger millet, and other crops from various regions in Tanzania

Energy efficient optimal hop transmission using minimum power least cost algorithm in cooperative routing for wireless sensor network

Cooperative communication has gained a lot of popularity recently. Through a variety of shortest path methods, this article's paradigm may efficiently reduce the amount of power consumed and hop transmission. In this research, we construct the Minimum Power Least Cost Routing (MPLCR) algorithm and evaluate its performance. The design of the proposed algorithm took into account link computation, sequential scanning algorithm approach, and balance (residual) energy. To prevent connection failures and lessen network traffic, the link calculation is used to choose the best route (relay node). In order to reduce network power consumption, a sequential scanning technique was used to find the shortest path with the fewest hops. And also discuss relay nodes and their characteristics in order to improve the transmission stream's quality of service. An ideal path is one that ensures end-to-end transmission while using the least amount of transmitted power. The minimum power least cost routing algorithm uses cooperative communications to help build the smallest power route. When compared to the current algorithms, the proposed approach uses less energy by more than 30%