14 research outputs found
Milk fermented with yogurt cultures and Lactobacillus casei compared with yogurt and gelled milk : influence on intestinal microflora in healthy infants.
International audienc
Development of a Heterologous Model in Germfree Suckling Rats for Studies of Rotavirus Diarrhea
Germfree suckling rats were infected with an SA11 rotavirus strain. Infected pups developed diarrhea associated with histopathological changes. The virus was detected in feces and in the small intestine. Cellular vacuolation was observed in the villi of the jejunum. These results provide a new model for further investigations of group A rotavirus infection
Use of a fermented dairy probiotic drink containing Lactobacillus casei (DN-114 001) to decrease the rate of illness in kids: the DRINK study A patient-oriented, double-blind, cluster-randomized, placebo-controlled, clinical trial
Hazard Identification and Exposure Assessment for Bacterial Risk Assessment of Informally Marketed Milk in Abidjan, Côte d'Ivoire
A Randomized Prospective Double Blind Controlled Trial on Effects of Long-Term Consumption of Fermented Milk Containing Lactobacillus casei in Pre-School Children With Allergic Asthma and/or Rhinitis
To examine whether long-term consumption of fermented
milk containing a specific Lactobacillus casei may improve
the health status of preschool children suffering from allergic asthma
and/or rhinitis a randomized, prospective, double blind, controlled
trial was conducted in 187 children 2–5 y of age. The children
received for 12 mo either fermented milk (100 mL) containing
Lactobacillus casei (108 cfu/mL) or placebo. The time free from and
the number of episodes of asthma/rhinitis after starting intervention
were the outcome measures. The number of fever or diarrhea episodes
and the change in serum immunoglobulin were further assessed.
No statistical difference between intervention and control
group occurred in asthmatic children. In children with rhinitis, the
annual number of rhinitis episodes was lower in the intervention
group, mean difference (95% CI), 1.6 ( 3.15 to 0.05); the mean
duration of an episode of diarrhea was lower in the intervention
group, mean difference 0.81 ( 1.52 to 0.10) days. While longterm
consumption of fermented milk containing Lactobacillus casei
may improve the health status of children with allergic rhinitis no
effect was found in asthmatic children. (Pediatr Res 62: 215–220,
2007
Effects of yogurt and bifidobacteria supplementation on the colonic microbiota in lactose-intolerant subjects
Anti-Infective Activities of Lactobacillus Strains in the Human Intestinal Microbiota: from Probiotics to Gastrointestinal Anti-Infectious Biotherapeutic Agents
Consumption of Lactobacillus casei fermented milk prevents Salmonella reactive arthritis by modulating IL-23/IL-17 expression.
Reactive arthritis is the development of sterile joint inflammation as a sequel to a remote infection, often in the gut. We have previously shown that a low dose of S. enteritidis inoculated to streptomycin-pretreated mice generates a self-limiting enterocolitis suitable for studying reactive arthritis. Here we show that consumption of Lactobacillus casei prior to infection abolishes intestinal and joint inflammation triggered by Salmonella. BALB/c mice were sacrificed after infection; intestinal and joint samples were analyzed for histological changes and expression of cytokines. TNF-α was measured by ELISA and the expression of IL-1β, IL-6, IL-10, IL-17, IL-23 and TGF-β was assessed by qPCR. L. casei consumption prevented Salmonella-induced synovitis, the increment of TNF-α in knees and the increase of IL-17 expression in popliteal and inguinal lymph nodes. At intestinal level consumption of L. casei drastically diminished S. enteritidis invasiveness and shortened splenic persistence of the pathogen. Bacterial loads recovered at days 2 and 5 from Peyer's patches were 10-fold lower in mice fed with L. casei. In accordance, we found that the augment in gut permeability induced during enterocolitis was decreased in those animals. Consumption of L. casei prior to infection failed to increase anti- inflammatory molecules such as IL-10 and TGF-β in the intestine. On the other hand, consumption of L. casei abrogated the expression of TNF-α, IL-17, IL-23, IL-1β and IL-6 in cecum and mesenteric lymph nodes. These cytokines are needed for differentiation of immune cells involved in the development of reactive arthritis such as Th17 and γδ T cells. Trafficking of these inflammatory cells from the gut to the joints has been proposed as a mechanism of generation of reactive arthritis. Our results suggest that L. casei consumption prevents Salmonella-induced synovitis by altering the intestinal milieu necessary for differentiation of cells involved in the generation of joint inflammation