Rescue study of PoxmR361 mutant by the expression of <i>TtPax1/9a</i> and <i>TtPax1/9b</i> genes.

<p>(a) <i>Poxm</i>^<i>R361</i> mutant. (b) <i>Poxm</i>^<i>R361</i> mutant with <i>TtPax1/9a</i> gene expression. (c) <i>Poxm</i>^<i>R361</i> mutant with <i>TtPax1/9b</i> gene expression. Anterior is to the left.</p

Major components from Conserved metazoan signaling pathways identified in <i>T</i>. <i>tridentatus</i>.

<p>The results were generated by BLASTing the unigenes of <i>T</i>. <i>tridentatus</i> to known homologues with the e-value cutoff at 1e-10. Pathway schematics were adapted from KEGG pathway models (<a href="http://www.genome.jp/kegg/" target="_blank">http://www.genome.jp/kegg/</a>). <i>Drosophila melanogaster</i>-specific pathways are colored blue. Genes identified and not identified in <i>T</i>. <i>tridentatus</i> were marked with red font and grey font respectively.</p

Multiple alignment of paired domain of Pax1/9 proteins from representative metazoan.

<p>The polypeptides were aligned using ClustalX 2.1 program and Boxshade online software (<a href="http://www.ch.embnet.org/software/BOX_form.html" target="_blank">http://www.ch.embnet.org/software/BOX_form.html</a>) were employed to highlight conserved amino acids. Conserved DNA binding motifs were underlined. The sequences used in the alignment are as follows: AmPaxA: <i>Acropora millepora</i> PaxA [GenBank: AAC15713.2], BbPax1/9: <i>Branchiostoma belcheri</i> Pax1/9 [GenBank: ABK54274.1], CqPaxA1: <i>Chrysaora quinquecirrha</i> PaxA1 [GenBank: AAB58292.1], DmPoxm: <i>Drosophila melanogaster</i> Poxm [GenBank: ABE69189.1], HlPaxA: <i>Hydra littoralis</i> PaxA [GenBank: AAB58290.1], DrPax1: <i>Danio rerio</i> Pax1 [GenBank: NP_001074061.1], DrPax9: <i>Danio rerio</i> Pax9 [GenBank: NP_571373.1], HsPax1: <i>Homo sapiens</i> Pax1 [GenBank: NP_006183.2], HsPax9: <i>Homo sapiens</i> Pax9 [GenBank: NP_006185.1], MmPax1: <i>Mus musculus</i> Pax1 [GenBank: AAK01146.1], MmPax9: <i>Mus musculus</i> Pax9 [GenBank: NP_035171.1] and NvPaxA: <i>Nematostella vectensis</i> PaxA [GenBank: AAW29066.1].</p

Tissue distribution of <i>TtPax1/9a</i> and <i>TtPax1/9b</i> determined by quantative real-time PCR.

<p>The <i>T</i>. <i>tridentatus GAPDH</i> transcript was used as internal standard. I, intestine; L: liver; Y, yellow connective tissue; H, heart; S: stomach; M, muscle; G, gill.</p

COG functional classification of the <i>T</i>. <i>tridentatus</i> transcriptome.

<p>COG functional classification of the <i>T</i>. <i>tridentatus</i> transcriptome.</p