Ubiquitous Occurrence of Fluorotelomer Alcohols in Eco-Friendly Paper-Made Food-Contact Materials and Their Implication for Human Exposure

The occurrence of fluorotelomer alcohols (FTOHs) was investigated in 94 food-contact materials (FCMs). We detected 6:2 FTOH (<0.60–1110 ng/g), 8:2 FTOH (<0.40–8490 ng/g), and 10:2 FTOH (<0.02–9350 ng/g) in most FCM samples, and four longer-chain C_14–20 FTOHs were, for the first time, identified in FCMs with relatively high concentrations (<0.02–8450 ng/g for 12:2 FTOH, <0.02–1640 ng/g for 14:2 FTOH, <0.02–372 ng/g for 16:2 FTOH, and <0.02–130 ng/g for 18:2 FTOH). There were three typical profiles of FTOHs that were dominated by 6:2 FTOH (95.6 ± 8.1% in 9 FCMs), 8:2 FTOH (50.9 ± 20.8% in 22 FCMs), and 10:2 FTOH (44.5 ± 20.9% in 30 FCMs), indicating the congener-specific usage of FTOHs for different commercial purposes. All nine detectable FCMs produced in the United States were dominated by 6:2 FTOH, which was significantly different from those produced in China. The median concentration of total FTOHs in eco-friendly paper tableware was 2990 ng/g, which was lower than in popcorn bags (18 200 ng/g) but much higher than other FCMs (<0.55–38.7 ng/g). FTOHs could migrate from paper bowls, with migration efficiencies of 0.004–0.24% into water, 0.004–0.24% into 10% ethanol, 0.009–2.79% into 30% ethanol, 0.06–13.0% into 50% ethanol (v/v) simulants, and 0.04–2.28% into oil. Migration efficiencies decreased with increasing carbon chain lengths of FTOHs

Occurrence and Source of Nitrosamines and Secondary Amines in Groundwater and its Adjacent Jialu River Basin, China

The presence of mutagenic and carcinogenic nitrosamines in groundwater is of great concern. In this study, eight nitrosamines including <i>N</i>-nitrosodimethylamine (NDMA), <i>N</i>-nitrosodiethylamine (NDEA), <i>N</i>-nitrosomethylethylamine (NMEA), <i>N</i>-nitrosopyrrolidine (NPYR), <i>N</i>-nitrosomorpholine (NMOR), <i>N</i>-nitrosopiperidine (NPIP), <i>N</i>-nitrosodi-n-propylamine (NDPA), and <i>N</i>-nitrosodi-n-butylamine (NDBA) and corresponding secondary amines were investigated in shallow groundwater, river water, and wastewater samples collected from the Jialu River basin. The total concentrations of nitrosamines and secondary amines in groundwater were ND–101.1 ng/L and 0.36–4.38 μg/L, respectively. NDMA and its secondary amine DMA (44.7%/40.1%) were the predominant compounds in groundwater, followed by NDEA/DEA (21.7%/29.3%) and NDBA/DBA (26.4%/27.4%). Relatively high concentrations of these six compounds were also observed in river water that was influenced by the direct discharge of industrial and domestic wastewater. Using acesulfame as a quantitative population marker, the contribution of domestic sources to the concentrations of nitrosamines and secondary amines was 39–85% in downstream reaches of the Jialu River, and that of industrial sources was estimated to be 65–98% in other sites of the area. Both on-site leakage of domestic and industrial wastewater and leaching from river water would contribute to the occurrence of target pollutants in groundwater. The target pollutants posed a cancer risk of 4.12 × 10^–5 to the local populations due to the direct usage of groundwater as potable water

Biosensor Medaka for Monitoring Intersex Caused by Estrogenic Chemicals

Estrogenic chemicals can induce intersex in fish species leading to disturbance of spermatogenesis and impairment of reproductive success. To overcome the shortcomings of conventional histopathological observation on intersex (low sensitivity, relatively poor accuracy, long experimental periods, as well as laborious and time-consuming), we generated a pMOSP1-EGFP transgenic medaka fish model. In this transgenic fish, the green fluorescence protein (GFP) reporter gene was derived by the regulatory elements of the OSP1 gene, which is a specific and sensitive molecular biomarker for indicating intersex occurrence in male medaka fish exposed to estrogenic chemicals. The transgenic GFP was faithfully expressed in ovaries and in testes with intersex, perfectly mimicking the expression pattern of endogenous OSP1. In intersex testis, the diameters of primary oocytes which could be distinguished by GFP fluorescence observation were as small as 10 μm, lower than that (more than 20 μm) which is observable by histopathology. Using the novel transgenic medaka fish, intersex was observed after 90-day exposure to 0.75 ng/L 17α-ethinyloestradiol (EE₂) (0–90 dph), but only at concentrations of at least 1.38 ng/L EE₂ by histopathology. An effectiveness of a short-term in vivo assay for screening estrogenic chemicals that can monitor intersex appearance at early sex developmental stage (about 30 dph) in male medaka fish was also demonstrated by assessing the intersex induction of EE₂, 17β-estradiol and 4-nonylphenol. This newly developed assay provides an enhanced ability for screening and testing estrogenic chemicals with the potential to induce intersex and studying their biological impacts

Comprehensive Characterization of Organic Light-Emitting Materials in Breast Milk by Target and Suspect Screening

Organic light-emitting materials (OLEMs) are emerging contaminants in the environment and have been detected in various environment samples. However, limited information is available regarding their contamination within the human body. Here, we developed a novel QuEChERS (quick, easy, cheap, effective, rugged, and safe) method coupled with triple quadrupole/high-resolution mass spectrometry to determine OLEMs in breast milk samples, employing both target and suspect screening strategies. Our analysis uncovered the presence of seven out of the 39 targeted OLEMs in breast milk samples, comprising five liquid crystal monomers and two OLEMs commonly used in organic light-emitting diode displays. The cumulative concentrations of the seven OLEMs in each breast milk sample ranged from ND to 1.67 × 103 ng/g lipid weight, with a mean and median concentration of 78.76 and 0.71 ng/g lipid weight, respectively, which were higher compared to that of typical organic pollutants such as polychlorinated biphenyls and polybrominated diphenyl ethers. We calculated the estimated daily intake (EDI) rates of OLEMs for infants aged 0–12 months, and the mean EDI rates during lactation were estimated to range from 30.37 to 54.89 ng/kg bw/day. Employing a suspect screening approach, we additionally identified 66 potential OLEMs, and two of them, cholesteryl hydrogen phthalate and cholesteryl benzoate, were further confirmed using pure reference standards. These two substances belong to cholesteric liquid crystal materials and raise concerns about potential endocrine-disrupting effects, as indicated by in silico predictive models. Overall, our present study established a robust method for the identification of OLEMs in breast milk samples, shedding light on their presence in the human body. These findings indicate human exposure to OLEMs that should be further investigated, including their health risks

Comprehensive Characterization of Organic Light-Emitting Materials in Breast Milk by Target and Suspect Screening

Organic light-emitting materials (OLEMs) are emerging contaminants in the environment and have been detected in various environment samples. However, limited information is available regarding their contamination within the human body. Here, we developed a novel QuEChERS (quick, easy, cheap, effective, rugged, and safe) method coupled with triple quadrupole/high-resolution mass spectrometry to determine OLEMs in breast milk samples, employing both target and suspect screening strategies. Our analysis uncovered the presence of seven out of the 39 targeted OLEMs in breast milk samples, comprising five liquid crystal monomers and two OLEMs commonly used in organic light-emitting diode displays. The cumulative concentrations of the seven OLEMs in each breast milk sample ranged from ND to 1.67 × 103 ng/g lipid weight, with a mean and median concentration of 78.76 and 0.71 ng/g lipid weight, respectively, which were higher compared to that of typical organic pollutants such as polychlorinated biphenyls and polybrominated diphenyl ethers. We calculated the estimated daily intake (EDI) rates of OLEMs for infants aged 0–12 months, and the mean EDI rates during lactation were estimated to range from 30.37 to 54.89 ng/kg bw/day. Employing a suspect screening approach, we additionally identified 66 potential OLEMs, and two of them, cholesteryl hydrogen phthalate and cholesteryl benzoate, were further confirmed using pure reference standards. These two substances belong to cholesteric liquid crystal materials and raise concerns about potential endocrine-disrupting effects, as indicated by in silico predictive models. Overall, our present study established a robust method for the identification of OLEMs in breast milk samples, shedding light on their presence in the human body. These findings indicate human exposure to OLEMs that should be further investigated, including their health risks